FlyBase Allele Report: Dmel\pnt[unspecified]

General Information

Symbol

Dmel\pnt^unspecified

Species

D. melanogaster

Name

FlyBase ID

FBal0082324

Feature type

allele

Associated gene

Dmel\pnt

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Progenitor genotype

Cytology

Description

FlyBase curator comment: this entry is used to capture phenotypic information when the particular allele (or allele combination) used by the author could not be determined but the context of the experiment suggests that the phenotype being described is some kind of loss of function.

(FlyBase Curators, 2025-)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased cell number | embryonic stage | somatic clone

(Alvarez et al., 2003)

lethal | recessive

(Therrien et al., 2000)

lethal - all die during embryonic stage | recessive

(Tearle and Nusslein-Volhard, 1987)

some die during embryonic stage | recessive

(Tearle and Nusslein-Volhard, 1987)

Phenotype Manifest In

cephalopharyngeal skeleton

(Tearle and Nusslein-Volhard, 1987)

dorsal vessel wall cell | ectopic | somatic clone

(Alvarez et al., 2003)

embryonic/larval dorsal branch & terminal tracheal cell | somatic clone

(Ghabrial and Krasnow, 2006)

larval anterior commissure

(Stollewerk and Klämbt, 1997)

larval longitudinal connective

(Hummel et al., 1999)

midline glial cell

(Hummel et al., 1999, Hummel et al., 1999)

motor neuron

(Hummel et al., 1999)

presumptive embryonic/larval central nervous system

(Hummel et al., 1999, Hummel et al., 1999, Tearle and Nusslein-Volhard, 1987)

presumptive embryonic/larval peripheral nervous system

(Hummel et al., 1999)

sensory neuron

(Hummel et al., 1999)

symmetrical commissure

(Hummel et al., 1999, Hummel et al., 1999, Stollewerk and Klämbt, 1997)

ventral denticle belt

(Tearle and Nusslein-Volhard, 1987)

Detailed Description

Statement

Reference

Mosaic dorsal tracheal branches lack a terminal cell in 26% of cases. In 80% of the cases where the terminal cell is missing, a homozygous mutant cell is located in the stalk position nearest the tip.

(Ghabrial and Krasnow, 2006)

When homozygous pnt cardioblast clones are made in the embryo, a small proportion of clones exhibit more cardioblasts than expected, suggesting a slight increase in the proliferative capability of cardioblast precursors.

(Alvarez et al., 2003)

Mutant embryos show a clear fusion of commissures and frequent disruption of longitudinal connectives. Midline glial cells are either reduce in number or fail to properly migrate in between anterior and posterior commissures. General embryonic morphology is relatively normal, though the PNS shows a slight reduction in sensory neurons and defects in the trajectories of motoneurons.

(Hummel et al., 1999)

Fas2-positive nerve bundles are seen to cross the midline in mutant embryos, in contrast to wild-type.

(Hummel et al., 1999)

The axon bundles of the anterior commissures are not organised into three sub-regions in homozygous embryos, in contrast to wild-type. One or two discrete axon bundles can be seen within the commissure. The commissural axons leave the longitudinal tracts at apparently random, irregular positions along the dorso-ventral axis of the connective.

(Stollewerk and Klämbt, 1997)

pnt^unspecified mutant embryos have narrow denticle bands. The central nervous system is broad and less dense than wild-type. The head skeleton has fused ventral arms.

(Tearle and Nusslein-Volhard, 1987)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

pnt^unspecified/pnt[+] is an enhancer of lethal phenotype of Raf¹²

(Therrien et al., 2000)

pnt^unspecified/pnt[+] is an enhancer of visible phenotype of ksr::tor^{ΔNksr.hs.sev}

(Therrien et al., 2000)

Suppressor of

Statement

Reference

pnt^unspecified/pnt[+] is a suppressor of visible phenotype of Ras85D^V12.sev

(Therrien et al., 2000)

Phenotype Manifest In

Enhanced by

Statement

Reference

pnt^unspecified has symmetrical commissure phenotype, enhanceable by foi^unspecified

(Hummel et al., 1999)

pnt^unspecified has symmetrical commissure phenotype, enhanceable by schmalspur^unspecified

(Hummel et al., 1999)

Enhancer of

Statement

Reference

pnt^unspecified/pnt[+] is an enhancer of larval longitudinal connective phenotype of spen^AH393

(Chen and Rebay, 2000)

pnt^unspecified/pnt[+] is an enhancer of central nervous system phenotype of spen^AH393

(Chen and Rebay, 2000)

pnt^unspecified/pnt[+] is an enhancer of eye phenotype of ksr::tor^{ΔNksr.hs.sev}

(Therrien et al., 2000)

pnt^unspecified is an enhancer of symmetrical commissure phenotype of foi^unspecified

(Hummel et al., 1999)

pnt^unspecified is an enhancer of symmetrical commissure phenotype of schmalspur^unspecified

(Hummel et al., 1999)

Suppressor of

Statement

Reference

pnt^unspecified/pnt[+] is a suppressor of eye phenotype of Ras85D^V12.sev

(Therrien et al., 2000)

Additional Comments

Genetic Interactions

Statement

Reference

foi^unspecified pnt^unspecified or sur^unspecified pnt^unspecified double mutant embryos show a slightly enhanced fused commissure phenotype compared to the phenotypes of the single mutants. The number of midline glial cells is reduced in the double mutant embryos. Commissures fail to develop in most neuromeres of Hem^unspecified pnt^unspecified double mutant embryos.

(Hummel et al., 1999)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

pnt^unspecified

Name Synonyms

Secondary FlyBase IDs

References (9)

Report Sections

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