FlyBase Allele Report: Dmel\Csp[3.UAS]

General Information

Symbol

Dmel\Csp^3.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0104344

Feature type

allele

Associated gene

Dmel\Csp

Associated Insertion(s)

Carried in Construct

P{UAS-Csp.3}

Key Links

Transgenic product class

cDNA

(Nie et al., 1999)

wild_type

(Nie et al., 1999)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Nie et al., 1999)

wild_type

(Nie et al., 1999)

Mutagen

in vitro construct

(Nie et al., 1999)

Progenitor genotype

Carried in construct

P{UAS-Csp.3}

Cytology

Description

UASt regulatory sequences drive expression of the Csp type 3 cDNA.

(Nie et al., 1999)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Csp

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

eye, with Scer\GAL4^elav-C155

(Nie et al., 1999)

interommatidial bristle, with Scer\GAL4^elav-C155

(Nie et al., 1999)

ommatidium, with Scer\GAL4^elav-C155

(Nie et al., 1999)

wing, with Scer\GAL4^elav-C155

(Nie et al., 1999)

Detailed Description

Statement

Reference

Expression of Csp^3.Scer\UAS under the control of Scer\GAL4^elav-C155 in a wild-type background has no significant effect on evoked excitatory junction potential amplitude at the neuromuscular junction in larvae. Features of spontaneous release are also normal in these larvae. Expression of Csp^3.Scer\UAS under the control of Scer\GAL4^elav-C155 or Scer\GAL4^hs.PB results in temperature-sensitive lethality, with the animals dying during the first larval instar stage at 29^oC. The lethality caused by expression of Csp^3.Scer\UAS under the control of Scer\GAL4^hs.PB is more severe in male than in female flies. Adult escapers carrying Csp^3.Scer\UAS expressed under the control of Scer\GAL4^elav-C155 have a short life span, dying within the 10 to 21 days at 25^oC. The flies are extremely sluggish, show severely uncoordinated movements, and slip frequently. Body size is severely reduced and wing inflation is impaired, so that the wings appear severely crumpled. The regular ommatidial pattern is disrupted so that the eye appears rough. The eye is slightly elliptical in shape and some bristles are lost. Fusion of several ommatidial units is occasionally seen. The crumpled wing phenotype is suppressed if the flies are also homozygous for Csp^R1. The rough eye phenotype is partially suppressed if the flies are also carrying Csp^R1.

(Nie et al., 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressor of

Statement

Reference

Scer\GAL4^elav-C155/Csp^3.UAS is a suppressor of neuromuscular junction | larval stage phenotype of Syx1A^hs.PW

(Nie et al., 1999)

Additional Comments

Genetic Interactions

Statement

Reference

The mean evoked excitatory junction potential (EJP) amplitude at the neuromuscular junction is reduced by about 34% compared to wild type in larvae expressing Syx1A^hs.PW at 25^oC. The EJP amplitude is normal if the larvae are also expressing Csp^3.Scer\UAS under the control of Scer\GAL4^elav-C155.

(Nie et al., 1999)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescues

Scer\GAL4^elav-C155/Csp^3.UAS partially rescues Csp^R1

(Nie et al., 1999)

Comments

Expression of Csp^3.Scer\UAS under the control of Scer\GAL4^elav-C155 has no effect on spontaneous miniature excitatory junction potentials at the neuromuscular junction of Csp^R1 third instar larvae, but does rescue the evoked excitatory junction potential at the neuromuscular junction.

(Nie et al., 1999)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Csp^3.Scer\UAS

Csp^3.UAS

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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