FlyBase Allele Report: Dmel\Src64B[Δ540.hs.2sev]

General Information

Symbol

Dmel\Src64B^{Δ540.hs.2sev}

Species

D. melanogaster

Name

FlyBase ID

FBal0065001

Feature type

allele

Associated gene

Dmel\Src64B

Associated Insertion(s)

Carried in Construct

P{S10-Δ540}

Key Links

Transgenic product class

stop_gained

(Kussick et al., 1993)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

stop_gained

(Kussick et al., 1993)

Mutagen

in vitro construct

(Kussick et al., 1993)

Progenitor genotype

Carried in construct

P{S10-Δ540}

Cytology

Description

Construct: Amino acid replacement: S540. Two copies of the sev enhancer linked to an Hsp70 promoter drive expression of a C-terminally truncated form of Src64B@ (contains the mutation described above) which lacks the putative negative regulatory residue Tyr547.

(Kussick et al., 1993)

Allele components

Component

Use(s)

Encoded product / tool

Src64B

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal | heat sensitive

(Kussick et al., 1993)

visible

(Kussick et al., 1993)

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of Src64B^{Δ540.hs.2sev} under the control of heat shock in early embryos results in dramatic defects in cuticular structures, most notably deletion of the ventral denticle bands. The filzkorper are slightly enlarged.

(Li et al., 2000)

Overexpression of Src64B^{Δ540.hs.2sev} by heat shock at 37^oC for 1 hour every other day during development is lethal. Over 95% of embryos carrying two copies of Src64B^{Δ540.hs.2sev} show abnormalities when Src64B^{Δ540.hs.2sev} is expressed using a 30 minute heat shock at 37^oC between 5 and 8 hours of development. These include failure to undergo germ band retraction and grossly abnormal nervous system morphology, including asymmetries and extreme thinning of the longitudinal connectives. Expression of Src64B^{Δ540.hs.2sev} in adults causes roughening of the eye. The regular array of the ommatidia is grossly distorted. 92% of the ommatidia contain an excess of photoreceptor cells with small rhabdomeres.

(Kussick et al., 1993)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Src64B^{Δ540.hs.2sev} has lethal | heat sensitive phenotype, suppressible by Ras85D^e2F

(Kussick et al., 1993)

Src64B^{Δ540.hs.2sev} has lethal | heat sensitive phenotype, suppressible by Df(3R)by10

(Kussick et al., 1993)

Src64B^{Δ540.hs.2sev} has visible phenotype, suppressible by sina³

(Kussick et al., 1993)

Src64B^{Δ540.hs.2sev} has visible phenotype, suppressible by Ras85D^e1B

(Kussick et al., 1993)

Phenotype Manifest In

Suppressed by

Statement

Reference

Src64B^{Δ540.hs.2sev} has eye phenotype, suppressible by Ras85D^e2F

(Kussick et al., 1993)

Src64B^{Δ540.hs.2sev} has eye phenotype, suppressible by sina³

(Kussick et al., 1993)

Src64B^{Δ540.hs.2sev} has eye phenotype, suppressible by Ras85D^e1B

(Kussick et al., 1993)

Additional Comments

Genetic Interactions

Statement

Reference

The conditional lethal phenotype of animals expressing Src64B^{Δ540.hs.2sev} is suppressed by Ras85D^e2F or Df(3R)by10. 80-90% of Ras85D^e2F/+ flies carrying one copy of Src64B^{Δ540.hs.2sev} show a reduction in eye roughness relative to control flies carrying one copy of Src64B^{Δ540.hs.2sev} in a Ras85D⁺ background; most of the extra R7-like cells are eliminated and much of the normal eye architecture is restored. The rough eye phenotype is also partially suppressed by Ras85D^e1B or sina³.

(Kussick et al., 1993)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Src64B^{Δ540.hs.2sev}

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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