FlyBase Allele Report: Dmel\sax[ΔI.UAS]

General Information

Symbol

Dmel\sax^ΔI.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0092534

Feature type

allele

Associated gene

Dmel\sax

Associated Insertion(s)

Carried in Construct

P{UAS-sax.ΔI}

Also Known As

UAS-saxΔI

Key Links

Transgenic product class

dominant_negative_variant

(Haerry et al., 1998)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

dominant_negative_variant

(Haerry et al., 1998)

Mutagen

in vitro construct

(Haerry et al., 1998, Nguyen et al., 1998)

Progenitor genotype

Carried in construct

P{UAS-sax.ΔI}

Cytology

Description

A dominant negative form of sax in which the kinase domain is deleted (stop codon after amino acid residue A261) is expressed under the control of UAS regulatory sequences.

(Haerry et al., 1998)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

sax

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

visible, with Scer\GAL4^A9

(Haerry et al., 1998, Nguyen et al., 1998)

visible, with Scer\GAL4^Bx-MS1096

(Evans et al., 2009)

visible, with Scer\GAL4^ptc-559.1

(Haerry et al., 1998)

Phenotype Manifest In

anterior crossvein, with Scer\GAL4^ptc-559.1

(Haerry et al., 1998)

dorsal row of triple row of wing sensilla | proximal, with Scer\GAL4^A9

(Haerry et al., 1998)

embryonic/larval dorsal tracheal branch, with Scer\GAL4^btl.PS

(Steneberg et al., 1999)

embryonic/larval dorsal tracheal branch, with Scer\GAL4^esg-p127

(Steneberg et al., 1999)

medial row of triple row of wing sensilla | proximal, with Scer\GAL4^A9

(Haerry et al., 1998)

posterior crossvein, with Scer\GAL4^A9

(Haerry et al., 1998, Nguyen et al., 1998)

ventral row of triple row of wing sensilla | proximal, with Scer\GAL4^A9

(Haerry et al., 1998)

wing, with Scer\GAL4^A9

(Nguyen et al., 1998)

wing blade, with Scer\GAL4^Bx-MS1096

(Evans et al., 2009)

wing disc, with Scer\GAL4^A9

(Haerry et al., 1998)

wing vein, with Scer\GAL4^Bx-MS1096

(Evans et al., 2009)

wing vein L2, with Scer\GAL4^A9

(Haerry et al., 1998)

wing vein L5, with Scer\GAL4^A9

(Haerry et al., 1998, Nguyen et al., 1998)

Detailed Description

Statement

Reference

Expression of sax^ΔI.Scer\UAS driven by Scer\GAL4^Bx-MS1096 induces extensive defects in longitudinal wing vein formation and wing patterning.

(Evans et al., 2009)

When expression is driven by Scer\GAL4^p127 in the fusion cells disrupts fusion in 19% of the dorsal branches. Suppression of expression of hdc suggests a failure of fusion cell differentiation. When expression is driven by Scer\GAL4^btl.PS a similar, though milder, phenotype results.

(Steneberg et al., 1999)

Animals expressing sax^ΔI.Scer\UAS under the control of Scer\GAL4^A9 have small wing discs. In the adult wing, vein L5 and the posterior crossvein are lost. Vein L2 is shifted more proximally and proximal triple row bristles are replaced by more proximal costa bristles. The distance between veins L3 and L4 is normal, however, the overall shape of the wing is more "strap-like" than normal suggesting loss of peripheral tissue. Flies expressing sax^ΔI.Scer\UAS under the control of Scer\GAL4^ptc-559.1 lack the anterior crossvein at the anterior/posterior boundary of the wing.

(Haerry et al., 1998)

Flies expressing two copies of sax^ΔI.Scer\UAS under the control of Scer\GAL4^A9 have narrower wings than wild-type which lack vein L5 and the posterior crossvein. Flies expressing one copy of sax^ΔI.Scer\UAS under the control of Scer\GAL4^A9 have no wing patterning defects.

(Nguyen et al., 1998)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Scer\GAL4^A9/sax^ΔI.UAS is a suppressor of visible phenotype of Scer\GAL4^A9, gbb^UAS.cKa

(Haerry et al., 1998)

sax^ΔI.UAS is a suppressor of visible phenotype of Scer\GAL4^A9, scw^UAS.cNa

(Nguyen et al., 1998)

NOT Suppressor of

Statement

Reference

Scer\GAL4^A9/sax^ΔI.UAS is a non-suppressor of visible phenotype of Scer\GAL4^A9, dpp^UAS.cUa

(Haerry et al., 1998, Nguyen et al., 1998)

Phenotype Manifest In

Suppressor of

Statement

Reference

Scer\GAL4^A9/sax^ΔI.UAS is a suppressor of wing phenotype of Scer\GAL4^A9, gbb^UAS.cKa

(Haerry et al., 1998)

sax^ΔI.UAS is a suppressor of wing phenotype of Scer\GAL4^A9, scw^UAS.cNa

(Nguyen et al., 1998)

sax^ΔI.UAS is a suppressor of wing vein phenotype of Scer\GAL4^A9, scw^UAS.cNa

(Nguyen et al., 1998)

NOT Suppressor of

Statement

Reference

Scer\GAL4^A9/sax^ΔI.UAS is a non-suppressor of wing phenotype of Scer\GAL4^A9, dpp^UAS.cUa

(Haerry et al., 1998, Nguyen et al., 1998)

Additional Comments

Genetic Interactions

Statement

Reference

The wing phenotype caused by expression of gbb^Scer\UAS.cKa under the control of Scer\GAL4^A9 is almost completely suppressed if the flies are also coexpressing sax^ΔI.Scer\UAS. The wing phenotype caused by expression of dpp^Scer\UAS.cHa under the control of Scer\GAL4^A9 is not suppressed if the flies are also coexpressing sax^ΔI.Scer\UAS.

(Haerry et al., 1998)

Co-expression of one copy of sax^ΔI.Scer\UAS strongly suppresses the wing phenotype caused by scw^Scer\UAS.cNa expressed under the control of Scer\GAL4^A9. Co-expression of one copy of sax^ΔI.Scer\UAS has no effect on the wing phenotype caused by dpp^Scer\UAS.cHa expressed under the control of Scer\GAL4^A9.

(Nguyen et al., 1998)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

604929

P{UAS-sax.ΔI}3, w^*

604928

y¹ w^*; P{UAS-sax.ΔI}11A2; P{UAS-sax.ΔI}11A3

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

sax^ΔI.Scer\UAS

sax^ΔI.UAS

saxΔI

(Haerry et al., 1998)

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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