57% of Hsp8308445/Hsp83e6A flies show a loss of locomotor behavioral rhythms under free-running conditions (after entrainment in light-dark cycles).
Hsp8308445/Hsp83e6A flies show a reduced fraction of flies with rhythmic locomotor activity in light-dark cycles (50% rhythmic) compared to control flies (88%-95% rhythmic).
Hsp8308445/Hsp83e6A mutants show highly variable locomotor activity patterns, including fully rhythmic, arrhythmic, and complex behavioral profiles, in contrast to wild-type flies, which show little variation among individuals.
Hsp8319F2/Hsp83e6A females have a series of ovarian defects. Approximately 75% of egg chambers are blocked at different developmental stages, not later than stage 9. The remaining egg chambers show a pronounced defect in the transfer of nurse cell cytoplasm to the oocyte; the nuclei of the nurse cells do not degenerate at the end of the dumping process, as occurs in wild-type egg chambers, but persist up to later stages of oogenesis. Mutant mature eggs are smaller in size than normal and have altered dorsal appendages.
Hsp83e6A flies show normal wing positions.
Hsp83e6A has no effect on an invariant bristle trait (thoracic and scutellar bristles were analysed). Hsp83e6A has a significant effect on a variable bristle trait (the sternopleural, orbital, ocellar and vibrissa and carina bristles were analysed); there is a significant difference in trait mean compared to the background strain in which the Hsp83e6A mutation was induced.
Viable in transheterozygous combination with Hsp839J1; males are sterile and females are weakly fertile. Viable in transheterozygous combination with Hsp8313F3; males and females are sterile. Hsp839J1/Hsp83e6A males show defects during spermatogenesis. The number and shape of spermatocytes within 16-cell cysts are mostly normal (5-10% are abnormal). Spermatids with variable number, size and shape of nuclei and nebenkern are seen. Needle-shaped crystals are present throughout developing spermatocytes and spermatids. Individualised sperm are present but they are not motile and are fragile. Hsp8308445/Hsp83e6A males show defects during spermatogenesis. Excessive numbers of primary spermatocytes are seen in many developing cysts. Spermatids with variable number, size and shape of nuclei and nebenkern are seen. Needle-shaped crystals are present throughout developing spermatocytes and spermatids. Individualised sperm are present but they are not motile and are fragile.