FlyBase Allele Report: Dmel\SP[UAS.Tag:M(hGGT1)]

General Information

Symbol

Dmel\SP^{UAS.Tag:M(hGGT1)}

Species

D. melanogaster

Name

FlyBase ID

FBal0062250

Feature type

allele

Associated gene

Dmel\SP

Associated Insertion(s)

Carried in Construct

P{UAS-SP.GGT}

Also Known As

UAS-mSP

Key Links

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Nakayama et al., 1997, Aigaki and Nakayama, 1994)

Progenitor genotype

Carried in construct

P{UAS-SP.GGT}

Cytology

Description

UASt regulatory sequences drive expression of mature SP (lacking the signal sequence) that is tagged at the N-terminal end with Tag:M(hGGT1). This results in a membrane-bound protein: the SP portion is expressed extracellularly, but remains attached to the expressing cell (via the membrane located Tag:M(hGGT1) tag).

(Nakayama et al., 1997)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Tagged with

Tag:M(hGGT1)

membrane protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal courtship behavior | female, with Scer\GAL4^dsx.KI

(Rezaval et al., 2012)

abnormal courtship behavior | female, with Scer\GAL4^fru.P1.D

(Yang et al., 2009)

abnormal courtship behavior | female, with Scer\GAL4^ppk.PG

(Yang et al., 2009)

abnormal mating behavior | female, with Scer\GAL4^ChAT.7.4

(Rezaval et al., 2012)

abnormal mating behavior | female, with Scer\GAL4^dsx.KI

(Rezaval et al., 2012)

abnormal mating behavior | female, with Scer\GAL4^fru.P1.D

(Yang et al., 2009)

abnormal mating behavior | female, with Scer\GAL4^ppk.PG

(Yang et al., 2009)

abnormal mating behavior | virgin female limited, with Scer\GAL4^dsx.KI

(Haussmann et al., 2013)

abnormal mating behavior | virgin female limited, with Scer\GAL4^fru.P1.D

(Haussmann et al., 2013)

abnormal mating behavior | virgin female limited, with Scer\GAL4^ppk.PG

(Haussmann et al., 2013)

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of SP^{Scer\UAS.T:Hsap\GGT1} in virgin females using Scer\GAL4^ppk.PG, Scer\GAL4^fru.P1.D or Scer\GAL4^dsx.KI reduces receptivity (readiness to mate) compared with wild-type and increases oviposition similar to mating.

(Haussmann et al., 2013)

Virgin females expressing Acp70A^{Scer\UAS.T:Hsap\GGT1} under the control of Scer\GAL4^dsx.KI show levels of sexual receptivity comparable to mated control females, which are significantly lower than the receptivity of virgin control females. They also show significant rejection behaviours toward courting males, as demonstrated by an increased frequency of ovipositor extrusions, and show an increase in oviposition, similar to the levels seen in mated control females. Compared to control virgin females, the mutant virgin females show a marked decrease in locomotion while being courted and also show reduced levels of activity while the male is not courting.

Wild-type naive males court virgin females expressing Acp70A^{Scer\UAS.T:Hsap\GGT1} under the control of Scer\GAL4^dsx.KI significantly less than control virgin females, but this reduction in courtship index is not seen in experiments with decapitated mutant virgin females.

The reduction in sexual receptivity and increase in oviposition seen in virgin females expressing Acp70A^{Scer\UAS.T:Hsap\GGT1} under the control of Scer\GAL4^dsx.KI compared to control virgin females is partially rescued if the flies also carry Scer\GAL80^ppk.PY.

Virgin females expressing Acp70A^{Scer\UAS.T:Hsap\GGT1} under the control of Scer\GAL4^Cha.7.4 show reduced sexual receptivity compared to control virgin females.

(Rezaval et al., 2012)

Virgin females expressing Acp70A^{Scer\UAS.T:Hsap\GGT1} under the control of Scer\GAL4^fru.P1.D show a strong decrease in sexual receptivity accompanied by rejection behaviours toward courting males (including ovipositor extrusion) and an increase in egg laying to a comparable extent to mated wild-type females.

Virgin females expressing Acp70A^{Scer\UAS.T:Hsap\GGT1} under the control of Scer\GAL4^ppk.PG show a decrease in sexual receptivity accompanied by rejection behaviours toward courting males and an increase in egg laying to a comparable extent to mated wild-type females.

Virgin females expressing Acp70A^{Scer\UAS.T:Hsap\GGT1} under the control of either Scer\GAL4^Ilp7.PY or Scer\GAL4^Or67d-GAL4-1 show normal postmating behaviours.

The reduction in sexual receptivity and increase in oviposition seen in virgin females expressing Acp70A^{Scer\UAS.T:Hsap\GGT1} under the control of Scer\GAL4^fru.P1.D compared to control virgin females is largely rescued if the flies also carry Scer\GAL80^ppk.PY.

(Yang et al., 2009)

Scer\GAL4^C357, Scer\GAL4^55B, Scer\GAL4^C420, Scer\GAL4^C370 and Scer\GAL4^C502b-mediated expression causes only a slight drop in female receptivity whereas ovulation is very low. Scer\GAL4^29BD, Scer\GAL4^156Y, Scer\GAL4^19Y, Scer\GAL4^9Y and Scer\GAL4^7B-mediated expression allows no female receptivity but stimulated very high levels of ovulation.

(Nakayama et al., 1997)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

SP^{UAS.Tag:M(hGGT1)}, Scer\GAL4^fru.P1.D has abnormal mating behavior | virgin female limited phenotype, suppressible by Df(1)JC70/Df(1)Exel6234

(Haussmann et al., 2013)

SP^{UAS.Tag:M(hGGT1)}, Scer\GAL4^ppk.PG has abnormal mating behavior | virgin female limited phenotype, suppressible by Df(1)JC70/Df(1)Exel6234

(Haussmann et al., 2013)

SP^{UAS.Tag:M(hGGT1)}, Scer\GAL4^dsx.KI has abnormal mating behavior | virgin female limited phenotype, suppressible by Df(1)JC70/Df(1)Exel6234

(Haussmann et al., 2013)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

In virgin Df(1)JC70/Df(1)Exel6234 mutant females, expression of SP^{Scer\UAS.T:Hsap\GGT1} under the control of Scer\GAL4^dsx.KI results in increased oviposition compared with wild-type but does not affect receptivity.

Virgin Df(1)JC70/Df(1)Exel6234 mutant females expressing SP^{Scer\UAS.T:Hsap\GGT1} under the control of Scer\GAL4^fru.P1.D behave like wild-type virgin females and do not reduce receptivity nor increase oviposition.

Virgin Df(1)JC70/Df(1)Exel6234 mutant females expressing SP^{Scer\UAS.T:Hsap\GGT1} under the control of Scer\GAL4^ppk.PG behave like wild-type virgin females and do not reduce receptivity nor increase oviposition.

(Haussmann et al., 2013)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Acp70A^{Scer\UAS.T:Hsap\GGT1}

Acp70A^UAS.T:GGT1

SP^{Scer\UAS.T:Hsap\GGT1}

SP^{UAS.Tag:M(hGGT1)}

Name Synonyms

Secondary FlyBase IDs

References (10)

Report Sections

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