Three copies of a mutated +2' element from the nos 3' UTR are inserted within the αTub84B 3' UTR sequences of nosαTub84B.UTR.Tag:HA.
Embryos derived from nosBN females which are also expressing nosnos.+2'E-3X.T:Ivir\HA1 do not have anterior defects.
nanosnanos.+2'E-3X.Tag:HA partially rescues nanosBN
nosnos.+2'E-3X.T:Ivir\HA1 partially rescues the abdominal segmentation defect of embryos derived from nosBN females; 28% of the embryos develop 1-3 abdominal segments, 67% develop 4-6 abdominal segments and 5% develop 7-8 abdominal segments.