FlyBase Allele Report: Dmel\nanos[18]

nos¹⁸/nos⁵³ transheterozygote third instar larvae show dendritic field coverage defects and loss of terminal branches in class IV dendritic arborizing neurons.

(Bhogal et al., 2016)

The majority of germaria in nos¹⁸/Df(3R)Exel6183 females produce gametes but become progressively agametic over time.

(Chau et al., 2012)

Females carrying nos¹⁸/Df(3R)nos alleles display ovaries that are largely agametic.

(Li et al., 2009)

nos¹⁸/Df(3R)Dl-FX1 third instar larvae have a significantly higher total number of boutons at the neuromuscular junction (muscle 6/7), compared to controls; the number of active zones in type 1s boutons is also significantly increased.

(Menon et al., 2009)

From the first larval instar through the early third instar stage, class IV da neurons in nos¹⁸/Df(3R)Dl-X43 animals show no significant difference in dendrite branching complexity compared to those in control animals. Morphological defects in the class IV da neurons are first detected in nos¹⁸/Df(3R)Dl-X43 animals at the late third instar stage, when a significant reduction of higher order branching is seen compared to controls (there is a dramatic decrease in the density of terminal dendritic branches).

Late third instar nos¹⁸/nos⁵³ larvae show a significant reduction in the density of terminal dendritic branches of the class IV da neurons.

(Brechbiel and Gavis, 2008)

nos¹⁸/+ females have a normal number of germline stem cells per germarium.

(Maines et al., 2007)

nos¹⁸/Df(3R)Dl-FX3 gonads in larvae at the larval/pupal transition contain many developed cysts. Homozygous germ line stem cell (GSC) clones are rapidly lost from the adult ovary; GSC loss is seen as early as 4 days after clone induction, and by the 6th or 7th day after clone induction, most ovarioles do not contain a mutant GSC.

(Gilboa and Lehmann, 2004)

Third larval instar class I or class II dendritic arborization neurons which are mutant for nos¹⁸ (generated using the MARCM technique) do not show an alteration in dendrite morphology, as assessed by total length of dendrites and quantitation of dendritic order. Third larval instar class III dendritic arborization neurons which are mutant for nos¹⁸ (generated using the MARCM technique) have significantly elongated dendritic spikes, but the order of dendrites and the length of major dendritic branches is indistinguishable from wild type. Third larval instar class IV dendritic arborization neurons which are mutant for nos¹⁸ (generated using the MARCM technique) show a defect in their dendrites; incomplete coverage of the epidermis is seen in about 20% of the mutant neurons (wild-type neurons cover the epidermis in a complete but non-overlapping fashion), due to a reduction of higher-order branches.

(Ye et al., 2004)

Homozygous females lay only a few eggs (an average of 6.6 eggs per week compared to 185.0 per week for control females).

(Mochizuki et al., 2000)

Hemizygous ovaries contain only rare mature egg chambers.

(Verrotti and Wharton, 2000)

Ovaries of hemizygous females or nos¹⁸/nos⁵³ transheterozygotes contain ovarioles with either no egg chambers or 1-7 egg chambers of different stages. Some of the ovarioles produce only one egg. Ovaries with no developing germ cells occur. Mutant females show three types of egg laying pattern. TypeI (50% hemizygotes): Egg deposition begins on day 2 and stops by day 5. TypeII (41% hemizygotes): Egg deposition begins on day 2 and stops around day 5, to resume after 2 to 12 days. Type III (9% hemizygotes): No eggs are laid (ovarioles devoid of germ cells). Germline defects are age-dependent. Germaria eventually lose stem cells. Plasma membrane degenerates in stem cells and their descendents. The nuclear membrane is unaffected. Germaria from older females contain an abnormally high number of mitochondria. 20% of hemizygous or nos¹⁸/nos⁵³ males show morphologically distinct testis defects: fewer sperm bundles with a large pool of primary spermatocytes filling most of the anterior region of the testis. Number of primary spermatocytes is increased threefold (for hemizygote) compared to wild type. The defective testis generally has fewer coils than wild type. Mutant males can be sorted into three types with respect to ability to inseminate females. TypeIII males are unable to inseminate females throughout a 4 week test period (5% of hemizygous males - testis always have germ cells, either undifferentiated primary spermatocytes, or apparently defective sperm). 90% of males show a Type II defect, 5% show a type 1 defect - all males are sterile by the third week.

(Bhat, 1999)

The effects of loss of maternal and zygotic nos product on germ cell migration is studied in females with hb¹⁵ nos^BN mutant germ line clones crossed to nos¹⁸/Df(3R)Dl-FX3 males. Germ cells are formed in the embryos indicating nos function is not required for their formation. nos activity is essential for germ cell migration, from stage 10 onwards. Following exit of the germ cells of the posterior midgut pocket, germ cells fail to migrate over the surface of the gut and instead cluster tightly together on the outer gut surface. Mutant germ cells are of varying size and often have an irregular surface. Zygotic nos expression cannot compensate for the loss of maternal nos. The few germ cells that appear to associate with the somatic embryonic gonad are unable to incorporate into the adult gonad even in the presence of a zygotically active copy of the gene. nos¹⁸/Df(3R)Dl-FX3 females lay a small number of eggs early in life but soon become completely sterile. Ovaries from females ranging from 1 to 40 days old are dissected and demonstrate sterility results from loss of germ cells from the ovary. Germline cysts rarely develop into egg chambers after the first few chambers have left the germarium. Putative germ line stem cells are present in the germaria of many mutants ovarioles for at least 10 days following eclosion, these have completely disappeared by 21 days. Transplantation of wild type germ cells can rescue the nos ovary phenotype. The transplanted cells populate the ovary more efficiently in embryos whose endogenous germ cells lack nos activity than in embryos with germ cells containing a zygotically active copy of nos.

(Forbes and Lehmann, 1998)

Homozygous ovaries contain fewer developing cysts than normal. Many ovarioles consist of a germarium with one to three cysts followed by an extended stalk and one to three normal looking egg chambers. In more extreme cases, only remnants of spectrosome/fusome material can be detected in the anterior of the germarium.

(Styhler et al., 1998)

Abdominal segmentation phenotype.

(Curtis et al., 1995)

Homozygous embryos show mirror image duplications of posterior abdominal structures and telson structures. Defect in oogenesis so females only produce small numbers of eggs, presence of the P{nos-bcd3'UTR} construct does not rescue this defect.

(Gavis and Lehmann, 1992)

maternal-effect lethal. segments, but have normal pole cells and pole plasm; no posterior activity in pole plasm. Transport or diffusion of the nos gene product from the posterior of the embryo seems to be essential for development of the wild-type abdominal pattern. Presence of the nos protein represses the activity of the gene product encoded by the hb maternal transcript in the posterior half of the embryo (Hulskamp, Schroder, Pfeifle, Jackle and Tautz, 1989; Irish, Lehmann and Akam, 1989; Struhl, 1989). Eggs deficient for both hb and nos, when fertilized by hb⁺ sperm, develop into normal embryos and subsequently into viable flies. Mutant embryos lack abdominal

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

nanos⁵³/nanos¹⁸ has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by hid^A206/hid[+]

(Bhogal et al., 2016)

nanos⁵³/nanos¹⁸ has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by Dronc^KK104278/Scer\GAL4⁴⁷⁷

(Bhogal et al., 2016)

nanos⁵³/nanos¹⁸ has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by Drice^GD12284/Scer\GAL4⁴⁷⁷

(Bhogal et al., 2016)

nanos⁵³/nanos¹⁸ has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by Dcp-1^KK106134/Scer\GAL4⁴⁷⁷

(Bhogal et al., 2016)

Phenotype Manifest In

Suppressed by

Statement

Reference

nanos⁵³/nanos¹⁸ has larval multidendritic class IV neuron | third instar larval stage phenotype, suppressible by hid^A206/hid[+]

(Bhogal et al., 2016)

nanos⁵³/nanos¹⁸ has dendrite | third instar larval stage phenotype, suppressible by hid^A206/hid[+]

(Bhogal et al., 2016)

nanos⁵³/nanos¹⁸ has larval multidendritic class IV neuron | third instar larval stage phenotype, suppressible by Dronc^KK104278/Scer\GAL4⁴⁷⁷

(Bhogal et al., 2016)

nanos⁵³/nanos¹⁸ has dendrite | third instar larval stage phenotype, suppressible by Dronc^KK104278/Scer\GAL4⁴⁷⁷

(Bhogal et al., 2016)

nanos⁵³/nanos¹⁸ has larval multidendritic class IV neuron | third instar larval stage phenotype, suppressible by Drice^GD12284/Scer\GAL4⁴⁷⁷

(Bhogal et al., 2016)

nanos⁵³/nanos¹⁸ has larval multidendritic class IV neuron | third instar larval stage phenotype, suppressible by Dcp-1^KK106134/Scer\GAL4⁴⁷⁷

(Bhogal et al., 2016)

nanos⁵³/nanos¹⁸ has dendrite | third instar larval stage phenotype, suppressible by Dcp-1^KK106134/Scer\GAL4⁴⁷⁷

(Bhogal et al., 2016)

nanos¹⁸/Df(3R)nanos has germline stem cell phenotype, suppressible by bam^EP667/bam[+]

(Li et al., 2009)

nanos¹⁸/Df(3R)nanos has ovary phenotype, suppressible by bgcn[+]/bgcn^bgcnP.GFP

(Li et al., 2009)

nanos¹⁸/Df(3R)nanos has germline stem cell phenotype, suppressible by bgcn[+]/bgcn^bgcnP.GFP

(Li et al., 2009)

nanos¹⁸/Df(3R)nanos has ovary phenotype, suppressible by bam[+]/bam^BG

(Li et al., 2009)

nanos¹⁸/Df(3R)nanos has germline stem cell phenotype, suppressible by bam[+]/bam^BG

(Li et al., 2009)

nanos¹⁸/Df(3R)nanos has ovary phenotype, suppressible by bam^EP667/bam[+]

(Li et al., 2009)

nanos¹⁸/Df(3R)Delta-FX3 has germline cell | female | larval stage phenotype, suppressible | partially by dpp^UAS.cSa/Scer\GAL4^{VP16.nanos.UTR}

(Gilboa and Lehmann, 2004)

nanos¹⁸/Df(3R)Delta-FX3 has germline cell | female | larval stage phenotype, suppressible | partially by Scer\GAL4^{VP16.nanos.UTR}/tkv^QD.UAS.cUa

(Gilboa and Lehmann, 2004)

nanos¹⁸/Df(3R)Delta-FX3 has germline cyst | ectopic phenotype, suppressible | partially by Scer\GAL4^{VP16.nanos.UTR}/tkv^QD.UAS.cUa

(Gilboa and Lehmann, 2004)

nanos¹⁸/Df(3R)Delta-FX3 has germline cyst | ectopic phenotype, suppressible | partially by dpp^UAS.cSa/Scer\GAL4^{VP16.nanos.UTR}

(Gilboa and Lehmann, 2004)

nanos¹⁸ has egg phenotype, suppressible by Hmag\nanos1^nos.UTR

(Mochizuki et al., 2000)

nanos¹⁸ has phenotype, suppressible by cup¹³

(Verrotti and Wharton, 2000)

nanos¹⁸ has phenotype, suppressible by cup¹⁵

(Verrotti and Wharton, 2000)

nanos¹⁸ has phenotype, suppressible by cup²⁰

(Verrotti and Wharton, 2000)

nanos¹⁸ has phenotype, suppressible by cup²⁴

(Verrotti and Wharton, 2000)

nanos¹⁸ has phenotype, suppressible by cup²⁶

(Verrotti and Wharton, 2000)

nanos¹⁸ has phenotype, suppressible by cup³

(Verrotti and Wharton, 2000)

nanos¹⁸ has phenotype, suppressible by cup¹

(Verrotti and Wharton, 2000)

nanos¹⁸ has egg chamber phenotype, suppressible by cup²¹/cup[+]

(Verrotti and Wharton, 2000)

nanos¹⁸ has egg chamber phenotype, suppressible by cup[+]/cup⁸

(Verrotti and Wharton, 2000)

nanos¹⁸ has ovary phenotype, suppressible by cup²¹/cup[+]

(Verrotti and Wharton, 2000)

nanos¹⁸ has ovary phenotype, suppressible by cup[+]/cup⁸

(Verrotti and Wharton, 2000)

NOT suppressed by

Statement

Reference

nanos¹⁸ has abdominal segment phenotype, non-suppressible by Hmag\nanos1^nos.UTR

(Mochizuki et al., 2000)

Enhancer of

Statement

Reference

nanos¹⁸/nos[+] is an enhancer of female germline stem cell phenotype of twin^DG24102

(Joly et al., 2013)

Suppressor of

Statement

Reference

nanos¹⁸/nos[+] is a suppressor of female germline stem cell | increased number phenotype of Scer\GAL4^{VP16.nanos.UTR}, stwl^EY05697

(Maines et al., 2007)

NOT Suppressor of

Statement

Reference

nanos¹⁸/nos[+] is a non-suppressor of ovary phenotype of cup^unspecified

(Verrotti and Wharton, 2000)

Other

Statement

Reference

cup²¹, nanos¹⁸ has embryonic abdominal segment phenotype

(Verrotti and Wharton, 2000)

cup⁸, nanos¹⁸ has embryonic abdominal segment phenotype

(Verrotti and Wharton, 2000)

cup²¹/cup[+], nanos¹⁸ has embryonic abdominal segment phenotype

(Verrotti and Wharton, 2000)

cup[+]/cup⁸, nanos¹⁸ has embryonic abdominal segment phenotype

(Verrotti and Wharton, 2000)

Additional Comments

Genetic Interactions

Statement

Reference

The dendritic arborization defects observed in class IV dendritic arborizing neurons of nos¹⁸/nos⁵³ third instar larvae are suppresed by combination with a single copy of hid^A206 or by expression of any of the following: Dronc^KK104278, Drice^GD12284 or Dcp-1^KK106134 under the control of Scer\GAL4⁴⁷⁷ in the mutant background.

(Bhogal et al., 2016)

The loss of germline stem cells seen in the ovaries of homozygous twin^DG24102 females is accelerated if the females are also heterozygous for nos¹⁸.

(Joly et al., 2013)

Tumour growth is strongly suppressed in the ovaries of snf¹⁴⁸/snf¹⁴⁸ ; nos¹⁸/Df(3R)Exel6183 females. In young animals, 35% of germaria are agametic, In older animals the fraction of agametic germaria increases to 74%, with a marked reduction in tumour size compared with similar aged snf¹⁴⁸/snf¹⁴⁸ females. The majority of surviving double mutant germ cells have abnormal fusome-like structures and fail to differentiate (indicating that they are more similar to snf than nos mutant germ cells).

(Chau et al., 2012)

Ovaries of nos¹⁸/Df(3R)nos females that are also heterozygous for bam^BG or bam^EP667 produce many egg chambers. Removing one copy of bam increases egg chamber formation at least 10-fold in a nos¹⁸/Df(3R)nos background.

Ovaries of nos¹⁸/Df(3R)nos females that are also heterozygous for bgcn^{bgcnP.T:Avic\GFP} produce many egg chambers. Removing this copy of bgcn increases egg chamber formation in nos¹⁸/Df(3R)nos mutants.

(Li et al., 2009)

The phenotype of extra germline stem cells that is seen in the germarium of females expressing stwl^EY05697 under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} is suppressed if the females also carry nos¹⁸/+.

(Maines et al., 2007)

The precocious differentiation of primordial germ cells which is seen in nos¹⁸/Df(3R)Dl-FX3 larval ovaries is partially suppressed by expression of dpp^Scer\UAS.cSa or tkv^QD.Scer\UAS under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16}.

(Gilboa and Lehmann, 2004)

Third larval instar class I dendritic arborization neurons of third instar larvae which are mutant for both pum¹ and nos¹⁸ (generated using the MARCM technique) do not have any defects in dendrite morphology. Third larval instar class III dendritic arborization neurons which are mutant for both pum¹ and nos¹⁸ (generated using the MARCM technique) show an increase in the number of long dendritic spikes to a similar extent as that seen in the single mutant class III dendritic arborization neurons. Third larval instar class IV dendritic arborization neurons which are mutant for both pum¹ and nos¹⁸ (generated using the MARCM technique) show incomplete innervation of the epidermal territory in about 18% of neurons, an extent similar to that seen in the single mutant class IV dendritic arborization neurons.

(Ye et al., 2004)

The oogenesis defects seen in hemizygous nos¹⁸ females are substantially suppressed by one copy of cup⁸ or cup²¹. Many egg chambers in the rescued ovaries appear normal and mature into oocytes that are fertilised and oviposited (the resulting embryos develop no abdominal segments). The rescued females lay eggs for at least 3 weeks.

(Verrotti and Wharton, 2000)

Xenogenetic Interactions

Statement

Reference

Hmag\nos1^nos.UTR partially rescues the egg laying defects of nos¹⁸ females. Hmag\nos1^nos.UTR fails to rescue the abdominal defects of embryos derived from nos¹⁸ females.

(Mochizuki et al., 2000)

Complementation and Rescue Data

Rescued by

nanos¹⁸/Df(3R)Delta-X43 is rescued by nanos^gnanosb

(Brechbiel and Gavis, 2008)

nanos⁵³/nanos¹⁸ is rescued by nanos^18xTag:MS2

(Brechbiel and Gavis, 2008)

nanos⁵³/nanos¹⁸ is rescued by nanos^{+2.18xTag:MS2}

(Brechbiel and Gavis, 2008)

nanos⁵³/nanos¹⁸ is rescued by nanos^hs.PW

(Wang and Lin, 2004)

nanos¹⁸ is rescued by nanos^ΔF

(Verrotti and Wharton, 2000)

nanos¹⁸ is rescued by nanos^+t5.7

(Verrotti and Wharton, 2000)

nanos¹⁸ is rescued by nanos^ΔG

(Verrotti and Wharton, 2000)

nanos¹⁸ is rescued by nanos^ΔA

(Verrotti and Wharton, 2000)

nanos¹⁸ is rescued by nanos^ΔD

(Verrotti and Wharton, 2000)

nanos¹⁸ is rescued by nanos^ΔB

(Verrotti and Wharton, 2000)

nanos¹⁸ is rescued by nanos^ΔC

(Verrotti and Wharton, 2000)

nanos¹⁸ is rescued by nanos^ΔE

(Verrotti and Wharton, 2000)

nanos¹⁸/Df(3R)Delta-X43 is rescued by nanos^{108.αTub84B.UTR.Tag:HA}