FlyBase Allele Report: Dmel\Cse1[k03902]

General Information

Symbol

Dmel\Cse1^k03902

Species

D. melanogaster

Name

FlyBase ID

FBal0064548

Feature type

allele

Associated gene

Dmel\Cse1

Associated Insertion(s)

P{lacW}Cse1^k03902

Carried in Construct

Also Known As

l(2)k03902

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - genetic evidence

Mutagen

P-element activity

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Tekotte et al., 2002)

Mutagen

P-element activity

(Buszczak et al., 2002, Tekotte et al., 2002, Beaton, 2000.1.31, Spradling et al., 1999, FlyBase, 1996-, BDGP Project Members, 1994-1999)

Progenitor genotype

Associated Insertion(s)

P{lacW}Cse1^k03902

Cytology

Description

The P{lacW} insertion is 71bp upstream of the ATG start of Cas.

(Tekotte et al., 2002)

The P{lacW}Cas^k03902 insertion maps within both Cas and mdy (nested genes on opposite strands).

(FlyBase, 1996-)

Allele components

Component

Use(s)

Inserted element

P{lacW}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2L:16,825,128..16,825,128 [+]

(Buszczak et al., 2002, Tekotte et al., 2002, Beaton, 2000.1.31, Spradling et al., 1999, FlyBase, 1996-, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates autosomal dominant cerebellar ataxia

modeled by Hsap\ATXN8OS^CTG112.UAS

(Mutsuddi et al., 2004)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal (with Cse1^EP2592)

(Buszczak et al., 2002)

lethal | recessive

(Buszczak et al., 2002, Beaton, 2000.1.31, Spradling et al., 1999, BDGP Project Members, 1994-1999)

lethal | somatic clone

(Tekotte et al., 2002)

Phenotype Manifest In

Detailed Description

Statement

Reference

Homozygous somatic clones are not recovered.

(Tekotte et al., 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Cas[+]/Cse1^k03902 is an enhancer of lethal phenotype of Kap-α1^UAS.cMa, Scer\GAL4^e22c

(Ratan et al., 2008)

Suppressor of

Statement

Reference

Cas[+]/Cse1^k03902 is a suppressor of abnormal neuroanatomy phenotype of Hsap\ATXN8OS^CTG112.UAS, Scer\GAL4^GMR.PF

(Mutsuddi et al., 2004)

Phenotype Manifest In

Enhancer of

Statement

Reference

Cas[+]/Cse1^k03902 is an enhancer of abdominal tergite phenotype of Kap-α1^UAS.cMa, Scer\GAL4^e22c

(Ratan et al., 2008)

Additional Comments

Genetic Interactions

Statement

Reference

Surviving escapers expressing Kap-α1^Scer\UAS.cMa under the control of Scer\GAL4^e22c in a Cas^k03902/+ background have tergites that appear thinner than normal and retain only the posterior pigmented band, resulting in an apparent expansion of the intertergal region.

(Ratan et al., 2008)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Cse1^k03902 is rescued by Cse1^+tTa

(Tekotte et al., 2002)

Comments

The lethality caused by the P{lacW}Cas^k03902 insertion is due to its effect on Cas and not mdy; the lethality is rescued by Cas^+tTa, which contains the entire Cas gene but lacks the mdy transcription start site and sequences encoding the N-terminal half of the mdy protein.

(Tekotte et al., 2002)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

10536

y¹ w^67c23; P{lacW}Cse1^k03902 mdy^k03902/CyO

Notes on Origin

Discoverer

I. Kiss.

(BDGP Project Members, 1994-1999)

Comments

After six generations of outcrossing the lethality continued to segragate with the P{lacW} insertion.

(Beaton, 2000.1.31)

Precise excision of the P{lacW} element reverts the recessive lethal phenotype. Phenotypic analysis provides an allelic series: Cas^k03902/Df(2L)H20 > Cas^EMS25 > Cas^ts28/Df(2L)H20 > Cas^EMS39.

(Tekotte et al., 2002)

Complements: glu^k08819. Complements: Mhc^k10423. Complements: l(2)k13905^k13905. Complements: l(2)k15102^k15102.

(BDGP Project Members, 1994-1999)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

Cas^k03902

Cse1^k03902

Dcas^l(2)k03902

(Ratan et al., 2008)

l(2)k03902

(Mutsuddi et al., 2004, Tekotte et al., 2002, Spradling et al., 1999)

l(2)k03902^k03902

(BDGP Project Members, 1994-1999)

Name Synonyms

Secondary FlyBase IDs

References (8)

Report Sections

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