FlyBase Allele Report: Dmel\dbe[k05428]

General Information

Symbol

Dmel\dbe^k05428

Species

D. melanogaster

Name

FlyBase ID

FBal0064487

Feature type

allele

Associated gene

Dmel\dbe

Associated Insertion(s)

P{lacW}dbe^k05428

Carried in Construct

Also Known As

l(2)k05428

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(BDGP Project Members, 1994-1999)

Progenitor genotype

Associated Insertion(s)

P{lacW}dbe^k05428

Cytology

Description

P{lacW} insertion in the 5'UTR, duplicating a target site between positions -49 and -41 (numbered from the first nucleotide of the predicted protein).

(Chan et al., 2001)

Allele components

Component

Use(s)

Inserted element

P{lacW}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2L:869,816..869,816 [+]

(Chan et al., 2001, Spradling et al., 1999, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

cell lethal | recessive | somatic clone

(Chan et al., 2001)

lethal | recessive

(Spradling et al., 1999, BDGP Project Members, 1994-1999)

lethal - all die before end of larval stage | recessive

(Chan et al., 2001)

some die during larval stage | recessive

(Chan et al., 2001)

Phenotype Manifest In

Detailed Description

Statement

Reference

Homozygous larvae appear to be arrested at the first instar stage. They fail to increase in size or and die 2-3 days after hatching without any morphological defects. Homozygous clones in the eye are not recovered, although twin post dbe⁺ clones are found, suggesting that dbe is required for cell viability during eye development.

(Chan et al., 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

dbe^k05447

(BDGP Project Members, 1994-1999)

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

12169

y¹ w^67c23; P{lacW}dbe^k05428/CyO

Kyoto

111429

y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{5xglBS-lacZ.38-1}TPN1; P{lacW}dbe^k05428 P{neoFRT}40A/CyO y⁺

Notes on Origin

Discoverer

I. Kiss.

(BDGP Project Members, 1994-1999)

Comments

The lethality of dbe^k05428 can be reverted by excision of the P{lacW} element.

(Chan et al., 2001)

Complements: l(2)01855⁰¹⁸⁵⁵. Complements: dock⁰⁴⁷²³.

(BDGP Project Members, 1994-1999)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

dbe^P

(Chan et al., 2001)

dbe^k05428

l(2)k05428

(Chan et al., 2001, Spradling et al., 1999)

l(2)k05428^k05428

(BDGP Project Members, 1994-1999)

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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