One copy of a +2 element from the nos 3' UTR with a mutation in the +2' region is inserted within the αTub84B 3' UTR sequences of nosαTub84B.UTR.Tag:HA.
Embryos derived from nosBN females which are also expressing nosnos.+2A.T:Ivir\HA1 do not have anterior defects.
nanosnanos.+2A.Tag:HA fails to rescue nanosBN
nosnos.+2A.T:Ivir\HA1 fails to rescue the abdominal segmentation defect of embryos derived from nosBN females; 92% of the embryos develop no abdominal segments and 8% develop 1-3 abdominal segments.