FlyBase Allele Report: Dmel\nanos[BN]

General Information

Symbol

Dmel\nanos^BN

Species

D. melanogaster

Name

FlyBase ID

FBal0044220

Feature type

allele

Associated gene

Dmel\nanos

Associated Insertion(s)

P{}nanos^BN

Carried in Construct

Also Known As

nos^bn

Key Links

Allele class

loss of function allele

Mutagen

P-element activity

Nature of the Allele

Allele class

loss of function allele

(Murata and Wharton, 1995)

Mutagen

P-element activity

(Gamberi et al., 2002, Forbes and Lehmann, 1998, Kobayashi et al., 1996)

Progenitor genotype

Associated Insertion(s)

P{}nanos^BN

Cytology

Description

P-element insertion into the 5' region of the nos gene.

(Forbes and Lehmann, 1998)

P-element insertion into the promoter region of nos.

(Kobayashi et al., 1996)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

abdominal segment

(Curtis et al., 1995)

abdominal segment, with nanos^+1+3.Tag:MS2

(Becalska et al., 2011)

embryo | dorsal closure stage

(Gamberi et al., 2002)

embryonic abdominal segment

(Verrotti and Wharton, 2000)

embryonic abdominal segment, with nanos^ΔBX

(Yu et al., 2010)

germline cell

(Deshpande et al., 1999)

primordial germ cell

(Asaoka-Taguchi et al., 1999, Kobayashi et al., 1996)

primordial germ cell | embryonic stage 12 | maternal effect

(Sato et al., 2007)

primordial germ cell | embryonic stage 13 | maternal effect

(Sato et al., 2007)

primordial germ cell | embryonic stage 14 | maternal effect

(Sato et al., 2007)

primordial germ cell | embryonic stage 15 | maternal effect

(Sato et al., 2007)

primordial germ cell | embryonic stage 16 | maternal effect

(Sato et al., 2007)

primordial germ cell | maternal effect

(Hayashi et al., 2004)

Detailed Description

Statement

Reference

nos^BN,nos^{+1+3.T:MS2\MCP.BS}/nos^BN embryos display a reduction in abdominal segmentation, forming an average of 6 of the 8 abdominal segments characteristic of wild-type embryos.

(Becalska et al., 2011)

nos^ΔBX, nos^BN/nos^BN females produce embryos than have a reduction in the average number of abdominal segments.

nos^BN homozygous ovaries are superficially normal.

nos^BN females are considerably less fecund than wild type, but nevertheless produce eggs continuously over an 11-day interval.

(Yu et al., 2010)

Heterozygous embryos do not show segmentation defects.

(Jain and Gavis, 2008)

Approximately 19% of pole cells in embryos derived from nos^BN females are undergoing apoptosis at stage 12-14. Around 33% of pole cells are undergoing apoptosis at stage 14-16.

(Sato et al., 2007)

Beginning at stage 9/10, most pole cells are lost in embryos derived from homozygous females. The pole cells in these embryos sometimes show irregular shapes characteristic of apoptotic cells and 19.7% are TUNEL-positive.

(Hayashi et al., 2004)

Homozygotes show severe head involution defects in only 4% of larvae.

(Gamberi et al., 2002)

The average number of pole cells per embryo is reduced compared to controls in embryos derived from homozygous females.

(Asaoka-Taguchi et al., 1999)

Germ cells clump prematurely and stay in the middle of the embryo.

(Deshpande et al., 1999)

The effects of loss of maternal and zygotic nos product on germ cell migration is studied in females with hb¹⁵ nos^BN mutant germ line clones crossed to nos¹⁸/Df(3R)Dl-FX3 males. Germ cells are formed in the embryos indicating nos function is not required for their formation. nos activity is essential for germ cell migration, from stage 10 onwards. Following exit of the germ cells of the posterior midgut pocket, germ cells fail to migrate over the surface of the gut and instead cluster tightly together on the outer gut surface. Mutant germ cells are of varying size and often have an irregular surface. Zygotic nos expression cannot compensate for the loss of maternal nos. The few germ cells that appear to associate with the somatic embryonic gonad are unable to incorporate into the adult gonad even in the presence of a zygotically active copy of the gene.

(Forbes and Lehmann, 1998)

Pole cells fail to migrate into the gonads. Transplantation of pole cells into hosts that can form normal abdomens demonstrates the pole cells cannot penetrate the gonads. No ovo^D1 females transplanted with nos^BN pole cells produce progeny, also demonstrating the pole cells cannot penetrate the gonads.

(Kobayashi et al., 1996)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

nanos^BN has increased cell death | embryonic stage 14 | maternal effect phenotype, enhanceable by hid^{UASp.Tag:FLAG}/Scer\GAL4^{VP16.nanos.UTR}

(Sato et al., 2007)

nanos^BN has increased cell death | embryonic stage 15 | maternal effect phenotype, enhanceable by hid^{UASp.Tag:FLAG}/Scer\GAL4^{VP16.nanos.UTR}

(Sato et al., 2007)

nanos^BN has increased cell death | embryonic stage 16 | maternal effect phenotype, enhanceable by hid^{UASp.Tag:FLAG}/Scer\GAL4^{VP16.nanos.UTR}

(Sato et al., 2007)

nanos^BN has increased cell death | embryonic stage 13 | maternal effect phenotype, enhanceable by Scer\GAL4^{VP16.nanos.UTR}/Tao^{UASp.Tag:FLAG}

(Sato et al., 2007)

nanos^BN has increased cell death | embryonic stage 14 | maternal effect phenotype, enhanceable by Scer\GAL4^{VP16.nanos.UTR}/Tao^{UASp.Tag:FLAG}

(Sato et al., 2007)

nanos^BN has increased cell death | embryonic stage 15 | maternal effect phenotype, enhanceable by Scer\GAL4^{VP16.nanos.UTR}/Tao^{UASp.Tag:FLAG}

(Sato et al., 2007)

nanos^BN has increased cell death | embryonic stage 16 | maternal effect phenotype, enhanceable by Scer\GAL4^{VP16.nanos.UTR}/Tao^{UASp.Tag:FLAG}

(Sato et al., 2007)

nanos^BN has increased cell death | embryonic stage 13 | maternal effect phenotype, enhanceable by skl^UASp.cSa/Scer\GAL4^{VP16.nanos.UTR}

(Sato et al., 2007)

nanos^BN has increased cell death | embryonic stage 14 | maternal effect phenotype, enhanceable by skl^UASp.cSa/Scer\GAL4^{VP16.nanos.UTR}

(Sato et al., 2007)

nanos^BN has increased cell death | embryonic stage 15 | maternal effect phenotype, enhanceable by skl^UASp.cSa/Scer\GAL4^{VP16.nanos.UTR}

(Sato et al., 2007)

nanos^BN has increased cell death | embryonic stage 16 | maternal effect phenotype, enhanceable by skl^UASp.cSa/Scer\GAL4^{VP16.nanos.UTR}

(Sato et al., 2007)

Suppressed by

Statement

Reference

Scer\GAL4^{VP16.nanos.UTR}, Tao^{UASp.Tag:FLAG}, nanos^BN has increased cell death | maternal effect | embryonic stage 13 phenotype, suppressible by hid⁰⁵⁰¹⁴/Df(3L)H99

(Sato et al., 2007)

Scer\GAL4^{VP16.nanos.UTR}, Tao^{UASp.Tag:FLAG}, nanos^BN has increased cell death | maternal effect | embryonic stage 14 phenotype, suppressible by hid⁰⁵⁰¹⁴/Df(3L)H99

(Sato et al., 2007)

Scer\GAL4^{VP16.nanos.UTR}, Tao^{UASp.Tag:FLAG}, nanos^BN has increased cell death | maternal effect | embryonic stage 15 phenotype, suppressible by hid⁰⁵⁰¹⁴/Df(3L)H99

(Sato et al., 2007)

Scer\GAL4^{VP16.nanos.UTR}, Tao^{UASp.Tag:FLAG}, nanos^BN has increased cell death | maternal effect | embryonic stage 16 phenotype, suppressible by hid⁰⁵⁰¹⁴/Df(3L)H99

(Sato et al., 2007)

Scer\GAL4^{VP16.nanos.UTR}, nanos^BN, skl^UASp.cSa has increased cell death | maternal effect | embryonic stage 13 phenotype, suppressible by hid⁰⁵⁰¹⁴/Df(3L)H99

(Sato et al., 2007)

Scer\GAL4^{VP16.nanos.UTR}, nanos^BN, skl^UASp.cSa has increased cell death | maternal effect | embryonic stage 14 phenotype, suppressible by hid⁰⁵⁰¹⁴/Df(3L)H99

(Sato et al., 2007)

Scer\GAL4^{VP16.nanos.UTR}, nanos^BN, skl^UASp.cSa has increased cell death | maternal effect | embryonic stage 15 phenotype, suppressible by hid⁰⁵⁰¹⁴/Df(3L)H99

(Sato et al., 2007)

Scer\GAL4^{VP16.nanos.UTR}, nanos^BN, skl^UASp.cSa has increased cell death | maternal effect | embryonic stage 16 phenotype, suppressible by hid⁰⁵⁰¹⁴/Df(3L)H99

(Sato et al., 2007)

nanos^BN has increased cell death | maternal effect | embryonic stage 12 phenotype, suppressible by hid⁰⁵⁰¹⁴/Df(3L)H99

(Sato et al., 2007)

nanos^BN has increased cell death | maternal effect | embryonic stage 13 phenotype, suppressible by hid⁰⁵⁰¹⁴/Df(3L)H99

(Sato et al., 2007)

nanos^BN has increased cell death | maternal effect | embryonic stage 14 phenotype, suppressible by hid⁰⁵⁰¹⁴/Df(3L)H99

(Sato et al., 2007)

nanos^BN has increased cell death | maternal effect | embryonic stage 13 phenotype, suppressible by Scer\GAL4^{VP16.nanos.UTR}/Tao^{D168A.UASp.Tag:FLAG}

(Sato et al., 2007)

nanos^BN has increased cell death | maternal effect | embryonic stage 14 phenotype, suppressible by Scer\GAL4^{VP16.nanos.UTR}/Tao^{D168A.UASp.Tag:FLAG}

(Sato et al., 2007)

nanos^BN has increased cell death | maternal effect | embryonic stage 15 phenotype, suppressible by Scer\GAL4^{VP16.nanos.UTR}/Tao^{D168A.UASp.Tag:FLAG}

(Sato et al., 2007)

nanos^BN has increased cell death | maternal effect | embryonic stage 16 phenotype, suppressible by Scer\GAL4^{VP16.nanos.UTR}/Tao^{D168A.UASp.Tag:FLAG}

(Sato et al., 2007)

Enhancer of

Statement

Reference

nanos^BN is an enhancer of partially lethal - majority die | embryonic stage | maternal effect | germline clone phenotype of hb¹⁵

(Deshpande et al., 2005)

Suppressor of

Statement

Reference

nanos^BN/nanos^L7 is a suppressor | partially of abnormal size | oogenesis phenotype of l(3)mbt^GM76/Df(3R)ED10966

(Coux et al., 2018)

nanos^BN/nanos^L7 is a suppressor | partially of increased cell number | oogenesis phenotype of l(3)mbt^GM76/Df(3R)ED10966

(Coux et al., 2018)

NOT Suppressor of

Statement

Reference

nanos^BN/nanos^L7 is a non-suppressor | partially of abnormal size | oogenesis phenotype of l(3)mbt^GM76/Df(3R)ED10966

(Coux et al., 2018)

Other

Statement

Reference

mof¹, nanos^BN has decreased fecundity | female phenotype

(Yu et al., 2010)

nanos^BN, nsl1⁰⁶⁵³⁶ has decreased fecundity | female | progressive phenotype

(Yu et al., 2010)

nanos^BN, nsl1^c04892 has decreased fecundity | female | progressive phenotype

(Yu et al., 2010)

nanos^BN, nsl1^+t9.9, nsl1¹ has decreased fecundity | female | progressive phenotype

(Yu et al., 2010)

nanos^BN, nsl1¹ has female semi-sterile phenotype

(Yu et al., 2010)

nanos^BN, nsl1¹ has decreased fecundity | female | progressive phenotype

(Yu et al., 2010)

Phenotype Manifest In

Enhanced by

Statement

Reference

nanos^+1+3.Tag:MS2, nanos^BN has abdominal segment phenotype, enhanceable by Df(2L)BSC32/+

(Becalska et al., 2011)

nanos^+1+3.Tag:MS2, nanos^BN has abdominal segment phenotype, enhanceable by aub[+]/aub^QC42

(Becalska et al., 2011)

nanos^+1+3.Tag:MS2, nanos^BN has abdominal segment phenotype, enhanceable by aub[+]/aub^HN2

(Becalska et al., 2011)

nanos^ΔBX, nanos^BN has embryonic abdominal segment phenotype, enhanceable by nsl1¹/wah[+]

(Yu et al., 2010)

nanos^ΔBX, nanos^BN has embryonic abdominal segment phenotype, enhanceable by nsl1⁰⁶⁵³⁶/wah[+]

(Yu et al., 2010)

nanos^ΔBX, nanos^BN has embryonic abdominal segment phenotype, enhanceable by wah[+]/nsl1^c04892

(Yu et al., 2010)

nanos^ΔBX, nanos^BN has embryonic abdominal segment phenotype, enhanceable by Df(3R)Exel8162/+

(Yu et al., 2010)

nanos^BN has primordial germ cell | embryonic stage 14 | maternal effect phenotype, enhanceable by hid^{UASp.Tag:FLAG}/Scer\GAL4^{VP16.nanos.UTR}

(Sato et al., 2007)

nanos^BN has primordial germ cell | embryonic stage 15 | maternal effect phenotype, enhanceable by hid^{UASp.Tag:FLAG}/Scer\GAL4^{VP16.nanos.UTR}

(Sato et al., 2007)

nanos^BN has primordial germ cell | embryonic stage 16 | maternal effect phenotype, enhanceable by hid^{UASp.Tag:FLAG}/Scer\GAL4^{VP16.nanos.UTR}

(Sato et al., 2007)

nanos^BN has primordial germ cell | embryonic stage 13 | maternal effect phenotype, enhanceable by Scer\GAL4^{VP16.nanos.UTR}/Tao^{UASp.Tag:FLAG}

(Sato et al., 2007)

nanos^BN has primordial germ cell | embryonic stage 14 | maternal effect phenotype, enhanceable by Scer\GAL4^{VP16.nanos.UTR}/Tao^{UASp.Tag:FLAG}

(Sato et al., 2007)

nanos^BN has primordial germ cell | embryonic stage 15 | maternal effect phenotype, enhanceable by Scer\GAL4^{VP16.nanos.UTR}/Tao^{UASp.Tag:FLAG}

(Sato et al., 2007)

nanos^BN has primordial germ cell | embryonic stage 16 | maternal effect phenotype, enhanceable by Scer\GAL4^{VP16.nanos.UTR}/Tao^{UASp.Tag:FLAG}

(Sato et al., 2007)

nanos^BN has primordial germ cell | embryonic stage 13 | maternal effect phenotype, enhanceable by skl^UASp.cSa/Scer\GAL4^{VP16.nanos.UTR}

(Sato et al., 2007)

nanos^BN has primordial germ cell | embryonic stage 14 | maternal effect phenotype, enhanceable by skl^UASp.cSa/Scer\GAL4^{VP16.nanos.UTR}

(Sato et al., 2007)

nanos^BN has primordial germ cell | embryonic stage 15 | maternal effect phenotype, enhanceable by skl^UASp.cSa/Scer\GAL4^{VP16.nanos.UTR}

(Sato et al., 2007)

nanos^BN has primordial germ cell | embryonic stage 16 | maternal effect phenotype, enhanceable by skl^UASp.cSa/Scer\GAL4^{VP16.nanos.UTR}

(Sato et al., 2007)

nanos^ΔBX, nanos^BN has embryonic abdominal segment | maternal effect phenotype, enhanceable | maternal effect by Hsp83^A133D/Hsp83[+]

(Song et al., 2007)

nanos^ΔBX, nanos^BN has embryonic abdominal segment | maternal effect phenotype, enhanceable | maternal effect by Hsp83^e6D/Hsp83[+]

(Song et al., 2007)

nanos^ΔBX, nanos^BN has embryonic abdominal segment | maternal effect phenotype, enhanceable | maternal effect by Hsp83[+]/Hsp83^e6A

(Song et al., 2007)

NOT Enhanced by

Statement

Reference

nanos^+1+3.Tag:MS2, nanos^BN has abdominal segment phenotype, non-enhanceable by squ[+]/squ^HE47

(Becalska et al., 2011)

nanos^ΔBX, nanos^BN has embryonic abdominal segment | maternal effect phenotype, non-enhanceable by Hsp83^j5C2/Hsp83[+]

(Song et al., 2007)

Suppressed by

Statement

Reference

Scer\GAL4^{VP16.nanos.UTR}, nanos^BN, skl^UASp.cSa has primordial germ cell | maternal effect | embryonic stage 16 phenotype, suppressible by hid⁰⁵⁰¹⁴/Df(3L)H99

(Sato et al., 2007)

nanos^BN has primordial germ cell | maternal effect | embryonic stage 12 phenotype, suppressible by hid⁰⁵⁰¹⁴/Df(3L)H99

(Sato et al., 2007)

Scer\GAL4^{VP16.nanos.UTR}, Tao^{UASp.Tag:FLAG}, nanos^BN has primordial germ cell | maternal effect | embryonic stage 13 phenotype, suppressible by hid⁰⁵⁰¹⁴/Df(3L)H99

(Sato et al., 2007)

nanos^BN has primordial germ cell | maternal effect | embryonic stage 13 phenotype, suppressible by hid⁰⁵⁰¹⁴/Df(3L)H99

(Sato et al., 2007)

nanos^BN has primordial germ cell | maternal effect | embryonic stage 14 phenotype, suppressible by hid⁰⁵⁰¹⁴/Df(3L)H99

(Sato et al., 2007)

nanos^BN has primordial germ cell | maternal effect | embryonic stage 13 phenotype, suppressible by Scer\GAL4^{VP16.nanos.UTR}/Tao^{D168A.UASp.Tag:FLAG}

(Sato et al., 2007)

nanos^BN has primordial germ cell | maternal effect | embryonic stage 14 phenotype, suppressible by Scer\GAL4^{VP16.nanos.UTR}/Tao^{D168A.UASp.Tag:FLAG}

(Sato et al., 2007)

nanos^BN has primordial germ cell | maternal effect | embryonic stage 15 phenotype, suppressible by Scer\GAL4^{VP16.nanos.UTR}/Tao^{D168A.UASp.Tag:FLAG}

(Sato et al., 2007)

nanos^BN has primordial germ cell | maternal effect | embryonic stage 16 phenotype, suppressible by Scer\GAL4^{VP16.nanos.UTR}/Tao^{D168A.UASp.Tag:FLAG}

(Sato et al., 2007)

Scer\GAL4^{VP16.nanos.UTR}, Tao^{UASp.Tag:FLAG}, nanos^BN has primordial germ cell | maternal effect | embryonic stage 14 phenotype, suppressible by hid⁰⁵⁰¹⁴/Df(3L)H99

(Sato et al., 2007)

Scer\GAL4^{VP16.nanos.UTR}, Tao^{UASp.Tag:FLAG}, nanos^BN has primordial germ cell | maternal effect | embryonic stage 15 phenotype, suppressible by hid⁰⁵⁰¹⁴/Df(3L)H99

(Sato et al., 2007)

Scer\GAL4^{VP16.nanos.UTR}, Tao^{UASp.Tag:FLAG}, nanos^BN has primordial germ cell | maternal effect | embryonic stage 16 phenotype, suppressible by hid⁰⁵⁰¹⁴/Df(3L)H99

(Sato et al., 2007)

Scer\GAL4^{VP16.nanos.UTR}, nanos^BN, skl^UASp.cSa has primordial germ cell | maternal effect | embryonic stage 13 phenotype, suppressible by hid⁰⁵⁰¹⁴/Df(3L)H99

(Sato et al., 2007)

Scer\GAL4^{VP16.nanos.UTR}, nanos^BN, skl^UASp.cSa has primordial germ cell | maternal effect | embryonic stage 14 phenotype, suppressible by hid⁰⁵⁰¹⁴/Df(3L)H99

(Sato et al., 2007)

Scer\GAL4^{VP16.nanos.UTR}, nanos^BN, skl^UASp.cSa has primordial germ cell | maternal effect | embryonic stage 15 phenotype, suppressible by hid⁰⁵⁰¹⁴/Df(3L)H99

(Sato et al., 2007)

nanos^BN has phenotype, suppressible by Dvir\nanos^tCa

(Curtis et al., 1995)

NOT suppressed by

Statement

Reference

nanos^ΔBX, nanos^BN has embryonic abdominal segment | maternal effect phenotype, non-suppressible by Hsp83^j5C2/Hsp83[+]

(Song et al., 2007)

nanos^BN has abdominal segment phenotype, non-suppressible by Hmag\nanos1^nos.UTR

(Mochizuki et al., 2000)

Enhancer of

Statement

Reference

nanos^BN/nanos^+1+3.Tag:MS2 is an enhancer of abdominal segment phenotype of rump¹

(Becalska et al., 2011)

nanos^BN/nanos^+1+3.Tag:MS2 is an enhancer of abdominal segment phenotype of aub[+]/aub^QC42, rump¹

(Becalska et al., 2011)

nanos^BN/nos[+] is an enhancer of embryonic abdominal segment phenotype of rump¹

(Jain and Gavis, 2008)

Suppressor of

Statement

Reference

nanos^BN/nanos^L7 is a suppressor | partially of egg chamber phenotype of lky^KO

(Antel et al., 2022)

nanos^BN/nanos^L7 is a suppressor | partially of ovary phenotype of l(3)mbt^GM76/Df(3R)ED10966

(Coux et al., 2018)

nanos^BN/nanos^L7 is a suppressor | partially of ovariole phenotype of l(3)mbt^GM76/Df(3R)ED10966

(Coux et al., 2018)

nanos^BN/nanos^L7 is a suppressor | partially of egg chamber phenotype of l(3)mbt^GM76/Df(3R)ED10966

(Coux et al., 2018)

nanos^BN/nanos^L7 is a suppressor | partially of female germline cell | increased number phenotype of l(3)mbt^GM76/Df(3R)ED10966

(Coux et al., 2018)

nanos^BN/nanos^L7 is a suppressor | partially of nurse cell | increased number phenotype of l(3)mbt^GM76/Df(3R)ED10966

(Coux et al., 2018)

nanos^BN/nanos^L7 is a suppressor | partially of oocyte | increased number phenotype of l(3)mbt^GM76/Df(3R)ED10966

(Coux et al., 2018)

nanos^BN/nanos^L7 is a suppressor | partially of follicle cell phenotype of l(3)mbt^GM76/Df(3R)ED10966

(Coux et al., 2018)

Other

Statement

Reference

nanos^BN, nsl1¹ has ovary phenotype

(Yu et al., 2010)

nanos^BN, nsl1¹ has female germline cyst phenotype

(Yu et al., 2010)

Sxl^fP7B0, nanos^BN has germline cell phenotype

(Deshpande et al., 1999)

Additional Comments

Genetic Interactions

Statement

Reference

nos^BN,nos^{+1+3.T:MS2\MCP.BS}/nos^BN embryos carrying a Df(2L)BSC32/+ mutation display a reduction in abdominal segmentation, forming an average of 2.4 abdominal segments.

nos^BN,nos^{+1+3.T:MS2\MCP.BS}/nos^BN embryos carrying a aub^QC42/+ mutation display a reduction in abdominal segmentation, forming an average of 2-3 abdominal segments. Mutant embryos display the normal number of pole cells.

nos^BN,nos^{+1+3.T:MS2\MCP.BS}/nos^BN embryos carrying a aub^HN/+ mutation display a reduction in abdominal segmentation, forming an average of 3-4 abdominal segments.

nos^BN,nos^{+1+3.T:MS2\MCP.BS}/nos^BN embryos carrying a squ^HE47/+ mutation do not display a reduction in abdominal segmentation compared to nos^BN,nos^{+1+3.T:MS2\MCP.BS}/nos^BN embryos.

rump¹ mutant embryos carrying a nos^BN, nos^{+1+3.T:MS2\MCP.BS}/nos^BN mutation display an increase in the number of embryos that develop less than 8 abdominal segments.

rump¹ mutant embryos carrying an aub^QC42/+ mutation, and the mutation nos^BN, nos^{+1+3.T:MS2\MCP.BS}/nos^BN , display an increase in the number of embryos that develop less than 8 abdominal segments.

(Becalska et al., 2011)

nos^ΔBX, nos^BN/nos^BN females that are also heterozygous for wah¹ give rise to embryos that rarely hatch, concomitant with a reduction in the average number of abdominal segments from 3.7 to 1.9.

nos^ΔBX, nos^BN/nos^BN females that are also heterozygous for wah⁰⁶⁵³⁶, wah^c04892 or Df(3R)Exel8162 give rise to embryos with a reduction in the average number of abdominal segments.

wah¹ nos^BN/nos^BN females appear to be significantly less fertile than nos^BN/nos^BN females.

In contrast to nos^BN homozygous ovaries, ovaries from wah¹ nos^BN/nos^BN females have few normal ovarioles: many contain either no maturing egg chambers or only a single mature stage 13/14 oocyte; and many contain aberrant, small egg chambers that contain only one or two highly polyploid germ line nuclei.

In contrast to nos^BN/nos^BN females, wah¹, nos^BN/nos^BN females produce eggs for only 5-6 days after eclosion.

wah⁰⁶⁵³⁶, nos^BN/nos^BN females exhibit more than tenfold lower fecundity between days 9 and 13 than between days 3 and 8.

wah^c04892, nos^BN/nos^BN females exhibit more than tenfold lower fecundity between days 9 and 13 than between days 3 and 8.

wah¹, nos^BN/nos^L7 females do not exhibit age-dependent loss of fecundity.

A single copy of wah^+t9.9 can rescue the age-dependent female infertility of wah¹, nos^BN/nos^BN females. However, wah¹, nos^BN homozygotes expressing a single copy of wah^+t9.9 exhibit age-dependent infertility, producing no eggs after the 8th day post-eclosion.

In ovarioles from very young wah¹, nos^BN/nos^BN flies, the spectrosomes of germline stem cells and cystoblasts and the fusomes of immature cysts appear normal. But slightly older ovaries are bereft of germline cells: some germaria contain only one or two germline cysts and many have none. In these older ovaries, differentiation or development of the few remaining cysts is abnormal, many of which contain one or two large polyploid cells.

Doubly homozygous mof¹; nos^BN females are nearly as prolific as nos^BN controls 3-8 days post-eclosion. However, their fecundity drops dramatically 9-13 days post-eclosion.

(Yu et al., 2010)

The frequency and severity of abdominal segmentation defects in embryos derived from rump¹ homozygous females is increased by one copy of nos^BN. Abdominal segments 4 and 5 are most frequently affected.

The ability of nos^nos.+2.Hsp83 to rescue abdominal segmentation of nos^BN embryos is severely compromised in a rump¹/Df(3R)by416 background; more than 90% of the embryos develop fewer than 4 abdominal segments.

(Jain and Gavis, 2008)

Removal of zygotic W (W⁰⁵⁰¹⁴/Df(3L)H99) suppresses the pole cell apoptosis seen in embryos derived from nos^BN females. Approximately 7% of pole cells examined at stage 12-14 are undergoing apoptosis.

Expression of W^{Scer\UAS.P\T.T:Zzzz\FLAG} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} enhances the pole cell apoptosis seen in stage 14-16 embryos derived from nos^BN females.

Expression of Tao-1^{D168A.Scer\UAS.P\T.T:Zzzz\FLAG} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} suppresses the pole cell apoptosis seen in stage 13-16 embryos derived from nos^BN females.

Expression of Tao-1^{Scer\UAS.P\T.T:Zzzz\FLAG} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} enhances the pole cell apoptosis seen in stage 13-16 embryos derived from nos^BN females.

Expression of skl^{Scer\UAS.P\T.cSa} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} enhances the pole cell apoptosis seen in stage 13-16 embryos derived from nos^BN females.

Removal of zygotic W (W⁰⁵⁰¹⁴/Df(3L)H99) suppresses the pole cell apoptosis seen when Tao-1^{Scer\UAS.P\T.T:Zzzz\FLAG} is expressed under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} in stage 13-16 embryos derived from nos^BN females.

Removal of zygotic W (W⁰⁵⁰¹⁴/Df(3L)H99) suppresses the pole cell apoptosis seen when skl^{Scer\UAS.P\T.cSa} is expressed under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} in stage 13-16 embryos derived from nos^BN females.

(Sato et al., 2007)

Only about 55% of the progeny of hb¹⁵; nos^BN germ-line clone mothers mated to wild-type fathers hatch into first instar larvae, but most of those that hatch make it to adulthood (42% of all progeny). This embryonic lethality is rescued by nos^+t5.7.

(Deshpande et al., 2005)

Homozygous Df(3L)H99 embryos that are derived from nos^BN homozygous females show a suppression of the apoptosis of the pole cells that is seen in embryos derived from nos^BN homozygous females mated to wild-type males. These rescued "pole cells" (from homozygous Df(3L)H99 embryos that are derived from nos^BN homozygous females) can become integrated into somatic tissues, such as midgut epithelium, tracheal epithelium and gastric caeca, when transplanted into host embryos (this is not seen with either control pole cells or pole cells derived from nos^BN homozygous females mated to wild-type males). The pole cells that are integrated into somatic tissues are morphologically indistinguishable from their neighbouring host cells. In addition, the rescued "pole cells" are incorporated into gonads in approximately 25% of cases, when transplanted into host embryos (this phenotype is not seen when pole cells derived from nos^BN homozygous females mated to wild-type males are transplanted into host embryos). These pole cells are morphologically indistinguishable from the host pole cells, but do not contribute to egg or sperm production.

(Hayashi et al., 2004)

No genetic interaction in terms of abdominal segmentation is seen in the cup^unspecified/+ ; nos^BN combination. No genetic interaction in terms of abdominal segmentation is seen in the cup^unspecified/nos^ΔBX ; nos^BN combination.

(Verrotti and Wharton, 2000)

When embryos are also mutant for Sxl^fP7B0, the germ cells form two clusters instead of one.

(Deshpande et al., 1999)

Xenogenetic Interactions

Statement

Reference

Hmag\nos1^nos.UTR fails to rescue the abdominal defects of embryos derived from nos^BN females.

(Mochizuki et al., 2000)

Two copies of P{Dvnos} partially rescues the mutant phenotype.

(Curtis et al., 1995)

Complementation and Rescue Data

Rescued by

nanos^BN is rescued by nanos^nanos.+1.+2'

(Bergsten et al., 2001)

nanos^BN is rescued by nanos^{nanos.+2'A-3X.Tag:HA}

(Bergsten et al., 2001)

nanos^BN is rescued by nanos^{nanos.+2'B-3X.Tag:HA}

(Bergsten et al., 2001)

nanos^BN is rescued by nanos^{nanos.+2'D-3X.Tag:HA}

(Bergsten et al., 2001)

nanos^BN is rescued by nanos^{nanos.+2'C-3X.Tag:HA}

(Bergsten et al., 2001)

nanos^BN is rescued by nanos^{nanos.+2'ME-3X.Tag:HA}

(Bergsten et al., 2001)

nanos^BN is rescued by nanos^{nanos.+2.Hsp83}

(Crucs et al., 2000)

nanos^BN is rescued by nanos^TCE.IIIGC

(Crucs et al., 2000)

nanos^BN is rescued by nanos^{TCE.IIIA.Tloop}

(Crucs et al., 2000)

nanos^BN is rescued by nanos^TCE.IIIA:U

(Crucs et al., 2000)

nanos^BN is rescued by nanos^TCE.IIIU

(Crucs et al., 2000)

nanos^BN is rescued by nanos^TCE.IIIGC:GC

(Crucs et al., 2000)

nanos^BN is rescued by nanos^TCEIIU

(Crucs et al., 2000)

nanos^BN is rescued by nanos^TCEIIA

(Crucs et al., 2000)

nanos^BN is rescued by nanos^TCE.IIIA

(Crucs et al., 2000)

nanos^BN is rescued by nanos^ΔF

(Verrotti and Wharton, 2000)

nanos^BN is rescued by nanos^+t5.7

(Verrotti and Wharton, 2000)

nanos^BN is rescued by nanos^ΔA

(Verrotti and Wharton, 2000)

nanos^BN is rescued by nanos^ΔD

(Verrotti and Wharton, 2000)

nanos^BN is rescued by nanos^ΔB

(Verrotti and Wharton, 2000)

nanos^BN is rescued by nanos^ΔC

(Verrotti and Wharton, 2000)

nanos^BN is rescued by nanos^ΔE

(Verrotti and Wharton, 2000)

nanos^BN is rescued by nanos^{nanos.+2.Hsp83}

(Bergsten and Gavis, 1999)

nanos^BN is rescued by nanos^{nanos.+2'-3X.Tag:HA}

(Bergsten and Gavis, 1999)

nanos^BN is rescued by nanos^+4.4.Tag:HA

(Curtis et al., 1995)

Partially rescued by

nanos^BN is partially rescued by nanos^TCEIIUC.AG

(Gavis et al., 2008)

nanos^BN is partially rescued by nanos^{nanos.+2.Hsp83}

(Jain and Gavis, 2008)

nanos^BN is partially rescued by nanos^{nanos.+2'F-3X.Tag:HA}

(Bergsten et al., 2001)

nanos^BN is partially rescued by nanos^{nanos.+2'E-3X.Tag:HA}

(Bergsten et al., 2001)

nanos^BN is partially rescued by nanos^{nanos.+2RO.Tag:HA}

(Bergsten et al., 2001)

nanos^BN is partially rescued by nanos^{nanos.+2E.Tag:HA}

(Bergsten et al., 2001)

nanos^BN is partially rescued by nanos^{nanos.+2C.Tag:HA}

(Bergsten et al., 2001)

nanos^BN is partially rescued by nanos^{TCE.IIIA:U^C72}

(Crucs et al., 2000)

nanos^BN is partially rescued by nanos^{TCE.IIIA:U.Tloop}

(Crucs et al., 2000)

nanos^BN is partially rescued by nanos^{αTub84B.UTR.Tag:HA}

(Crucs et al., 2000)

nanos^BN is partially rescued by nanos^{nanos.+1.Hsp83}

(Crucs et al., 2000)

nanos^BN is partially rescued by nanos^TCEIII.TLoop

(Crucs et al., 2000)

nanos^BN is partially rescued by nanos^{nanos.+2.U51A}

(Crucs et al., 2000)

nanos^BN is partially rescued by nanos^{nanos.+2.IIA:U}

(Crucs et al., 2000)

nanos^BN is partially rescued by nanos^TCEIII.U51A

(Crucs et al., 2000)

nanos^BN is partially rescued by nanos^ΔG

(Verrotti and Wharton, 2000)

nanos^BN is partially rescued by nanos^{nanos.+2'.Hsp83}

(Bergsten and Gavis, 1999)

nanos^BN is partially rescued by nanos^{nanos.+4.Hsp83}

(Bergsten and Gavis, 1999)

nanos^BN is partially rescued by nanos^{nanos.+4-3X.Tag:HA}

(Bergsten and Gavis, 1999)

nanos^BN is partially rescued by nanos^{nanos.+3-2X.Tag:HA}

(Bergsten and Gavis, 1999)

nanos^BN is partially rescued by nanos^{nanos.+2-3X.Tag:HA}

(Bergsten and Gavis, 1999)

nanos^BN is partially rescued by nanos^{nanos.+4-2X.Tag:HA}

(Bergsten and Gavis, 1999)

nanos^BN is partially rescued by nanos^{nanos.+3.Hsp83}

(Bergsten and Gavis, 1999)

nanos^BN is partially rescued by nanos^{nanos.+2-2X.Tag:HA}

(Bergsten and Gavis, 1999)

nanos^BN is partially rescued by nanos^{nanos.+3-3X.Tag:HA}

(Bergsten and Gavis, 1999)

nanos^BN is partially rescued by nanos^{nanos.+2'-2X.Tag:HA}

(Bergsten and Gavis, 1999)

Not rescued by

nanos^BN is not rescued by nanos^{nanos.+2A.Tag:HA}

(Bergsten et al., 2001)

nanos^BN is not rescued by nanos^{TCE.ΔProxIII}

(Crucs et al., 2000)

nanos^BN is not rescued by nanos^{nanos.IIA:U+U51A}

(Crucs et al., 2000)

nanos^BN is not rescued by nanos^{αTub84B.UTR.virTCE.Tag:HA}

(Crucs et al., 2000)

nanos^BN is not rescued by nanos^TCEIIA.U

(Crucs et al., 2000)

nanos^BN is not rescued by nanos^{TCE.II^III.Tag:HA}

(Crucs et al., 2000)

nanos^BN is not rescued by nanos^{nanos.+2.IIA:U+U51A}

(Crucs et al., 2000)

Comments

wah^+t9.9 rescues the reduced abdominal segments phenotype seen in embryos produced by nos^ΔBX, nos^BN/nos^BN females that are also heterozygous for wah¹.

(Yu et al., 2010)

One copy of P{Dmnos} is sufficient to completely rescue the nos^L7 abdominal segmentation phenotype.

(Curtis et al., 1995)

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

95110

w^*; PBac{αTub67C-bcd-LEXY(FRT.bcd3'UTR)sqh3'UTR}VK00033 bcd⁶ nanos^BN e¹ tsl⁴/TM3, Sb¹

95109

w^*; PBac{αTub67C-iRFP713-bcd-LEXY(FRT.bcd3'UTR)sqh3'UTR}VK00033 bcd⁶ nanos^BN e¹ tsl⁴/TM3, Sb¹

Notes on Origin

Discoverer

Comments

Cytoplasm from the posterior pole of D.pseudoobscura.pseudoobscura, D.virilis, D.hydei, M.domestica and C.samoensis embryos is injected into the abdominal region of homozygous early cleavage stage embryos. Injected embryos displayed partial or complete abdominal segmentation, the frequency of strong rescue decreases with increasing evolutionary distance of the donor.

(Curtis et al., 1995)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

nanos^BN

nos^bn

(Seydoux and Dunn, 1997)

Name Synonyms

Secondary FlyBase IDs

References (60)

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