FlyBase Allele Report: Dmel\Syx1A[hs.PW]

General Information

Symbol

Dmel\Syx1A^hs.PW

Species

D. melanogaster

Name

heat shock construct of Wu

FlyBase ID

FBal0083729

Feature type

allele

Associated gene

Dmel\Syx1A

Associated Insertion(s)

Carried in Construct

P{hs-syx1A.W}

Key Links

Transgenic product class

cDNA

(Wu et al., 1998)

wild_type

(Wu et al., 1998)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Wu et al., 1998)

wild_type

(Wu et al., 1998)

Mutagen

in vitro construct

(Wu et al., 1998)

Progenitor genotype

Carried in construct

P{hs-syx1A.W}

Cytology

Description

Syx1A cDNA is expressed under the control of an Hsp70 promoter.

(Wu et al., 1998)

Allele components

Component

Use(s)

Regulatory region(s)

Hsp70

Encoded product / tool

Syx1A

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neurophysiology | larval stage | heat sensitive

(Nie et al., 1999, Wu et al., 1998)

viable

(Nie et al., 1999)

Phenotype Manifest In

neuromuscular junction | larval stage

(Nie et al., 1999)

Detailed Description

Statement

Reference

Flies carrying Syx1A^hs.PW are viable and have normal wings and eyes at 25^oC. The mean evoked excitatory junction potential (EJP) amplitude at the neuromuscular junction is reduced by about 34% compared to wild type in larvae expressing Syx1A^hs.PW at 25^oC.

(Nie et al., 1999)

Overexpression of Syx1A^hs.PW using heat shock causes a 67% decrease in the amplitude of the excitatory junctional potential (EJP) in the muscle at the larval neuromuscular junction compared to non-heat shocked larvae. The frequency of miniature EJPs is increased by 67% in heat shocked larvae expressing Syx1A^hs.PW compared to non-heat shocked larvae. Overexpression of both Rop^hs.PS and Syx1A^hs.PW using heat shock partially suppresses the physiological defects seen in the muscle at the larval neuromuscular junction when either is overexpressed alone.

(Wu et al., 1998)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Syx1A^hs.PW is a suppressor | partially of visible phenotype of Csp^2.UAS, Scer\GAL4^elav-C155

(Nie et al., 1999)

Phenotype Manifest In

Suppressed by

Statement

Reference

Syx1A^hs.PW has neuromuscular junction | larval stage phenotype, suppressible by Scer\GAL4^elav-C155/Csp^2.UAS

(Nie et al., 1999)

Syx1A^hs.PW has neuromuscular junction | larval stage phenotype, suppressible by Scer\GAL4^elav-C155/Csp^3.UAS

(Nie et al., 1999)

Suppressor of

Statement

Reference

Syx1A^hs.PW is a suppressor | partially of wing phenotype of Csp^2.UAS, Scer\GAL4^elav-C155

(Nie et al., 1999)

Syx1A^hs.PW is a suppressor | partially of eye phenotype of Csp^2.UAS, Scer\GAL4^elav-C155

(Nie et al., 1999)

Additional Comments

Genetic Interactions

Statement

Reference

The crumpled wing and rough eye phenotype of adult escapers carrying Csp^2.Scer\UAS expressed under the control of Scer\GAL4^elav-C155 is partially suppressed by Syx1A^hs.PW at 25^oC and completely suppressed by daily heat shocks during late larval and pupal development in these flies (which increase expression of Syx1A^hs.PW). The EJP amplitude is normal if larvae expressing Syx1A^hs.PW are also expressing Csp^2.Scer\UAS or Csp^3.Scer\UAS under the control of Scer\GAL4^elav-C155.

(Nie et al., 1999)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Syx1A^hs.PW

syx1A^hs.PW

(Nie et al., 1999)

Name Synonyms

heat shock construct of Wu

(Wu et al., 1998)

Secondary FlyBase IDs

References (2)

Report Sections

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