FlyBase Allele Report: Dmel\tra2[B]

General Information

Symbol

Dmel\tra2^B

Species

D. melanogaster

Name

FlyBase ID

FBal0017022

Feature type

allele

Associated gene

Dmel\tra2

Associated Insertion(s)

Carried in Construct

Also Known As

tra-2^B, tra-2B

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - genetic evidence

loss of function allele

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Mattox and Baker, 1991)

loss of function allele

(Dauwalder et al., 1996, Hazelrigg and Tu, 1994)

Mutagen

ethyl methanesulfonate

Progenitor genotype

Cytology

Description

Nonsense codon within the RRM that truncates translation of all three tra2 isoforms deleting the RNP1 region of the RRM as well as the entire essential RS2 domain.

(Mattox et al., 1996)

Amino acid replacement: ??term.

(Mattox and Baker, 1991)

Nucleotide substitution: C?T.

(Mattox and Baker, 1991)

Mutation is within exon 5, which creates an in-frame premature stop codon that is expected to cause truncation of the polypeptides encoded by each of the major tra2 transcripts at a position within the putative RNA-binding domain and 155 amino acids upstream from their shared carboxyl terminus.

(Mattox and Baker, 1991)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2R:14,603,232..14,603,232 [-]

Nucleotide change:

C14603232T

Amino acid change:

Comment:

altered sequence presented in Figure 4 of FBrf0054067; introduces stop codon in the polypeptides encoded by each of the major tra2 transcripts, 155 amino acids upstream from their shared carboxyl terminus

(Mattox and Baker, 1991)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal sex-determination (with Df(2R)trix)

(Andrews and Oliver, 2002)

abnormal sex-determination | female (with Df(2R)trix)

(Sarno et al., 2011, Pane et al., 2005)

abnormal sex-determination | recessive

(Dauwalder and Mattox, 1998, Chandler et al., 1997, Mattox et al., 1996)

female sterile | recessive

(Chandler et al., 1997)

male sterile | recessive

(Chandler et al., 1997)

Phenotype Manifest In

Detailed Description

Statement

Reference

Both tra2^B/tra2^ts1 transheterozygotes shifted from 18[o]C into 29[o]C upon reaching adulthood and tra2^B/Df(2R)trix transheterozygotes do not show significant mitotic index differences in female and male adult posterior midguts, as compared to controls.

(Hudry et al., 2016)

Adult XX tra2^B/Df(2R)trix pseudomales have a sex comb on the first leg, which contains a similar number of bristles to that of their XY brothers.

(Sarno et al., 2011)

XX ; tra2^B/Df(2R)trix animals develop as pseudomales.

(Pane et al., 2005)

About 10% of 2X tra2^B/Df(2R)trix males have a well developed germline with advanced primary spermatocytes, spermatids or sperm. The remaining animals have gonads containing mostly debris.

(Andrews and Oliver, 2002)

Heterozygous tra2^B/+ adults have normal sexual differentiation.

(Du et al., 1998)

XX homozygotes are sterile, develop into somatic males, and develop sex combs. XY homozygotes are sterile.

(Chandler et al., 1997)

Individuals are transformed into males. Heat induced expression of P{hs-htra-2α} causes female sexual differentiation.

(Dauwalder et al., 1996)

Homozygous chromosomal females appear male, with well developed sex combs.

(Mattox et al., 1996)

No more than 26% of XX tra2 mutant pseudomales produce gonads with the most severe group 1 pseudotestes phenotype. The remaining pseudomales have a less severe phenotype, with viable germ cells. otu¹⁰ and Df(1)otu-PΔ1 cause an increase in severity of this phenotype: over 70% of the resulting gonads were of group 1.

(Nagoshi et al., 1995)

Females exhibit segment spanning muscles in the fifth abdominal segment indicative of a transformation to the male specific phenotype. Somatic development of males is unaffected.

(Taylor, 1992)

tra2^B/tra2^B identical to tra2^B/tra2 or to tra2^B/Df(2R)L4 (Baker and Ridge, 1980).

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

tra2^B/Df(2R)trix has abnormal sex-determination | female phenotype, suppressible | partially by Scer\GAL4^rn-GAL4/Aobl\tra2^UAS.cSa

(Sarno et al., 2011)

tra2^B has abnormal sex-determination | recessive phenotype, suppressible by tra::tra2^C.Tag:FLAG

(Dauwalder and Mattox, 1998)

tra2^B has abnormal sex-determination | recessive phenotype, suppressible by tra2::U2af50^C.Tag:FLAG

(Dauwalder and Mattox, 1998)

tra2^B has abnormal sex-determination | recessive phenotype, suppressible by tra2::U2af50^N.Tag:FLAG

(Dauwalder and Mattox, 1998)

tra2^B has abnormal sex-determination | recessive phenotype, suppressible by tra2::U2af50^C.lacZ

(Dauwalder and Mattox, 1998)

tra2^B has abnormal sex-determination | recessive phenotype, suppressible by B52::tra2^C.lacZ

(Dauwalder and Mattox, 1998)

tra2^B has male sterile phenotype, suppressible by tra2::U2af50^N.Tag:FLAG

(Dauwalder and Mattox, 1998)

tra2^B has abnormal sex-determination | recessive phenotype, suppressible by tra2::Hsap\SRSF2^C.Tag:FLAG

(Dauwalder and Mattox, 1998)

tra2^B has abnormal sex-determination | recessive phenotype, suppressible by tra2::Hsap\SRSF2^N.Tag:FLAG

(Dauwalder and Mattox, 1998)

NOT suppressed by

Statement

Reference

tra2^B/Df(2R)trix has abnormal sex-determination | female phenotype, non-suppressible by Ccap\tra^hs.PP

(Pane et al., 2005)

Other

Statement

Reference

tra2::U2af50^N.Tag:FLAG, tra2^B has female fertile phenotype

(Dauwalder and Mattox, 1998)

tra2::U2af50^C.lacZ, tra2^B has female fertile phenotype

(Dauwalder and Mattox, 1998)

B52::tra2^C.lacZ, tra2^B has female fertile phenotype

(Dauwalder and Mattox, 1998)

Doa⁷, tra2^B has abnormal sex-determination | female phenotype

(Du et al., 1998)

Doa⁷/Doa^unspecified, tra2^B has abnormal sex-determination | dominant | female phenotype

(Du et al., 1998)

Doa^unspecified, tra2^B has abnormal sex-determination | female phenotype

(Du et al., 1998)

Dvir\tra2^tCa, tra2^B has male fertile phenotype

(Chandler et al., 1997)

fru¹, tra2^B has abnormal courtship behavior phenotype

(Ryner et al., 1996)

Phenotype Manifest In

Enhanced by

Statement

Reference

tra2^B has phenotype, enhanceable by B52^ED

(Peng and Mount, 1995)

Suppressed by

Statement

Reference

tra2^B/Df(2R)trix has sex comb | ectopic | female phenotype, suppressible | partially by Scer\GAL4^rn-GAL4/Aobl\tra2^UAS.cSa

(Sarno et al., 2011)

NOT suppressed by

Statement

Reference

tra2^B has phenotype, non-suppressible by snf¹

(Andrews and Oliver, 2002)

tra2^B has phenotype, non-suppressible by otu^unspecified

(Andrews and Oliver, 2002)

tra2^B has phenotype, non-suppressible by B52::tra2^A.N.Tag:FLAG

(Dauwalder and Mattox, 1998)

tra2^B has phenotype, non-suppressible by B52::tra2^B.N.Tag:FLAG

(Dauwalder and Mattox, 1998)

tra2^B has phenotype, non-suppressible by B52::tra2^C.Tag:FLAG

(Dauwalder and Mattox, 1998)

tra2^B has phenotype, non-suppressible by B52::tra2^N.Tag:FLAG

(Dauwalder and Mattox, 1998)

Enhancer of

Statement

Reference

tra2^B/tra2[+] is an enhancer of male genitalia phenotype of Aobl\tra^fl.UAS, Scer\GAL4^arm.PS

(Ruiz and Sanchez, 2010)

tra2^B/Df(2R)trix is an enhancer of female genitalia phenotype of Aobl\tra^fl.UAS, Scer\GAL4^da.G32

(Ruiz and Sanchez, 2010)

Suppressor of

Statement

Reference

tra2^B/Df(2R)trix is a suppressor of sex comb phenotype of Aobl\tra^fl.UAS, Scer\GAL4^da.G32

(Ruiz and Sanchez, 2010)

tra2^B/Df(2R)trix is a suppressor of sex comb tooth phenotype of Aobl\tra^fl.UAS, Scer\GAL4^da.G32

(Ruiz and Sanchez, 2010)

tra2^B/Df(2R)trix is a suppressor of epandrium phenotype of Aobl\tra^fl.UAS, Scer\GAL4^da.G32

(Ruiz and Sanchez, 2010)

tra2^B/Df(2R)trix is a suppressor of cercus phenotype of Aobl\tra^fl.UAS, Scer\GAL4^da.G32

(Ruiz and Sanchez, 2010)

tra2^B/Df(2R)trix is a suppressor of abdominal tergite 8 phenotype of Aobl\tra^fl.UAS, Scer\GAL4^da.G32

(Ruiz and Sanchez, 2010)

NOT Suppressor of

Statement

Reference

tra2^B/Df(2R)trix is a non-suppressor of male genitalia phenotype of Aobl\tra^fl.UAS, Scer\GAL4^da.G32

(Ruiz and Sanchez, 2010)

tra2^B/tra2[+] is a non-suppressor of female genitalia phenotype of Aobl\tra^fl.UAS, Scer\GAL4^arm.PS

(Ruiz and Sanchez, 2010)

tra2^B/Df(2R)trix is a non-suppressor of sex comb phenotype of Aobl\tra^fl.UAS, Scer\GAL4^da.G32

(Ruiz and Sanchez, 2010)

tra2^B/Df(2R)trix is a non-suppressor of sex comb tooth phenotype of Aobl\tra^fl.UAS, Scer\GAL4^da.G32

(Ruiz and Sanchez, 2010)

Other

Statement

Reference

Df(2R)trix, Scer\GAL4^rn-GAL4-5, Scer\GAL80^ts.αTub84B, Scop\tra2^UAS.cMa, tra2^B has leg disc phenotype

(Martín et al., 2011)

Doa⁷, tra2^B has leg | female phenotype

(Du et al., 1998)

Doa⁷/Doa^unspecified, tra2^B has leg | female phenotype

(Du et al., 1998)

Doa^unspecified, tra2^B has leg | female phenotype

(Du et al., 1998)

fru¹, tra2^B has muscle cell of muscle of Lawrence phenotype

(Ryner et al., 1996)

Additional Comments

Genetic Interactions

Statement

Reference

XX tra2¹/tra2^B ; bon^S043420/bon^S043420 animals, that are genetically female (XX) but phenotypically male are viable.

(Beckstead et al., 2005)

The frequency of germline development (including spermatogenesis) in 2X tra2^B/Df(2R)trix males is unaffected by either snf¹ or otu^unspecified. 2X tra2^B/Df(2R)trix males that are also carrying ovo^unspecified do not show any evidence of germ cells.

(Andrews and Oliver, 2002)

XX ; tra2^B/+ adults that are also carrying Doa^unspecified/Doa⁷ are clearly intersexual with respect to the number or morphology of sex combs.

(Du et al., 1998)

fru¹ tra2^B double mutants have an abnormal Muscle of Lawrence, like that of fru¹ males.

(Ryner et al., 1996)

tra2^B/tra2⁺; tra^unspecified/tra⁺ female double heterozygotes are sometimes intersexual, particularly at high temperatures. Presence of B52^ED enhances the frequency of intersexuality.

(Peng and Mount, 1995)

Xenogenetic Interactions

Statement

Reference

The number of bristles seen in the sex comb on the first leg in XX tra2^B/Df(2R)trix pseudomales is significantly reduced by expression of Zzzz\Aobl-tra2^Scer\UAS.cSa under the control of Scer\GAL4^rn-GAL4 (the animals also contain the temperature sensitive Scer\GAL80^ts.αTub84B allele which is used to repress Scer\GAL4 expression until after hatching by raising the animals initially at 18[o]C and then transferring them to 25[o]C after hatching).

(Sarno et al., 2011)

Expression of Zzzz\tra^fl.Scer\UAS in a tra2^B/Df(2R)trix background, under the control of Scer\GAL4^da.G32 does not affect male morphology, indicating the endogenous tra2 protein is necessary in female development. Reducing, but not removing tra2, through expression of Zzzz\tra^fl.Scer\UAS in a tra2^B/+ background, under the control of Scer\GAL4^arm.PS leads to a significant reduction in the size of the female genital structures as well as a significant increase in the size of the male genital structures.

(Ruiz and Sanchez, 2010)

The mutant phenotype of XX ; tra2^B/Df(2R)trix animals is not rescued by Ccap\tra^hs.PP.

(Pane et al., 2005)

Female differentiation is variably restored in XX homozygotes carrying Dvir\tra2^tCa. Fertility can be restored in XY homozygotes by a single copy of Dvir\tra2^tCa.

(Chandler et al., 1997)

Complementation and Rescue Data

Rescued by

tra2^B is rescued by tra2^N.Tag:FLAG

(Dauwalder and Mattox, 1998)

tra2^B is rescued by tra2^{RS2.carb.N.Tag:FLAG}

(Dauwalder and Mattox, 1998)

Partially rescued by

tra2^B is partially rescued by tra2^C.lacZ

(Dauwalder and Mattox, 1998)

tra2^B is partially rescued by tra2^C.Tag:FLAG

(Dauwalder and Mattox, 1998)

tra2^B is partially rescued by tra2^ΔM1

(McGuffin et al., 1998)

Not rescued by

tra2^B is not rescued by tra2^{ΔRS.C.Tag:FLAG}

(Dauwalder and Mattox, 1998)

tra2^B is not rescued by tra2^{ΔRS.N.Tag:FLAG}

(Dauwalder and Mattox, 1998)

tra2^B is not rescued by tra2^ΔRS.C.lacZ

(Dauwalder and Mattox, 1998)

Comments

Images (0)

Mutant

Wild-type

Stocks (4)

Bloomington

25137

Dp(1;Y)Bar^S; cn¹ tra2^B bw¹/CyO

36296

y¹ w^*/Dp(1;Y)Bar^S; tra2^B/CyO; P{UAS-Soce\tra2}5

36297

P{UAS-Aobl\tra2}1, y¹ w^*/Dp(1;Y)Bar^S; tra2^B/CyO

36298

y¹ w^*/Dp(1;Y)Bar^S; tra2^B/CyO; P{UAS-Aobl\tra2}2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

tra-2^B

(Ruiz and Sanchez, 2010, Lazareva et al., 2007, Beckstead et al., 2005, Bielinska et al., 2005, Andrews and Oliver, 2002, Dauwalder and Mattox, 1998, McGuffin et al., 1998, Dauwalder et al., 1996, Mattox et al., 1996, Oliver et al., 1993)

tra-2B

(Sarno et al., 2011, Ryner et al., 1996, Wayne et al., 1995)

tra2^B

(Garner et al., 2018, Hudry et al., 2016, Martín et al., 2011)

tra2^b

(Qi et al., 2006, Du et al., 1998)

Name Synonyms

Secondary FlyBase IDs

References (33)

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