FlyBase Allele Report: Dmel\nanos[53]

General Information

Symbol

Dmel\nanos⁵³

Species

D. melanogaster

Name

FlyBase ID

FBal0013152

Feature type

allele

Associated gene

Dmel\nanos

Associated Insertion(s)

Carried in Construct

Also Known As

nos^RD

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

loss of function allele

(Bhat, 1999)

Mutagen

ethyl methanesulfonate

(Tearle and Nusslein-Volhard, 1987)

Progenitor genotype

Cytology

Description

Amino acid replacement: C354Y. C354 is the is the first cysteine residue in the second CCHC motif. Nucleotide substitution: G1943A.

(Curtis et al., 1997)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3R:19,159,177..19,159,177 [+]

Nucleotide change:

G19159177A

Reported nucleotide change:

G1943A

Amino acid change:

C354Y | nanos-PA; C335Y | nanos-PB

Reported amino acid change:

C354Y

(Curtis et al., 1997)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

abdomen

(Gavis and Lehmann, 1992)

cystoblast & plasma membrane

(Bhat, 1999)

cystocyte & plasma membrane

(Bhat, 1999)

dendrite | third instar larval stage (with nanos¹⁸)

(Bhogal et al., 2016)

egg chamber (with nanos¹⁸)

(Wang and Lin, 2004)

embryonic/larval neuromuscular junction | third instar larval stage (with Df(3R)Exel6183)

(Menon et al., 2009)

female germline stem cell

(Bhat, 1999)

female germline stem cell & plasma membrane

(Bhat, 1999)

female germline stem cell | somatic clone

(Gilboa and Lehmann, 2004)

germarium

(Bhat, 1999)

germline cyst (with nanos¹⁸)

(Wang and Lin, 2004)

germline stem cell (with Df(3R)nanos)

(Li et al., 2009)

larval multidendritic class IV neuron | late third instar larval stage (with nanos¹⁸)

(Brechbiel and Gavis, 2008)

larval multidendritic class IV neuron | third instar larval stage (with nanos¹⁸)

(Bhogal et al., 2016)

mitochondrion

(Bhat, 1999)

NMJ bouton | third instar larval stage (with Df(3R)Exel6183)

(Menon et al., 2009)

ovariole

(Bhat, 1999)

ovariole (with nanos¹⁸)

(Wang and Lin, 2004)

ovary (with Df(3R)nanos)

(Li et al., 2009)

telson

(Gavis and Lehmann, 1992)

testis

(Bhat, 1999)

Detailed Description

Statement

Reference

nos¹⁸/nos⁵³ transheterozygote third instar larvae show dendritic field coverage defects and loss of terminal branches in class IV dendritic arborizing neurons.

(Bhogal et al., 2016)

Females carrying nos¹⁸/Df(3R)nos alleles display ovaries that are largely agametic.

(Li et al., 2009)

nos⁵³/Df(3R)Exel6183 third instar larvae have a significantly higher total number of boutons at the neuromuscular junction (muscle 6/7), compared to controls. Recordings from the NMJ of nos⁵³/Df(3R)Exel6183 larvae show a significant decrease in mEJP (mini) amplitude (but no difference in evoked excitatory junctional potential, eEJP, amplitudes), compared to controls; mEJPs in mutants also have significantly faster decay times compared to controls.

(Menon et al., 2009)

Late third instar nos¹⁸/nos⁵³ larvae show a significant reduction in the density of terminal dendritic branches of the class IV da neurons.

(Brechbiel and Gavis, 2008)

Homozygous germ line stem cell (GSC) clones are rapidly lost from the adult ovary; GSC loss is seen as early as 4 days after clone induction, and by the 6th or 7th day after clone induction, most ovarioles do not contain a mutant GSC.

(Gilboa and Lehmann, 2004)

Ovaries of hemizygous females or nos¹⁸/nos⁵³ transheterozygotes contain ovarioles with either no egg chambers or 1-7 egg chambers of different stages. Some of the ovarioles produce only one egg. Ovaries with no developing germ cells occur. Mutant females show three types of egg laying pattern. TypeI (11% hemizygotes): Egg deposition begins on day 2 and stops by day 5. TypeII (78% hemizygotes): Egg deposition begins on day 2 and stops around day 5, to resume after 2 to 12 days. Type III (11% hemizygotes): No eggs are laid (ovarioles devoid of germ cells). 0.5 day old mutant females have 40% ovarioles indistinguishable from wild type, indicating that ovariole development can initially be normal. Germline defects are age-dependent. Germaria eventually lose stem cells. Plasma membrane degenerates in stem cells and their descendents. The nuclear membrane is unaffected. Germaria from older females contain an abnormally high number of mitochondria. 20% of hemizygous or nos¹⁸/nos⁵³ males show morphologically distinct testis defects: fewer sperm bundles with a large pool of primary spermatocytes filling most of the anterior region of the testis. Number of primary spermatocytes is increased twofold (for hemizygote) compared to wild type. The defective testis generally has fewer coils than wild type.

(Bhat, 1999)

Homozygous embryos show mirror image duplications of posterior abdominal structures and telson structures. Defect in oogenesis so females only produce small numbers of eggs, presence of the P{nos-bcd3'UTR} construct does not rescue this defect.

(Gavis and Lehmann, 1992)

Large amounts of vas protein are expressed in early stages of oogenesis. vas protein localizes to the posterior pole at the same stage of egg development as wild type.

(Lasko and Ashburner, 1990)

nos^L7/nos⁵³ transheterozygous embryos derived from homozygous females have polar granules and pole cells and have deletions of abdominal structures. CycB and vas transcript localization and accumulation is as in wild type.

(Raff et al., 1990)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

nanos⁵³/nanos¹⁸ has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by hid^A206/hid[+]

(Bhogal et al., 2016)

nanos⁵³/nanos¹⁸ has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by Dronc^KK104278/Scer\GAL4⁴⁷⁷

(Bhogal et al., 2016)

nanos⁵³/nanos¹⁸ has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by Drice^GD12284/Scer\GAL4⁴⁷⁷

(Bhogal et al., 2016)

nanos⁵³/nanos¹⁸ has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by Dcp-1^KK106134/Scer\GAL4⁴⁷⁷

(Bhogal et al., 2016)

Phenotype Manifest In

Suppressed by

Statement

Reference

nanos⁵³/nanos¹⁸ has larval multidendritic class IV neuron | third instar larval stage phenotype, suppressible by Dcp-1^KK106134/Scer\GAL4⁴⁷⁷

(Bhogal et al., 2016)

nanos⁵³/nanos¹⁸ has dendrite | third instar larval stage phenotype, suppressible by Dcp-1^KK106134/Scer\GAL4⁴⁷⁷

(Bhogal et al., 2016)

nanos⁵³/nanos¹⁸ has larval multidendritic class IV neuron | third instar larval stage phenotype, suppressible by hid^A206/hid[+]

(Bhogal et al., 2016)

nanos⁵³/nanos¹⁸ has dendrite | third instar larval stage phenotype, suppressible by hid^A206/hid[+]

(Bhogal et al., 2016)

nanos⁵³/nanos¹⁸ has larval multidendritic class IV neuron | third instar larval stage phenotype, suppressible by Dronc^KK104278/Scer\GAL4⁴⁷⁷

(Bhogal et al., 2016)

nanos⁵³/nanos¹⁸ has dendrite | third instar larval stage phenotype, suppressible by Dronc^KK104278/Scer\GAL4⁴⁷⁷

(Bhogal et al., 2016)

nanos⁵³/nanos¹⁸ has larval multidendritic class IV neuron | third instar larval stage phenotype, suppressible by Drice^GD12284/Scer\GAL4⁴⁷⁷

(Bhogal et al., 2016)

nanos⁵³/Df(3R)nanos has ovary phenotype, suppressible by bam[+]/bam^BG

(Li et al., 2009)

nanos⁵³/Df(3R)nanos has germline stem cell phenotype, suppressible by bam[+]/bam^BG

(Li et al., 2009)

nanos⁵³/Df(3R)nanos has ovary phenotype, suppressible by bam^EP667/bam[+]

(Li et al., 2009)

nanos⁵³/Df(3R)nanos has germline stem cell phenotype, suppressible by bam^EP667/bam[+]

(Li et al., 2009)

NOT suppressed by

Statement

Reference

nanos⁵³ has ovary phenotype, non-suppressible by cup^unspecified

(Verrotti and Wharton, 2000)

Additional Comments

Genetic Interactions

Statement

Reference

The dendritic arborization defects observed in class IV dendritic arborizing neurons of nos¹⁸/nos⁵³ third instar larvae are suppresed by combination with a single copy of hid^A206 or by expression of any of the following: Dronc^KK104278, Drice^GD12284 or Dcp-1^KK106134 under the control of Scer\GAL4⁴⁷⁷ in the mutant background.

(Bhogal et al., 2016)

Ovaries of nos⁵³ females that are also heterozygous for bam^BG or bam^EP667 produce many egg chambers. Removing one copy of bam increases egg chamber formation by approximately 10% in a nos⁵³/Df(3R)nos background.

(Li et al., 2009)

The oogenesis defects seen in nos⁵³ females are not modified by cup^unspecified. No modification of the cup^unspecified phenotype is seen in cup^unspecified ; nos¹⁸/+ ovaries.

(Verrotti and Wharton, 2000)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

nanos⁵³/nanos¹⁸ is rescued by nanos^18xTag:MS2

(Brechbiel and Gavis, 2008)

nanos⁵³/nanos¹⁸ is rescued by nanos^{+2.18xTag:MS2}

(Brechbiel and Gavis, 2008)

nanos⁵³/nanos¹⁸ is rescued by nanos^hs.PW

(Wang and Lin, 2004)

Partially rescued by

Df(3R)Exel6183/nanos⁵³ is partially rescued by nanos^{αTub84B.3'UTR.UAS}/Scer\GAL4^Mhc.PU

(Menon et al., 2009)

Not rescued by

nanos⁵³/nanos¹⁸ is not rescued by nanos^{+1.18xTag:MS2}

(Brechbiel and Gavis, 2008)

Comments

Expression of nos^{αTub84B.3'UTR.Scer\UAS} driven by Scer\GAL4^Mhc.PU partially rescues mEJP amplitude and decay time at the NMJ of nos⁵³/Df(3R)Exel6183 third instar larvae.

(Menon et al., 2009)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Lehmann.

Comments

Strong allele of nos. Cytoplasmic transplantation of wild type plasm into the abdominal region restores normal abdominal development: surviving adults are fertile.

(Lehmann and Nusslein-Volhard, 1991)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

nanos⁵³

nanos^RD

(Jankovics et al., 2001)

nos⁵³

(Joly et al., 2013)

nos^RD

(Bhogal et al., 2016, Olesnicky et al., 2012, Harris et al., 2011, Li et al., 2009, Menon et al., 2009, Brechbiel and Gavis, 2008, Verrotti and Wharton, 2000, Arrizabalaga and Lehmann, 1999, Bhat, 1999, Curtis et al., 1997, Murata and Wharton, 1995, Gavis and Lehmann, 1992, Lehmann and Nusslein-Volhard, 1991)

Name Synonyms

Secondary FlyBase IDs

References (21)

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