>Our Ref: rd2836.hf
>
>Hi Jim,
>
>I am curating
>
>*x FBrf0076568 == Hodgetts et al., 1994, Genome 37(4): 526-534.
>
>Figure 2 shows vector pARP-11, made by yourself.
>
>In order to capture the details of this paper I need to know a little more
>about pARP-11, such as does the Adh bit correspond to any of these
>Adh alleles?
Now we're really going back into ancient history (the map I have is dated
10/82). The Adh bit in pARP-11 is actually none of these. It is an
approximately 4.6-kb EcoRI/XhoI fragment that is just slightly shorter on
its 3' end than the 4.8-kb EcoRI/EcoRI fragment carried in Adh+t4.8. It's
the same AdhF allele, though.
>and is the ry+ the well used ry+t7.2 (as used in Carnegie 20) or
>another version?
Naturally, I had to do things my own way. I preferred to use a somewhat
larger ry+ piece, an 8.2-kb SalI fragment.
Good luck with your curating, Rachel. Let me know if you have other questions.
Best wishes,
Jim
>
Subject: Re: Helping FlyBase
Hi Rachel,
>I'm 99.9% sure that what you describe as an 8.2-kb SalI fragment must be
>the Rubin and Spradling one represented in FlyBase by the allele:
>*A ry+t8.1
>Could you confirm for me that this is the same one?
Yes, you're right - no question about it.
Best wishes,
Jim