FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\tgoDN.UAS
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General Information
Symbol
Dmel\tgoDN.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0082183
Feature type
allele
Associated gene
Dmel\tgo
Associated Insertion(s)
Carried in Construct
P{UAS-tgo.DN}
Key Links
Transgenic product class
dominant_negative_variant
(Ohshiro and Saigo, 1997)
Mutagen
Nature of the Allele
Transgenic product class
dominant_negative_variant
(Ohshiro and Saigo, 1997)
Mutagen
in vitro construct
(Ohshiro and Saigo, 1997)
Progenitor genotype
Carried in construct
P{UAS-tgo.DN}
Cytology
Description

A dominant negative form of tgo, in which a 13 amino acid basic region (SRENHCEIERRRR) is replaced by the sequence GIL is expressed under the control of four copies of the UAS regulatory sequence and a heat shock promoter.

(Ohshiro and Saigo, 1997)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
tgo
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of tgoDN.Scer\UAS in all glial cells, using Scer\GAL4repo does not result in any impairment of glial migration.

      Expression of tgoDN.Scer\UAS in all tracheal cells using Scer\GAL4btl.PS does not lead to a glial migration phenotype.

      Expression of tgoDN.Scer\UAS under the control of Scer\GAL4loco.1.3 causes a phenocopy of the glial migration phenotype of tgo mutant embryos in the absence of a CNS defect.

      (Schmidt et al., 2011)

      In brains of larvae that express tgoDN.Scer\UAS in developing lamina neurons, driven by Scer\GAL4NP6099, all of the R axon bundles are packed tightly in the posterior domain of the lamina region and almost all of the developing lamina neurons are located outside of the R axon bundles.

      (Umetsu et al., 2006)

      Causes stalling of tracheal migration when expressed using Scer\GAL4btl.PS.

      (Ohshiro and Saigo, 1997)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressed by
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      In larval brains that coexpress tgoDN.Scer\UAS and simScer\UAS.cCa, both under the control of Scer\GAL4NP6099, both the arrangement of lamina neurons and the spacing of R axon bundles are almost indistinguishable from those of control brains.

      (Umetsu et al., 2006)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      tgoDN.Scer\UAS
      tgoDN.UAS
      Name Synonyms
      Secondary FlyBase IDs
        References (3)