FlyBase Allele Report: Dmel\Mgtor[k03905]

General Information

Symbol

Dmel\Mgtor^k03905

Species

D. melanogaster

Name

FlyBase ID

FBal0064547

Feature type

allele

Associated gene

Dmel\Mgtor

Associated Insertion(s)

P{lacW}Mgtor^k03905

Carried in Construct

Also Known As

Mtor^k03905

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

Mutagen

P-element activity

Nature of the Allele

Allele class

loss of function allele

(Qi et al., 2004)

Mutagen

P-element activity

(BDGP Project Members, 1994-1999)

Progenitor genotype

Associated Insertion(s)

P{lacW}Mgtor^k03905

Cytology

Description

P{lacW} is inserted at the +1 position of the Mtor cDNA. The insertion causes duplication of the +1 residue and an 8bp upstream genomic sequence.

(Qi et al., 2004)

Allele components

Component

Use(s)

Inserted element

P{lacW}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2R:11,852,136..11,852,601 [+]

(Qi et al., 2004, Spradling et al., 1999, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Mtor^k03905 mutant MARCM clones in central brain neuroblasts show reactivation defects as high proportion of the cells retain cellular process and vast majority are not mitotically active at 96h after larval hatching.

(Li et al., 2017)

In adult testes, Mtor^k03905-homozygous germline stem cell clones are fewer in number than control germline stem cell clones at day 1 after clone induction, becoming negligible or inexistent at days 2 or 7 after clone induction, respectively; at day 1 after clone induction, mosaic testes bearing Mtor^k03905 mutant clones in an otherwise Mtor^k03905 heterozugous background display increased apoptosis as compared to controls, which is limited to outside of the germline stem cell region.

Somatic cyst stem cells are much fewer in number in Mtor^k03905-homozygous clones within mosaic testes, being negligible at days 1 and 2 post clone induction and inexistent at day 4 post clone induction, as compared to control clones within control testes; no cell death is detected in the somatic cyst stem cell lineage of Mtor^k03905-homozygous clones.

(Liu et al., 2015)

Among 300 embryos, only one homozygous Mtor^k03905 first instar larvae emerged.

(Qi et al., 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Mgtor^k03905 has decreased occurrence of cell division | somatic clone phenotype, suppressible | partially by pros[+]/pros¹⁷

(Li et al., 2017)

Mgtor^k03905 has decreased cell number | germline clone | adult stage phenotype, suppressible | partially by Scer\GAL4^{VP16.nanos.UTR}/mad2^UAS.cLa

(Liu et al., 2015)

Mgtor^k03905 has decreased cell number | germline clone | adult stage phenotype, suppressible | partially by Scer\GAL4^{VP16.nanos.UTR}/Mps1^UAS.cLa

(Liu et al., 2015)

Mgtor^k03905 has decreased cell number | germline clone | adult stage phenotype, suppressible | partially by Scer\GAL4^{VP16.nanos.UTR}/Apc2^UAS.cUa

(Liu et al., 2015)

Phenotype Manifest In

Suppressed by

Statement

Reference

Mgtor^k03905 has neuroblast | somatic clone | third instar larval stage phenotype, suppressible | partially by pros[+]/pros¹⁷

(Li et al., 2017)

Mgtor^k03905 has male germline stem cell | adult stage phenotype, suppressible | partially by Scer\GAL4^{VP16.nanos.UTR}/mad2^UAS.cLa

(Liu et al., 2015)

Mgtor^k03905 has male germline stem cell | adult stage phenotype, suppressible | partially by Scer\GAL4^{VP16.nanos.UTR}/Mps1^UAS.cLa

(Liu et al., 2015)

Mgtor^k03905 has male germline stem cell | adult stage phenotype, suppressible | partially by Scer\GAL4^{VP16.nanos.UTR}/Apc2^UAS.cUa

(Liu et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

The reactivation defects observed in central neuroblast Mtor^k03905 MARCM mutant clones are partially rescued by pros¹⁷ heterozygosity.

(Li et al., 2017)

Expressing either mad2^Scer\UAS.cLa or Mps1^Scer\UAS.cLa under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} suppresses the germline stem cell loss present in homozygous Mtor^k03905 germline stem cell clones in adult testes, 2 days post clone induction.

Expression of Apc2^Scer\UAS.cUa under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} partially rescues the decreased number of germline stem cells in homozygous Mtor^k03905 within mosaic testes, as compared to control clones in control testes.

(Liu et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Mgtor^k03905 is rescued by Scer\GAL4^{VP16.nanos.UTR}/Mgtor^UAS.cLa

(Liu et al., 2015)

Comments

Co-expression of Mtor^Scer\UAS.cLa under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} rescues the the germline stem cell loss present in homozygous Mtor^k03905 germline stem cell clones in adult testes, as compared to control clones.

(Liu et al., 2015)

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

10537

y¹ w^67c23; P{lacW}Mgtor^k03905/CyO

Kyoto

111163

y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{5xglBS-lacZ.38-1}TPN1; P{neoFRT}42D P{lacW}Mgtor^k03905 /CyO y⁺

Notes on Origin