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General Information
Symbol
Dmel\shotk03405
Species
D. melanogaster
Name
FlyBase ID
FBal0064571
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
shotkakP2, l(2)k03405, kakP2
Key Links
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

An insertion of a transposable element into the intron between the second and third transcriptional start sites of shot.

An insertion of a transposable element into the intron between the second and third transcriptional start sites of shot. The P-element is inserted at genomic position 9,807,300 (accession number FJ869115).

P{lacW} insertion in an intron 1917bp before the first exon common to mRNAs encoding shot isoforms A and B.

Insertion of a P{lacW} element into the intron at the 5' end of the gene that separates the alternate starts from the shared exons.

Insertion components
P{lacW}shotk03405
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

shotk03405 mutant embryos display no muscle detachment phenotypes.

The dendrites of motorneurons are strongly reduced and motorneuron axons stall in shotk03405 mutant embryos.

shotk03405/Df(2R)MK1 primary neuronal cultures show a significantly reduced axonal length compared to controls. They also show an increase of neurons with unbundled microtubules - these microtubules display irregular trajectories and frequent loops in axons and growth cones.

shot3/Df(2R)MK1 primary neuronal cultures have normal numbers of filopodia.

Germ band retraction and head involution fail in approximately 15% of homozygous embryos. Breaks in the ventral epidermis of the middle abdominal segments are seen in fully germ band extended embryos.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
Comments
Comments

Lethality is reverted by excision of the P{lacW} element.

The site of the P{lacW} insertion in shotk03010 is identical to the site of the P{lacW} insertion in shotk03405.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (7)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (10)