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General Information
Symbol
Dmel\nosL7
Species
D. melanogaster
Name
FlyBase ID
FBal0013153
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
deletion
Comment:

Deletion of seven codons (ITMEDAI).

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Deletes nucleotides 2009-2029, inclusive, eliminating seven codons (ITMEDAI). These seven residues fall just outside the second CCHC domain.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Embryos derived from homozygous females have less than 3 abdominal segments.

Embryos form a wildtype number of germ cells at blastoderm but not all of theses germ cells enter the mutant embryos. Cells that remain outside degenerate.

Germ cells are present in embryos derived from homozygous females.

Has no effect on the posterior accumulation of G-iα65A protein.

bcd, nos double mutants show reduced rate of germ band extension and cell intercalation.

Embryos from homozygous mothers produce extra pole cells at the posterior pole.

Embryos derived from homozygous mothers lack abdominal cuticle. 22% of embryos carrying nubhs.PC and derived from homozygous mothers produce 2-3 abdominal denticle belts when heat shocked at 1.75 to 2.75 hours of development. 4% of embryos carrying pdm2hs.PC and derived from homozygous mothers produce 2-3 abdominal denticle belts when heat shocked at 1.75 to 2.75 hours of development.

Embryos carrying six copies of osk (6xP{osk+108}) examined at the cellular blastoderm stage exhibit ectopic pole cell formation, this ectopic formation is enhanced in a nosL7 mutant background.

Uniform distribution of maternal hb protein. The posterior gt stripe fails to appear, while the anterior pattern appears normal.

Strong abdominal defects.

Posterior gt domain is usually absent.

Does not interact with RpII140wimp maternal effect.

Homozygous embryos lack all abdominal segments. Extreme embryos exhibit a bic phenotype.

Large amounts of vas protein are expressed in early stages of oogenesis. vas protein localizes to the posterior pole at the same stage of egg development as wild type.

nosL7/nos53 transheterozygous embryos derived from homozygous females have polar granules and pole cells and have deletions of abdominal structures. CycB and vas transcript localization and accumulation is as in wild type.

Abdominal segments deleted, head, thorax and telson are normal. Posterior transplantation of anterior cytoplasm from wild type embryos induced high rate of anterior structure duplication.

No abdominal segmentation is formed but head, thorax and telson are normal. Injection of wild type posterior pole plasm induces formation of an almost complete set of abdominal segments in normal anteroposterior orientation.

All abdominal segments are deleted in embryos derived from homozygous females.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Suppressed by
Statement
Reference

hb4, nosL7 has tergite phenotype, suppressible by mxcG48

nosL7 has phenotype, suppressible by Dvir\nostCa

Suppressor of
NOT Suppressor of
Statement
Reference

nosL7/nos[+] is a non-suppressor of ovary phenotype of l(3)mbtGM76/Df(3R)ED10966

nosL7/nos[+] is a non-suppressor of ovariole phenotype of l(3)mbtGM76/Df(3R)ED10966

nosL7/nos[+] is a non-suppressor of egg chamber phenotype of l(3)mbtGM76/Df(3R)ED10966

nosL7/nos[+] is a non-suppressor of nurse cell | increased number phenotype of l(3)mbtGM76/Df(3R)ED10966

nosL7/nos[+] is a non-suppressor of oocyte | increased number phenotype of l(3)mbtGM76/Df(3R)ED10966

nosL7/nos[+] is a non-suppressor of follicle cell phenotype of l(3)mbtGM76/Df(3R)ED10966

Other
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference

nosL7/Df(3R)Exel6183 partially suppresses the wing disc posterior compartment overgrowth of larvae expressing ykiScer\UAS.cHa under the control of Scer\GAL4en.PU.

Expression of either E2f2JF02828 or mip130HMS00462 under the control of Scer\GAL4nub.PU (using UAS-Dicer2 to enhance RNAi efficiency) in the nosL7 mutant background results in adult wing defects (minor ectopic venation). No wing phenotype is observed upon expression of either RbfHMS03004 or mip120HMS00461 in the nosL7 mutants.

Anisotropic epithelial constriction still occurs in bcd6 nosL7 tsl3 triple mutant embryos that lack anterior-posterior polarity.

wah1, nosBN/nosL7 females do not exhibit age-dependent loss of fecundity.

Anterior-posterior polarization of cells in the extending germband is disrupted in embryos from bcd6 nosL7 tsl3 homozygous mothers.

3.69% of embryos derived from hb4 nosL7/nosL7 females have at least 3 abdominal segments.

Embryos derived from bcd6 nosL7 tsl3 triple mutant females have uniform yolk stalk diameters (in contrast to wild type where there are three domains of varying yolk stalk diameter along the anterior-posterior axis of the embryo). The shallow cellularisation front of the anterior domain and the greater depth of the pre-cephalic furrow domain are also lost. Other aspects of cellularisation are normal in these embryos. Nuclear spacing is uniform along the anterior-posterior axis in embryos derived from bcd6 nosL7 tsl3 triple mutant females (in contrast to wild type where there is an anterior domain of lower nuclear density). The larger diameter of the actin caps seen in the anterior of the wild-type embryo in cycle 11 and 12 is not seen in embryos derived from bcd6 nosL7 tsl3 triple mutant females.

nosL7 homozygous embryos derived from nosL7 hb15 homozygous female germline clones form germ cells normally, but show defects after stage 10 of embryogenesis. At this stage, many germ cells fail to leave the gut (in contrast to wild type) and tightly associate into clusters of cells. Some germ cells follow a normal migratory pattern, the morphology of the germ cells is normal and the embryos develop into fertile flies.

Embryos derived from hb4 nosL7/nosL7 females differentiate only a few abdominal denticle belts. No rescue of abdominal segments is seen if the females also carry Df(1)10-70d, Df(1)mxc-1, mxc22a-6 or mxc1. Rescue of abdominal segments in embryos derived from hb4 nosL7/nosL7 females is seen if the females are also carrying mxcG48. Adult escapers have abnormal tergites and are mostly fertile. Rescue of abdominal segments in embryos derived from hb4 nosL7/nosL7 females is seen if the females are also carrying Df(2R)vg-B or phob.

bcd6 nosL7 embryos exhibit two telsons in mirror image. Injection of wild type posterior pole plasm induces formation of two mirror image abdomens. bcd6 nosL7 tll1 embryos exhibit two telsons in mirror image. Injection of wild type posterior pole plasm induces formation of two mirror image abdomens. bcd6 nosL7 tsl3 embryos develop a cuticle but no segmental pattern. Injection of wild type posterior pole plasm induces formation of anterior abdominal segments towards the end of the embryo.

Xenogenetic Interactions
Statement
Reference

Two copies of P{Dvnos} partially rescues the mutant phenotype.

Complementation and Rescue Data
Comments

One copy of P{Dmnos} is sufficient to completely rescue the nosL7 abdominal segmentation phenotype.

Abdominal segmentation defect can be rescued by injection of nosN5 RNA before pole cell formation, embryos hatch and develop into fertile adults. Injection into the head region causes an anterior shift in the postion of the cephalic furrow and reduction of head skeletal structures.

Images (0)
Mutant
Wild-type
Stocks (5)
Notes on Origin
Discoverer

Lehmann.

Comments
Comments

hb protein expression in early nosL7 mutant embryos has been studied.

Posterior cytoplasm from embryos derived from nosL7 females can rescue both anterior and posterior defects when transplanted into the anterior or posterior end respectively of embryos derived from tor4 females.

Pole plasm is present and functional in germ cell determination. Cytoplasmic transplantation of wild type pole plasm into the abdominal region restores normal abdominal development: surviving adults are fertile.

The posterior localization of tud product is unaffected in mutant embryos.

Cytoplasm from the posterior pole of D.pseudoobscura.pseudoobscura, D.virilis, D.hydei, M.domestica and C.samoensis embryos is injected into the abdominal region of homozygous early cleavage stage embryos. Injected embryos displayed partial or complete abdominal segmentation, the frequency of strong rescue decreases with increasing evolutionary distance of the donor.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
References (69)