Deletion of the RS2 domain (amino acids 193 to 264).
tra2ΔRS.N.Tag:FLAG fails to rescue tra2B
Generally fails to rescue somatic sexual differentiation of XX tra2B flies (female abdominal pigmentation, number of sixth sternite bristles, female genitalia and number of sex combs are not rescued), although some lines show slight rescue, having a slight rotation of otherwise male genitalia, indicating intersexuality. Female and male fertility is not rescued.