FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\asl3
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General Information
Symbol
Dmel\asl3
Species
D. melanogaster
Name
FlyBase ID
FBal0093160
Feature type
allele
Associated gene
Dmel\asl
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
ethyl methanesulfonate
(Bonaccorsi et al., 1998)
Progenitor genotype
Cytology
Description

71bp deletion from nucleotides 2381-2452. This results in a shift in the reading frame and introduces a premature stop codon at position 795.

(Varmark et al., 2007)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In

      anaphase & condensed nuclear chromosome (with asl2)

      (Giansanti et al., 2001)

      centrosome (with asl2)

      (Giansanti et al., 2001)

      ciliary rootlet (with asl2)

      (Chen et al., 2015)

      larval brain & ganglion mother cell (with asl2)

      (Bonaccorsi et al., 2000)

      larval brain & ganglion mother cell & aster (with asl2)

      (Bonaccorsi et al., 2000)

      larval brain & ganglion mother cell & centrosome (with asl2)

      (Bonaccorsi et al., 2000)

      larval brain & neuroblast (with asl2)

      (Bonaccorsi et al., 2000)

      larval brain & neuroblast & aster (with asl2)

      (Bonaccorsi et al., 2000)

      larval brain & neuroblast & centrosome (with asl2)

      (Bonaccorsi et al., 2000)

      microtubule (with asl2)

      (Giansanti et al., 2001)

      microtubule basal body & spermatocyte (with Df(3R)ED5177)

      (Blachon et al., 2008)

      mitosis & nuclear chromosome (with asl2)

      (Giansanti et al., 2001)

      neuroblast & nucleus (with asl2)

      (Giansanti et al., 2001)

      spermatocyte & aster (with asl1)

      (Bonaccorsi et al., 1998)

      spermatocyte & aster (with asl2)

      (Bonaccorsi et al., 1998)
      Detailed Description
      Statement
      Reference

      Hemizygous asl3 neuroblasts show no sign of centrosome-organized asters and divide at changing angles at each cell cycle.

      (Januschke and Gonzalez, 2010)

      asl3/Df(3R)ED5177 spermatocytes completely lack basal bodies.

      (Blachon et al., 2008)

      The mitotic index and frequencies of metaphase and anaphase figures in asl2/asl3 larval brains is comparable to wild type. 4-5% of anaphases have lagging chromosomes. A small fraction of metaphases appear to be polyploid; 5-6% hyperploid and 2.6-2.8% tetraploid metaphases are seen. asl2/asl3 larval neuroblasts lack functional centrosomes and are completely devoid of astral microtubules. However, they form central spindles that are indistinguishable from wild type. The central spindle is tightly associated with the nascent ganglion mother cell nucleus, which is in turn closely apposed to the polar cortex, as occurs in wild-type cells. However, wild-type and mutant telophases differ in the positioning of the neuroblast (NB) nucleus; in wild-type, the NB nucleus lies very close to the central spindle but is separated from the polar cortex by a large astral array of microtubules, however the situation is reversed in mutant telophases; the NB nucleus is usually disconnected from the central spindle and located much closer to the polar cortex than in wild type.

      (Giansanti et al., 2001)

      Lethality acts at the larval/pupal boundary. Prophase neuroblasts are completely devoid of centrosomes and asters, however a bipolar spindle does form and generates a normal central spindle in anaphase. Chromosome segregation and cytokinesis appear to be normal. Mitosis in GMCs reveals an absence of centrosomes and prophase asters.

      (Bonaccorsi et al., 2000)

      asl2/asl3 larvae die at the larval/pupal boundary. Homozygous asl3 animals have an earlier lethal phase. asl3/asl1 animals are viable. asl2/asl3 spermatocytes are completely devoid of asters.

      (Bonaccorsi et al., 1998)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Fails to complement

      asl2

      (Bonaccorsi et al., 2000)

      asl1

      (Bonaccorsi et al., 1998)
      Comments

      Fails to complement asl1 for male and female sterility and aberrant spermatid phenotype.

      (Bonaccorsi et al., 1998)
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      References (9)