FlyBase Allele Report: Dmel\puc[S023803]

General Information

Symbol

Dmel\puc^S023803

Species

D. melanogaster

Name

FlyBase ID

FBal0082882

Feature type

allele

Associated gene

Dmel\puc

Associated Insertion(s)

P{lacW}puc^S023803

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Delta2-3 transposase

(Deak et al., 1997)

P-element activity

(Szeged Stock Center, 1998-, Salzberg et al., 1997)

Progenitor genotype

Associated Insertion(s)

P{lacW}puc^S023803

Cytology

Description

P{lacW} insertion in the intron between exons 3 and 4 of puc.

(Prokopenko et al., 2000)

Allele components

Component

Use(s)

Inserted element

P{lacW}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3R:8,115,264..8,115,264 [-]

(Prokopenko et al., 2000, Szeged Stock Center, 1998-, Deak et al., 1997, Salzberg et al., 1997)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

AF174657

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal | recessive

(Szeged Stock Center, 1998-, Deak et al., 1997)

lethal - all die during embryonic stage | recessive

(Salzberg et al., 1997)

some die during embryonic stage | recessive

(Salzberg et al., 1997)

Phenotype Manifest In

embryonic/larval dorsal vessel | posterior

(Salzberg et al., 1997)

presumptive embryonic/larval peripheral nervous system

(Salzberg et al., 1997)

Detailed Description

Statement

Reference

Early lethal.

(Deak et al., 1997)

Embryos exhibit defects in heart morphogenesis, the posterior region of the dorsal vessel remains open. Axonal bundles in the PNS of homozygotes appear defasciculated and thicker than those of normal embryos.

(Salzberg et al., 1997)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Complements

puc¹

(Prokopenko et al., 2000)

Fails to complement

puc^A251.1F3

(Prokopenko et al., 2000)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Δ2-3 induced reversion demonstrates the insertion is responsible for the lethal phenotype.

(Salzberg et al., 1997)

External Crossreferences and Linkouts ( 1 )

Crossreferences

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

AF174657

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

0238/03

(Deak et al., 1997)

238/3

(Salzberg et al., 1997)

hrt^S023803

hty^S023803

(Szeged Stock Center, 1998-)

l(3)S023803

(Prokopenko et al., 2000)

puc^S023803

Name Synonyms

Secondary FlyBase IDs

FBal0100362

References (4)

Report Sections

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