Genomic nanos fragment in which the nanos 3'UTR has been replaced with the αTub84B 3'UTR. Three direct repeats of the +2' element from the nanos 3' untranslated region (UTR) have been inserted in the αTub84B 3' UTR sequences. A Tag:HA epitope has been inserted at the 5' end of the nanos coding region.
Embryos derived from vas1/vas3 females carrying nosnos.+2'-3X.T:Ivir\HA1 develop 0-3 abdominal segments in 72% of cases and 4-6 abdominal segments in 28% of cases.
98% of embryos derived from nosBN females carrying nosnos.+2'-3X.T:Ivir\HA1 develop 7-8 abdominal segments.