Included in an approximately 4.6-kb EcoRI-XhoI fragment that is just slightly shorter on its 3' end than the 4.8-kb EcoRI fragment carried in Adh+t4.8.
Adh expression has correct tissue specificity but Adh protein level of adult flies is reduced by two fold and levels in third instar larval by 10-fold. Position of restriction fragment on reference sequence inferred by FlyBase curator.