A kel cDNA fragment encoding ORF1 (residues Ser17 to Met688), and tagged after amino acid residue 19 with Tag:MYC, is expressed under the control of an otu promoter (nucleotides 1 to 740 of the genomic sequence in FBrf0050545). The fs(1)K10 3' untranslated region containing transcription termination sequences is fused to the 3' end of the kel cDNA fragment.
No deleterious effects are seen when kelORF1.otu.T:Hsap\MYC is expressed in a wild-type background.