Stem-loops II and III of the nos TCE sequences, separated by a 52bp spacer, have been inserted into the αTub84B 3' UTR sequences.
nanosTCE.II^III.Tag:HA fails to rescue nanosBN
nosTCE.II^III.T:Ivir\HA1 fails to rescue the abdominal segmentation defect of embryos derived from nosBN females.