FlyBase Allele Report: Dmel\mira[ZZ176]

General Information

Symbol

Dmel\mira^ZZ176

Species

D. melanogaster

Name

FlyBase ID

FBal0082440

Feature type

allele

Associated gene

Dmel\mira

Associated Insertion(s)

Carried in Construct

Also Known As

mir^ZZ176

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

loss of function allele

(Ikeshima-Kataoka et al., 1997)

Mutagen

ethyl methanesulfonate

(Ikeshima-Kataoka et al., 1997)

Progenitor genotype

Cytology

Description

Deletion of nucleotides 1581-1749, which results in a frameshift after amino acid residue 446 (the protein produced contains 14 novel amino acid residues, which are RTWPRRSPRSTTTS).

(Fuerstenberg et al., 1998)

169 bp deletion that causes a frameshift followed immediately by a stop codon. This produces a truncated protein lacking the C-terminal 384 amino acids, including the pros binding domain.

(Ikeshima-Kataoka et al., 1997)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

3R:19,933,333..19,933,335 [-]

Comment:

A 169bp deletion causes a frameshift, followed by a stop codon

(Ikeshima-Kataoka et al., 1997)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of brain cancer

CEA

(Caussinus and Gonzalez, 2005)

model of cancer

CEA

(Janic et al., 2010)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal | recessive

(Ikeshima-Kataoka et al., 1997)

Phenotype Manifest In

embryonic/larval cell body glial cell

(Freeman and Doe, 2001)

larval longitudinal connective

(Ikeshima-Kataoka et al., 1997)

larval neuron

(Freeman and Doe, 2001)

symmetrical commissure

(Ikeshima-Kataoka et al., 1997)

Detailed Description

Statement

Reference

The transplantation of mira^ZZ176 larval neuroblast clones into the abdomen of adult hosts causes tumor growth, with clones growing to 100 times their original size thereby severely damaging and displacing the host's organs. Although genome stability is not grossly affected shortly after transplantation, 40-day-old tumors show karyotype defects such as segmental aneuploidy. Additionally, 15-20% of cells within the tumors have supernumerary centrosomes and these cells tend to be hyperploid.

(Caussinus and Gonzalez, 2005)

Mutant embryos have a variable phenotype in the NGB 6-4T lineage, showing reduced, normal or extra NGB 6-4T-derived glia. Neuronal progeny of the NGB 6-4T lineage are completely absent in 46% of hemisegments but may be normal in other hemisegments.

(Freeman and Doe, 2001)

Axon tracts of embryos are grossly defective, longitudinal connectives are generally broken or severely reduced in width and commissures are partially or completely fused.

(Ikeshima-Kataoka et al., 1997)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

mira^L44

(Ikeshima-Kataoka et al., 1997)

mira^RR127

(Ikeshima-Kataoka et al., 1997)

Not rescued by

mira^ZZ176 is not rescued by Scer\GAL4^unspecified/mira^{5A.UAS.Tag:HA}

(Atwood and Prehoda, 2009)

Comments

mira^{5A.Scer\UAS.T:Ivir\HA1} expression is not able to rescue embryonic lethality of mira^ZZ176 animals.

(Atwood and Prehoda, 2009)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

One of a group of six alleles isolated in the same screen.

(Ikeshima-Kataoka et al., 1997)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

Mir^ZZ176

(Fuerstenberg et al., 1998)

mir^ZZ176

(Freeman and Doe, 2001)

mira^ZZ176

(Wu et al., 2023, Janic et al., 2010, Caussinus and Gonzalez, 2005)

miranda⁸⁸¹⁷⁶

(Ikeshima-Kataoka et al., 1997)

miranda^ZZ176

(Atwood and Prehoda, 2009)

miranda^ZZ

(Fuerstenberg et al., 1998)

Name Synonyms

Secondary FlyBase IDs

References (9)

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