FlyBase Allele Report: Dmel\nanos[+1+3.Tag:MS2]

General Information

Symbol

Dmel\nanos^+1+3.Tag:MS2

Species

D. melanogaster

Name

FlyBase ID

FBal0248902

Feature type

allele

Associated gene

Dmel\nanos

Associated Insertion(s)

Carried in Construct

P{nanos+1+3}

Key Links

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Becalska et al., 2011)

Progenitor genotype

Carried in construct

P{nanos+1+3}

Cytology

Description

nos 5' UTR and coding region, followed by the sensitised 3' UTR containing nucleotides 6-96 (the +1 localisation element sequence), 181-408 (the +3 localisation element sequence), and 548-786 of the nos 3' UTR. Tag:MS2 stem loops have been inserted near the terminus of the 3' UTR.

(Becalska et al., 2011)

Allele components

Component

Use(s)

Encoded product / tool

nanos

Tagged with

Tag:MS2

specific binding tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

abdominal segment, with nanos^BN

(Becalska et al., 2011)

Detailed Description

Statement

Reference

nos^BN,nos^{+1+3.T:MS2\MCP.BS}/nos^BN embryos display a reduction in abdominal segmentation, forming an average of 6 of the 8 abdominal segments characteristic of wild-type embryos.

(Becalska et al., 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhanced by

Statement

Reference

nanos^+1+3.Tag:MS2, nanos^BN has abdominal segment phenotype, enhanceable by Df(2L)BSC32/+

(Becalska et al., 2011)

nanos^+1+3.Tag:MS2, nanos^BN has abdominal segment phenotype, enhanceable by aub[+]/aub^QC42

(Becalska et al., 2011)

nanos^+1+3.Tag:MS2, nanos^BN has abdominal segment phenotype, enhanceable by aub[+]/aub^HN2

(Becalska et al., 2011)

NOT Enhanced by

Statement

Reference

nanos^+1+3.Tag:MS2, nanos^BN has abdominal segment phenotype, non-enhanceable by squ[+]/squ^HE47

(Becalska et al., 2011)

Enhancer of

Statement

Reference

nanos^BN/nanos^+1+3.Tag:MS2 is an enhancer of abdominal segment phenotype of rump¹

(Becalska et al., 2011)

nanos^BN/nanos^+1+3.Tag:MS2 is an enhancer of abdominal segment phenotype of aub[+]/aub^QC42, rump¹

(Becalska et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

nos^BN,nos^{+1+3.T:MS2\MCP.BS}/nos^BN embryos carrying a Df(2L)BSC32/+ mutation display a reduction in abdominal segmentation, forming an average of 2.4 abdominal segments.

nos^BN,nos^{+1+3.T:MS2\MCP.BS}/nos^BN embryos carrying a aub^QC42/+ mutation display a reduction in abdominal segmentation, forming an average of 2-3 abdominal segments. Mutant embryos display the normal number of pole cells.

nos^BN,nos^{+1+3.T:MS2\MCP.BS}/nos^BN embryos carrying a aub^HN/+ mutation display a reduction in abdominal segmentation, forming an average of 3-4 abdominal segments.

nos^BN,nos^{+1+3.T:MS2\MCP.BS}/nos^BN embryos carrying a squ^HE47/+ mutation do not display a reduction in abdominal segmentation compared to nos^BN,nos^{+1+3.T:MS2\MCP.BS}/nos^BN embryos.

rump¹ mutant embryos carrying a nos^BN, nos^{+1+3.T:MS2\MCP.BS}/nos^BN mutation display an increase in the number of embryos that develop less than 8 abdominal segments.

rump¹ mutant embryos carrying an aub^QC42/+ mutation, and the mutation nos^BN, nos^{+1+3.T:MS2\MCP.BS}/nos^BN , display an increase in the number of embryos that develop less than 8 abdominal segments.

(Becalska et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

nanos^+1+3.Tag:MS2

nos^{+1+3.T:MS2\MCP.BS}

nos^{+1+3.Tag:BS(MS2-CP)}

nos^+1+3.Tag:MS2

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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