FlyBase Allele Report: Dmel\cup[21]

General Information

Symbol

Dmel\cup²¹

Species

D. melanogaster

Name

FlyBase ID

FBal0002166

Feature type

allele

Associated gene

Dmel\cup

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Keyes and Spradling, 1997, Schupbach and Wieschaus, 1991)

Progenitor genotype

Cytology

Description

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal mitotic cell cycle | oogenesis stage S5

(Sugimura and Lilly, 2006)

female sterile

(Schupbach and Wieschaus, 1991)

female sterile | partially | recessive

(Keyes and Spradling, 1997)

Phenotype Manifest In

cleavage nucleus

(Keyes and Spradling, 1997)

egg chamber

(Zappavigna et al., 2004, Keyes and Spradling, 1997)

nurse cell & chromatin

(Keyes and Spradling, 1997)

nurse cell | oogenesis stage S5

(Sugimura and Lilly, 2006)

ovary

(Zappavigna et al., 2004)

Detailed Description

Statement

Reference

In cup²¹ homozygous adult females, a fraction of nurse cells condense their chromosomes and enter an ectopic mitosis after stage 5 of oogenesis.

(Sugimura and Lilly, 2006)

Egg chambers arrest at stage S14. Nurse cell nuclear chromatin in the oldest egg chambers shows variable condensation. Flies are weakly fertile. The small fraction of embryos derived from homozygous females that do develop have condensed, irregularly spaced nuclei at the cleavage stage.

(Keyes and Spradling, 1997)

Abnormalities in follicle cell migration: open ended chorion.

(Schupbach and Wieschaus, 1991)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhanced by

Statement

Reference

cup²¹ has egg chamber phenotype, enhanceable by mira[+]/mira^AB78

(Piccioni et al., 2009)

cup²¹ has egg chamber phenotype, enhanceable by mira^JJ92/mira[+]

(Piccioni et al., 2009)

cup²¹ has egg chamber phenotype, enhanceable by mira^L44/mira[+]

(Piccioni et al., 2009)

cup²¹ has egg chamber phenotype, enhanceable by mira^YY227/mira[+]

(Piccioni et al., 2009)

cup²¹ has egg chamber phenotype, enhanceable by Hsp83^19F2/Hsp83[+]

(Pisa et al., 2009)

cup²¹ has egg chamber phenotype, enhanceable by Hsp83[+]/Hsp83^e6A

(Pisa et al., 2009)

cup²¹ has egg chamber phenotype, enhanceable by eIF4E1⁰⁷²³⁸/eIF-4E[+]

(Zappavigna et al., 2004)

cup²¹ has ovary phenotype, enhanceable by eIF4E1⁰⁷²³⁸/eIF-4E[+]

(Zappavigna et al., 2004)

NOT Enhanced by

Statement

Reference

cup²¹ has nurse cell & chromosome phenotype, non-enhanceable by eIF4E1⁰⁷²³⁸/eIF-4E[+]

(Zappavigna et al., 2004)

Suppressor of

Statement

Reference

cup²¹/cup[+] is a suppressor of egg chamber phenotype of nanos¹⁸

(Verrotti and Wharton, 2000)

cup²¹/cup[+] is a suppressor of ovary phenotype of nanos¹⁸

(Verrotti and Wharton, 2000)

Other

Statement

Reference

Hsp83^19F2/Hsp83[+], cup²¹ has egg chamber phenotype

(Pisa et al., 2009)

Hsp83[+]/Hsp83^e6A, cup²¹ has egg chamber phenotype

(Pisa et al., 2009)

Nup154^tlp1, cup²¹ has ovariole phenotype

(Grimaldi et al., 2007)

cup²¹, nanos¹⁸ has embryonic abdominal segment phenotype

(Verrotti and Wharton, 2000)

cup²¹/cup[+], nanos¹⁸ has embryonic abdominal segment phenotype

(Verrotti and Wharton, 2000)

Additional Comments

Genetic Interactions

Statement

Reference

The ovarian phenotype of cup²¹ homozygous females is enhanced if they are also heterozygous for one of mira^AB78, mira^JJ92, mira^L44 or mira^YY227; a greater fraction of egg chambers arrest development between stages 3 and 5 in the double mutant ovaries compared to in the single mutant ovaries.

(Piccioni et al., 2009)

The oogenesis defects seen in cup²¹ females are enhanced if they are also heterozygous for Hsp83^19F2 or Hsp83^e6A; double mutants show earlier egg chamber growth arrest and precocious degeneration of the nurse cell nuclei. The double mutant egg chambers are often misshapen, with egg chambers either unbudded or fused (seen by the presence of supernumerary nurse cell nuclei in the egg chamber).

(Pisa et al., 2009)

The size of the ovaries in cup²¹ homozygous adults is reduced by eIF-4E⁰⁷²³⁸/+. Chromosome morphology in the nurse cells of these ovaries is unaffected by eIF-4E⁰⁷²³⁸/+, but oogenesis is blocked at an earlier stage (around stage 6).

(Zappavigna et al., 2004)

The oogenesis defects seen in hemizygous nos¹⁸ females are substantially suppressed by one copy of cup²¹. Many egg chambers in the rescued ovaries appear normal and mature into oocytes that are fertilised and oviposited (the resulting embryos develop no abdominal segments). The rescued females lay eggs for at least 3 weeks.

(Verrotti and Wharton, 2000)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

29719

y¹; cup²¹ cn¹ bw¹/CyO

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

cup²¹

(Piccioni et al., 2009, Pisa et al., 2009, Grimaldi et al., 2007, Sugimura and Lilly, 2006)

cup^QM66

Name Synonyms

Secondary FlyBase IDs

References (9)

Report Sections

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