FlyBase Allele Report: Dmel\inaD[T1]

General Information

Symbol

Dmel\inaD^T1

Species

D. melanogaster

Name

FlyBase ID

FBal0062794

Feature type

allele

Associated gene

Dmel\inaD

Associated Insertion(s)

Carried in Construct

Also Known As

inaD¹

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Tsunoda et al., 1997)

Progenitor genotype

Cytology

Description

Amino acid replacement: ?270term.

(Tsunoda et al., 1997)

Mutation is the second PDZ domain.

(Tsunoda et al., 1997)

Nucleotide substitution: ?811?.

(Tsunoda et al., 1997)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2R:22,857,286..22,857,286 [-]

Nucleotide change:

C22857286T

Reported nucleotide change:

?811?

Amino acid change:

Q271term | inaD-PA; Q283term | inaD-PB

Reported amino acid change:

?270term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Tsunoda et al., 1997)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of sensorineural hearing loss

CEA

(Senthilan et al., 2012)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

eye photoreceptor cell

(Meyer et al., 2006)

eye photoreceptor cell, with inaD^C645S.ninaE

(Mishra et al., 2007)

photoreceptor

(Scott and Zuker, 1998)

photoreceptor | adult stage, with inaD^ninaE.T669A

(Liu et al., 2011)

Detailed Description

Statement

Reference

Compound action potentials can be evoked by sound in the antennal nerve of mutant flies, but the sound particle velocities required to elicit the response is increased compared to wild type. The displacement response of the antenna over a range of sound particle velocities is linearised, indicating loss of mechanical amplification.

(Senthilan et al., 2012)

The photoreceptors of newly eclosed flies expressing inaD^ninaE.T669A in a inaD^T1 mutant background show a slower response to brief (1ms) flashes containing ~75 effectively absorbed photons. Peak amplitude is similar to wild type but the time to peak is delayed and the decay time course is slower. Following 1s light steps of increasing intensity wild type responses consist of an initial peak that rapidly declines to minimum (the "notch"); this "notch" is more pronounced in inaD^T1 inaD^ninaE.T669A mutant flies. Although the intensity dependence of both the peak and plateau in mutants are indistinguishable from wild-type, the "notch" develops at 2-3 fold lower intensities than in controls.

(Liu et al., 2011)

The light-triggered translocation of trpl^{ninaE.T:Avic\GFP-EGFP} is abolished in inaD^T1 flies.

(Meyer et al., 2008)

Inhibition of trpl^{ninaE.T:Avic\GFP-EGFP} internalisation is observed in inaD^T1 mutant photoreceptors.

(Meyer et al., 2006)

Mutants have single photon responses that are grossly disorganised, with large defects in amplitude, latency and deactivation. Quantum bumps have amplitudes less than one fifth of those of controls, latencies that are roughly six times greater than wild type and responses that fail to terminate normally, with single photons producing several microbumps. Double mutants with of Cam^unspecified with inaD^T1 show a noisy, continuous response to a single photon of light, and do not produce individual bumps.

(Scott and Zuker, 1998)

inaD^T1 flies have an abnormal electroretinogram (ERG) phenotype, with severe defects in response to light. Loss of responsiveness to light increases with age.

(Tsunoda et al., 1997)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressor of

Statement

Reference

inaD^T1 is a suppressor of rhabdomere phenotype of rdgA¹

(Georgiev et al., 2005)

inaD^T1/inaD^su1 is a suppressor of rhabdomere phenotype of rdgA¹

(Georgiev et al., 2005)

inaD^su100/inaD^T1 is a suppressor of rhabdomere phenotype of rdgA¹

(Georgiev et al., 2005)

Additional Comments

Genetic Interactions

Statement

Reference

The retinal degeneration phenotype of rdgA¹ flies is suppressed by inaD^T1, inaD^T1/inaD^su1 and inaD^T1/inaD^su100.

(Georgiev et al., 2005)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

inaD^ttd10

(Meyer et al., 2008)

Rescued by

inaD^T1 is rescued by inaD^ninaE.PM

(Mishra et al., 2007)

Partially rescued by

inaD^T1 is partially rescued by inaD^GMR.GFP

(Sanxaridis and Tsunoda, 2010)

inaD^T1 is partially rescued by inaD^C645S.ninaE

(Mishra et al., 2007)

Comments

inaD^{GMR.T:Avic\GFP} rescues the electroretinogram response of inaD^T1 flies to near wild type.

(Sanxaridis and Tsunoda, 2010)

Light-raised inaD^ttd10 in trans with inaD^T1 shows partial translocation of trpl^{ninaE.T:Avic\GFP-EGFP} from the rhabdomere to the cell body. It should be noted that the trpl^{ninaE.T:Avic\GFP-EGFP} translocation defect in homozygous inaD^ttd10 mutants is more severe than in trans with inaD^T1. inaD^ttd10/inaD^T1 flies exhibit almost normal internalisation in some photoreceptor cells of each ommatidium whereas the internalisation defect is observed in all photoreceptor cells in homozygous inaD^ttd10 mutants.

(Meyer et al., 2008)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

InaD¹

(Georgiev et al., 2005, Montell, 1998)

inaD¹

(Chen and Montell, 2020, Voolstra et al., 2017, Senthilan et al., 2012, Sanxaridis and Tsunoda, 2010, Venkatachalam et al., 2010, Meyer et al., 2008, Peng et al., 2008, Mishra et al., 2007, Sanxaridis et al., 2007, Meyer et al., 2006, Popescu et al., 2006, Chorna-Ornan et al., 2005, Baehner et al., 2002, Tsunoda et al., 2001, Wes et al., 1999, Scott and Zuker, 1998, Tsunoda et al., 1997)

inaD^T1

(Liu et al., 2011)

Name Synonyms

Secondary FlyBase IDs

References (20)

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