FlyBase Allele Report: Dmel\Hsp83[e1D]

General Information

Symbol

Dmel\Hsp83^e1D

Species

D. melanogaster

Name

FlyBase ID

FBal0029641

Feature type

allele

Associated gene

Dmel\Hsp83

Associated Insertion(s)

Carried in Construct

Also Known As

e1D

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(van der Straten et al., 1997)

Mutagen

ethyl methanesulfonate

(van der Straten et al., 1997)

Progenitor genotype

Cytology

Description

Amino acid replacement: S38L. Nucleotide substitution: C113T.

(van der Straten et al., 1997)

Nucleotide substitution: C113T. Amino acid replacement: S38L.

(Cutforth and Rubin, 1994)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3L:3,194,611..3,194,611 [+]

Nucleotide change:

C3194611T

Reported nucleotide change:

C113T

Amino acid change:

S38L | Hsp83-PA; S38L | Hsp83-PB

Reported amino acid change:

S38L

(Cutforth and Rubin, 1994)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal | recessive

(Yue et al., 1999, Simon et al., 1991)

male sterile (with Hsp83^6-55)

(Bandura et al., 2013)

viable (with Hsp83^9J1)

(Milton et al., 2005)

viable (with Hsp83^13F3)

(Milton et al., 2005)

viable (with Hsp83^19F2)

(Milton et al., 2005)

Phenotype Manifest In

chaeta

(Milton et al., 2005)

(Yue et al., 1999)

(Yue et al., 1999)

(Yue et al., 1999)

(Yue et al., 1999)

Detailed Description

Statement

Reference

Hsp83^e1D has no effect on an invariant bristle trait (thoracic and scutellar bristles were analysed). Hsp83^e1D has a significant effect on a variable bristle trait (the sternopleural, orbital, ocellar and vibrissa and carina bristles were analysed); there is a significant difference in trait mean compared to the background strain in which the Hsp83^e1D mutation was induced.

(Milton et al., 2005)

Viable in transheterozygous combination with either Hsp83^13F3 or Hsp83^9J1; males are sterile and females are weakly fertile. Hsp83^e1D/Hsp83^13F3 and Hsp83^e1D/Hsp83^9J1 males show defects during spermatogenesis. The number and shape of spermatocytes within 16-cell cysts are mostly normal (5-10% are abnormal). Spermatids with variable number, size and shape of nuclei and nebenkern are seen. Needle-shaped crystals are present throughout developing spermatocytes and spermatids. Individualised sperm are present but they are not motile and are fragile.

(Yue et al., 1999)

Mutation fails to genetically interact with sev^{S11.T:Hsap\MYC}, 'sev³⁵¹', phl¹² or phl::tor^12D.hs.sev.

(van der Straten et al., 1997)

No effect the rough eye phenotype (formation of extra R7 cells) caused by P{sev-svp2} and no effect on the P{ro-svp1} rough eye phenotype (loss of one or more outer photoreceptors from many of the ommatidia).

(Kramer et al., 1995)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

15+/-4% of Kr^If-1/+ flies born to Hsp83^e1D/+ mothers have outgrowths with ectopic vibrissae protruding from the ventral region of the eye, compared to less than 0.1% of Kr^If-1/+ flies born to isogenised wild-type mothers.

(Sollars et al., 2003)

Enhances the sev^B4 phenotype: disrupts sev^B4 protein signalling.

(Simon et al., 1991)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Complements

Hsp83^9J1

(van der Straten et al., 1997)

Hsp83^13F3

(van der Straten et al., 1997)

Fails to complement

Rescued by

Hsp83^e1D/Hsp83^e6A is rescued by Hsp83^GFP

(Tariq et al., 2009)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

No interaction with P{sev-svp1} or P{sev-svp2} exists.

(Begemann et al., 1995)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

E(sev)3A^e1D

Hsap83^S38L

(Kimura et al., 1997)

Hsp83^e1D

(Bandura et al., 2013)

S38L

(Yue et al., 1999)

e1D

(Tariq et al., 2009, Rutherford and Lindquist, 1998)

elD

(Bandura et al., 2013)

Name Synonyms

Secondary FlyBase IDs

References (12)

Report Sections

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