The FRT cassette has been excised, allowing expression of mutated form of wg (amino acid replacement G258D, the lesion present in wgNE1) under the control of UASt regulatory sequences.
Scer\GAL4e22c/wgNE1.UAS fails to rescue wgl-17
Fails to rescue the dorsal cuticle pattern of wgl-17 embryos when expressed under the control of Scer\GAL4e22c at 18oC.