FlyBase Allele Report: Dmel\mof[1]

General Information

Symbol

Dmel\mof¹

Species

D. melanogaster

Name

FlyBase ID

FBal0059998

Feature type

allele

Associated gene

Dmel\mof

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Hilfiker et al., 1997)

Progenitor genotype

Cytology

Description

Amino acid replacement: G691E.

(Hilfiker et al., 1997)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

X:5,874,681..5,874,681 [-]

Nucleotide change:

G5874681A

Amino acid change:

G691E | mof-PA

Reported amino acid change:

G691E

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Hilfiker et al., 1997)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased cell death | third instar larval stage

(Bhadra et al., 2012)

lethal | male | recessive

(Hilfiker et al., 1997)

radiation sensitive | larval stage

(Bhadra et al., 2012)

viable | female

(Hilfiker et al., 1997)

Phenotype Manifest In

eye disc | third instar larval stage

(Bhadra et al., 2012)

wing disc | larval stage

(Bhadra et al., 2012)

Detailed Description

Statement

Reference

mof¹ eye imaginal discs of third instar larvae show a substantial level of basal apoptotic cells, above that seen in controls. This high basal level is only slightly increased in mutants following exposure to 25 Gy ionizing radiation, to levels similar to that in controls exposed to the same.

In contrast to controls, mof¹ wing imaginal discs do not show a significant decrease in mitotic indices following exposure to 25 Gy ionizing radiation.

(Bhadra et al., 2012)

Young homozygous mof¹ females generate normal numbers of eggs and fecundity does not drop significantly between 3 and 13 days post-eclosion.

(Yu et al., 2010)

Males derived from a cross of homozygous females to wild-type males show appreciable survival to larval stages (day 5) in the presence of infection with the "male-killing" bacterium S.poulsonii. Infected male embryos derived from a cross of heterozygous females to wild-type males show no survival to day 5.

(Veneti et al., 2005)

Males can develop to the third larval instar or the prepupal stage but fail to metamorphose and to hatch. msl-2^Hsp83.PK females exhibit a very long developmental delay and a significant loss of viability, normal development is restored by removing one functional copy of mof.

(Hilfiker et al., 1997)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

mof¹, nanos^BN has decreased fecundity | female phenotype

(Yu et al., 2010)

Phenotype Manifest In

NOT Enhancer of

Statement

Reference

mof[+]/mof¹ is a non-enhancer of adult thorax | pharate adult stage phenotype of chm¹⁴

(Miotto et al., 2006)

Suppressor of

Statement

Reference

mof¹ is a suppressor of embryonic/larval salivary gland | male | larval stage phenotype of Top2^suo1/Top2^suo3

(Mengoli et al., 2014)

mof¹ is a suppressor of polytene chromosome | male | larval stage phenotype of Top2^suo1/Top2^suo3

(Mengoli et al., 2014)

NOT Suppressor of

Statement

Reference

mof[+]/mof¹ is a non-suppressor of adult thorax | pharate adult stage phenotype of chm¹⁴

(Miotto et al., 2006)

Additional Comments

Genetic Interactions

Statement

Reference

The X polytene chromosome condensation defects observed in larval salivary glands of Top2^suo3/Top2^suo1 males can be rescued by combination with mof¹ in hemizygote state: the X polytene chromosome of mof¹/Y;Top2^suo3/Top2^suo1 double mutants shows normal condensation as well as banding pattern.

(Mengoli et al., 2014)

Doubly homozygous mof¹; nos^BN females are nearly as prolific as nos^BN controls 3-8 days post-eclosion. However, their fecundity drops dramatically 9-13 days post-eclosion.

(Yu et al., 2010)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

mof¹ is rescued by mof^{G4DBD.Tag:FLAG}

(Prestel et al., 2010)

mof¹ is rescued by mof^tub.Tag:TAP

(Mendjan et al., 2006)

mof¹ is rescued by mof^+t10.8

(Hilfiker et al., 1997)

mof¹ is rescued by mof^+t18

(Hilfiker et al., 1997)

mof¹ is rescued by mof^+t6.8

(Hilfiker et al., 1997)

Not rescued by

mof¹ is not rescued by mof^G4DBD.Hsp83

(Schiemann et al., 2010)

mof¹ is not rescued by mof^tHa

(Hilfiker et al., 1997)

Comments

mof¹ rescues the male-specific lethality of mof¹.

(Prestel et al., 2010)

Expression of mof^{Hsp83.T:Ivir\HA1,T:Scer\GAL4-DBD} fails to rescue males from the lethal effects of mof¹.

(Schiemann et al., 2010)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Immunofluorescence determines that msl-1 and msl-2 binding to the X chromosome is reduced, binding of mle is substantially reduced. X-specific isoform of His4 is absent.

(Hilfiker et al., 1997)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

mof-1

(Akhtar and Becker, 2000)

mof¹

(Mengoli et al., 2014, Bhadra et al., 2012, Prestel et al., 2010, Schiemann et al., 2010, Yu et al., 2010, Gelbart et al., 2009, Dahlsveen et al., 2006, Deng and Meller, 2006, Furuhashi et al., 2006, Mendjan et al., 2006)

Name Synonyms

Secondary FlyBase IDs

References (23)

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