FlyBase Allele Report: Dmel\Hsp83[e4A]

General Information

Symbol

Dmel\Hsp83^e4A

Species

D. melanogaster

Name

FlyBase ID

FBal0029643

Feature type

allele

Associated gene

Dmel\Hsp83

Associated Insertion(s)

Carried in Construct

Genomic Maps

GBrowse JBrowse

Help me choose

Allele class

hypomorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(van der Straten et al., 1997)

Mutagen

ethyl methanesulfonate

(van der Straten et al., 1997)

Mutations Mapped to the Genome

Type

Location

Additional Notes

References

point mutation

3L:3,196,462..3,196,462 [+]

Nucleotide change:

C3196462T

Reported nucleotide change:

C1964T

Amino acid change:

S655F | Hsp83-PA; S655F | Hsp83-PB

Reported amino acid change:

S655F

(Cutforth and Rubin, 1994)

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Progenitor genotype

Cytology

Nature of the lesion

Statement

Reference

Amino acid replacement: S655F. Nucleotide substitution: C1964T.

(van der Straten et al., 1997)

Nucleotide substitution: C1964T. Amino acid replacement: S655F.

(Cutforth and Rubin, 1994)

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Phenotypic Data

Phenotypic Class

lethal | recessive

(Yue et al., 1999, Simon et al., 1991)

male sterile (with Hsp83^6-55)

(Bandura et al., 2013)

Phenotype Manifest In

Nebenkern

(Yue et al., 1999)

primary spermatocyte cyst

(Yue et al., 1999)

spermatid

(Yue et al., 1999)

spermatocyte

(Yue et al., 1999)

spermatozoon

(Yue et al., 1999)

Detailed Description

Statement

Reference

Viable in transheterozygous combination with Hsp83^9J1; males are sterile and females are weakly fertile. Hsp83^9J1/Hsp83^e4A males show some defects during spermatogenesis. The number and shape of spermatocytes within 16-cell cysts are mostly normal (5-10% are abnormal). The nuclei and nebenkern within spermatids are mostly normal (5-10% are abnormal). Individualised sperm are present, but are not motile. Hsp83⁰⁸⁴⁴⁵/Hsp83^e4A males show defects during spermatogenesis. Excessive numbers of primary spermatocytes are seen in many developing cysts. Spermatids with variable number, size and shape of nuclei and nebenkern are seen. Individualised sperm are present but they are not motile and are fragile.

(Yue et al., 1999)

Mutation fails to genetically interact with sev^{S11.T:Hsap\MYC}, 'sev³⁵¹', phl¹² or phl::tor^12D.hs.sev.

(van der Straten et al., 1997)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Other

Statement

Reference

Hsp83^e4A/Hsp83[+], Kr^If-1 has eye phenotype

(Sollars et al., 2003)

Hsp83^e4A, Kr^If-1 has eye phenotype

(Sollars et al., 2003)

Additional Comments

Genetic Interactions

Statement

Reference

6+/-2% of Kr^If-1/+ flies born to Hsp83^e4A/+ mothers have outgrowths with ectopic vibrissae protruding from the ventral region of the eye, compared to less than 0.1% of Kr^If-1/+ flies born to isogenised wild-type mothers.

(Sollars et al., 2003)

Enhances the sev^B4 phenotype: disrupts sev^B4 protein signalling.

(Simon et al., 1991)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Complements

Hsp83^9J1

(van der Straten et al., 1997)

Partially complements

Hsp83⁰⁸⁴⁴⁵

(Yue et al., 1999)

Fails to complement

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

E(sev)3A^e4A

Hsap83^S655F

(Kimura et al., 1997)

Hsp83^e4A

(Bandura et al., 2013)

S655F

(Yue et al., 1999)

e4A

(Bandura et al., 2013)

hsp83^e4A

(Specchia et al., 2010)

Name Synonyms

Secondary FlyBase IDs

References (8)

Report Sections

Open Close