Expression of wgPE2.hs in a wild-type background using a single heat shock at 4 hours of development results in an excess of naked cuticle. Expression of wgPE2.hs in a wild-type background using a single heat shock at 6 hours of development subtly alters the denticle pattern, producing a partial loss or rearrangement of denticles in the second or third row of the denticle belt, but does not induce naked cuticle. Expression of wgPE2.hs by three consecutive heat shocks in wgl-17 embryos restores denticle diversity, but there is no secretion of naked cuticle. Expression of wgPE2.hs using a single heat shock at 6 hours of development in wgl-17 embryos produces reorientation of the denticles toward the ventral midline, but no specification of naked cuticle.