FlyBase Allele Report: Dmel\Nf1[hs.PT]

General Information

Symbol

Dmel\Nf1^hs.PT

Species

D. melanogaster

Name

heat shock construct of The

FlyBase ID

FBal0061706

Feature type

allele

Associated gene

Dmel\Nf1

Associated Insertion(s)

Carried in Construct

P{hsNF1}

Key Links

Transgenic product class

wild_type

(The et al., 1997)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(The et al., 1997)

Mutagen

in vitro construct

(The et al., 1997)

Progenitor genotype

Carried in construct

P{hsNF1}

Cytology

Description

An Hsp70 promoter drives expression of Nf1. The construct includes the shorter Nf1 C-terminus predicted by exon 18b and lacks a 30 amino acid segment predicted by alternatively spliced exon 14.

(Walker et al., 2006)

Hybrid cDNA genomic Nf1 minigene (contains 3 introns) is expressed from an Hsp70 promoter.

(The et al., 1997)

Allele components

Component

Use(s)

Regulatory region(s)

Hsp70

Encoded product / tool

Nf1

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal heat stress response

(Tong et al., 2007)

abnormal locomotor rhythm | heat sensitive

(Tong et al., 2007)

abnormal oxidative stress response | heat sensitive

(Tong et al., 2007)

increased fecundity

(Tong et al., 2007)

long lived | heat sensitive

(Tong et al., 2007)

wild-type

(Yager et al., 2001, Guo et al., 1997, The et al., 1997)

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of Nf1^hs.PT greatly increases the resistance to paraquat-induced oxidative stress, compared to wild-type.

Expression of Nf1^hs.PT in a wild-type background increases aconitase activity by more than 90%.

Upregulation of Nf1^hs.PT expression by maintaining these flies at 25[o]C throughout life results in an increase in mean life span of 49% for male flies and 68% for females and an increase in maximum life span of 38% for males and 52% for females. By contrast, when Nf1^hs.PT flies are maintained at 25[o]C through embryogenesis and 4 days in adulthood but then switched to 18[o]C for the remainder of their adult life, results in the elimination of the extension of maximum life span and a reduction in the extension of mean life span. The mortality rates of these flies do not change compared to wild-type.

Both fertility and fecundity are higher in Nf1^hs.PT-expressing flies compared to wild-type. This is not because the Nf1^hs.PT flies are bigger, as they have similar body lengths to controls, although Nf1^hs.PT females are slightly heavier.

Testing the stress resistance of Nf1^hs.PT-overexpressing flies by assessing up-climbing ability reveals that these flies are highly resistant to heat stress and paraquat-induced oxidative stress, with Nf1^hs.PT females showing a 56% increase in survival time and the males a 51% increase in survival time during paraquat exposure, relative to controls. However Nf1^hs.PT flies are not more resistant to desiccation.

Mitochondria NADH-linked (pyruvate+malate) respiration rates from Nf1^hs.PT overexpressing flies are 25%-38% higher than those of controls, in the presence of ADP, but not in the absence of ADP. Consequently, the respiratory control ratio is significantly higher for these mitochondria than for controls. Nf1^hs.PT-overexpressing mitochondria have a 54% higher ATP production rate when metabolising NADH-linked substrates, compared to controls. In contrast, the respiration rates recorded when using the FADH[[2]]-linked complex II substrate succinate are essentially the same as in controls, with or without ADP.

Complex I activity is 28% higher in Nf1^hs.PT-expressing flies compared to controls.

Mitochondrial H[[2]]O[[2]] production is reduced by 58-59% in Nf1^hs.PT mutants compared to control flies. This reduced mitochondrial ROS is also associated with increased mitochondrial aconitase activity throughout life.

(Tong et al., 2007)

Expression of Nf1^hs.PT in a wild-type background using heat shock has no effect on learning scores.

(Guo et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Nf1^hs.PT rescues Nf1^E2

(Tsai et al., 2012)

Nf1^hs.PT rescues Nf1^P2

(Tong et al., 2007)

Nf1^hs.PT rescues Nf1^E2

(Walker et al., 2006)

Nf1^hs.PT rescues Nf1^P2

(Yager et al., 2001)

Nf1^hs.PT rescues Nf1^P1

(Guo et al., 1997)

Nf1^hs.PT rescues Nf1^P2

(Guo et al., 1997)

Nf1^hs.PT rescues Nf1^P1

(The et al., 1997)

Nf1^hs.PT rescues Nf1^P2

(The et al., 1997)

Partially rescues

Nf1^hs.PT partially rescues Nf1^P1

(Williams et al., 2001)

Nf1^hs.PT partially rescues Nf1^P2

(Williams et al., 2001)

Nf1^hs.PT partially rescues Nf1^P2

(Guo et al., 2000)

Comments

Nf1^hs.PT rescues the neuromuscular junction overgrowth phenotype seen in Nf1^E2/Nf1^E2 larvae. The reduced body size of Nf1^E2/Nf1^E2 pupae is also rescued.

(Tsai et al., 2012)

The presence of Nf1^hs.PT rescues the short life span seen in Nf1^P2 mutants.

The paraquat sensitivity of Nf1^P2 mutant flies is eliminated in Nf1^hs.PT/+; Nf1^P2 mutants.

Mitochondrial aconitase activity is restored by expression of Nf1^hs.PT in a Nf1^P2 mutant background.

Elevated superoxide levels in Nf1^P2 mutant flies are reduced to wild-type levels by the introduction of Nf1^hs.PT.

(Tong et al., 2007)

The pupal size defect seen in Nf1^E2 animals is rescued by expression of Nf1^hs.PT using heat shock.

(Walker et al., 2006)

Addition of Nf1^hs.PT to Nf1^P2 animals fully rescues the perineurial glial thickness phenotype.

(Yager et al., 2001)

Rescues the learning defects of Nf1^P2 flies when expressed using heat shock during adult stages. Expression of Nf1^hs.PT using a single heat shock during larval stages does not rescue the smaller body size of Nf1^P2 flies.

(Guo et al., 2000)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Nf1^hs.PT

Name Synonyms

heat shock construct of The

(The et al., 1997)

Secondary FlyBase IDs

References (8)

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