FlyBase Allele Report: Dmel\Scr[hs.PP]

General Information

Symbol

Dmel\Scr^hs.PP

Species

D. melanogaster

Name

heat shock construct of Percival-Smith

FlyBase ID

FBal0086755

Feature type

allele

Associated gene

Dmel\Scr

Associated Insertion(s)

Carried in Construct

P{hs-Scr.P}

Key Links

Transgenic product class

missense_variant

(Percival-Smith et al., 1997)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Percival-Smith et al., 1997)

Mutagen

in vitro construct

(Percival-Smith et al., 1997)

Progenitor genotype

Carried in construct

P{hs-Scr.P}

Cytology

Description

Full length Scr gene (same as wild type except for two amino acid replacements: N226R, K227S) is expressed from an Hsp70 promoter.

(Percival-Smith et al., 1997)

Allele components

Component

Use(s)

Regulatory region(s)

Hsp70

Encoded product / tool

Scr

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

visible

(Percival-Smith et al., 1997)

Phenotype Manifest In

arista

(Percival-Smith et al., 1997)

larval mesothoracic segment

(Percival-Smith and Bondy, 1999, Percival-Smith et al., 1997)

larval metathoracic segment

(Percival-Smith and Bondy, 1999, Percival-Smith et al., 1997)

salivary gland imaginal ring

(Percival-Smith et al., 1997)

T1 beard | ectopic

(Percival-Smith and Bondy, 1999, Percival-Smith et al., 1997)

tarsal segment | ectopic

(Percival-Smith et al., 1997)

Detailed Description

Statement

Reference

Expression of Scr^hs.PP using a 50 minute heat shock at 5.5 hours of development results in 72% of first instar larvae having an ectopic T1 beard in thoracic segment T2 and/or T3.

(Percival-Smith and Bondy, 1999)

Heat induced expression results in transformation of aristae into tarsi, maxillary palps are not transformed to proboscis. Ectopic expression results in the formation of ectopic T1 beards in T2 and T3. Ectopic coexpression with pb^hs.PP or pb^Q50K.hs suppresses T1 beard formation in all three thoracic segments. Ectopic coexpression of Scr^hs.PP and pb^hs.PP at 3 hours AEL does not affect the ectopic salivary gland phenotype caused by ectopic Scr expression alone.

(Percival-Smith et al., 1997)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressed by

Statement

Reference

Scr^hs.PP has T1 beard | ectopic phenotype, suppressible by Mmus\Hoxa2^hs.PP

(Percival-Smith and Bondy, 1999)

Scr^hs.PP has larval mesothoracic segment phenotype, suppressible by Mmus\Hoxa2^hs.PP

(Percival-Smith and Bondy, 1999)

Scr^hs.PP has larval metathoracic segment phenotype, suppressible by Mmus\Hoxa2^hs.PP

(Percival-Smith and Bondy, 1999)

Scr^hs.PP has T1 beard | ectopic phenotype, suppressible by pb^hs.PP

(Percival-Smith and Bondy, 1999)

Scr^hs.PP has larval mesothoracic segment phenotype, suppressible by pb^hs.PP

(Percival-Smith and Bondy, 1999)

Scr^hs.PP has larval metathoracic segment phenotype, suppressible by pb^hs.PP

(Percival-Smith and Bondy, 1999)

Additional Comments

Genetic Interactions

Statement

Reference

The Scr^hs.PP ectopic T1 beard phenotype is suppressed by coexpression of pb^hs.PP (4% of larvae have ectopic T1 beards).

(Percival-Smith and Bondy, 1999)

Xenogenetic Interactions

Statement

Reference

The Scr^hs.PP ectopic T1 beard phenotype is suppressed by coexpression of Mmus\Hoxa2^hs.PP (41% of larvae have ectopic T1 beards).

(Percival-Smith and Bondy, 1999)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Scr^hs.PP

Name Synonyms

heat shock construct of Percival-Smith

(Percival-Smith et al., 1997)

Secondary FlyBase IDs

References (2)

Report Sections

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