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General Information
Symbol
Dmel\miraL44
Species
D. melanogaster
Name
FlyBase ID
FBal0082443
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Inserted_sequence:

CAC

Comment:

The G in codon 104 of mira has been replaced with CAC leading to a frameshift and early translation termination after the addition of 33 novel amino acids. The last wild type amino acid is F103.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Nucleotide 544 (G) has been replaced with CAC, which produces a frameshift after amino acid residue 103 (the protein produced contains 33 novel amino acid residues, which are PLRLPVTRIRPPEAEVAPQQRHPLLCIRQPPRP).

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

miraL44 mutants exhibit ectopic gcm expression and an increase in glial number (4-5) accompanied by the lack of the neuronal progeny.

All progeny of NB6-4T adopt a glial cell fate, expressing gcm and repo and moving towards the midline.

Axon tracts of embryos are grossly defective, longitudinal connectives are generally broken or severely reduced in width and commissures are partially or completely fused.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement
Reference
Phenotype Manifest In
Suppressed by
Statement
Reference
Enhancer of
Statement
Reference

miraL44/mira[+] is an enhancer of egg chamber phenotype of cup21

miraL44/mira[+] is an enhancer of egg chamber phenotype of cup15

Other
Additional Comments
Genetic Interactions
Statement
Reference

Over-expression of CycEScer\UAS.cBa under the control of Scer\GAL4sca-109-68 in a miraL44 background is sufficient to confer neuronal fate to the NB6-4t lineage.

The ovarian phenotype of cup15 and cup21 homozygous females is enhanced if they are also heterozygous for miraL44; a greater fraction of egg chambers arrest development between stages 3 and 5 in the double mutant ovaries compared to in the single mutant ovaries.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
Comments
Comments

One of a group of six alleles isolated in the same screen.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
References (13)