FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\kniUAS.cCa
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General Information
Symbol
Dmel\kniUAS.cCa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Chen
FlyBase ID
FBal0094504
Feature type
allele
Associated gene
Dmel\kni
Associated Insertion(s)
Carried in Construct
P{UAS-kni.C}
Also Known As
UAS-kni
Key Links
Transgenic product class
cDNA
(Chen et al., 1998)
wild_type
(Chen et al., 1998)
Mutagen
Nature of the Allele
Transgenic product class
cDNA
(Chen et al., 1998)
wild_type
(Chen et al., 1998)
Mutagen
in vitro construct
(Chen et al., 1998)
Progenitor genotype
Carried in construct
P{UAS-kni.C}
Cytology
Description

Expression of kni cDNA is governed by UASt regulatory sequences.

(Chen et al., 1998)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
kni
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of kniScer\UAS.cCa under the control of Scer\GAL4btl.PS in the tracheal system results in the formation of fine dorsal trunks containing stretches of autocellular adherens junctions.

      (Ribeiro et al., 2004)

      kniScer\UAS.cCa driven by Scer\GAL4fkh.14-3 leads to a mild reduction in the BrdU incorporation domain in the large intestine.

      (Fuss et al., 2001)

      When expression is driven by Scer\GAL4btl.PS the severity of the Df(3L)ri-XT1 mutant tracheal phenotype is dramatically reduced. Embryos show restored dorsal, visceral and ganglionic branch outgrowths as well as lateral trunk formation. The ectopic expression does however cause reduced diameter and occasional interruption of the dorsal trunk. When expression is driven by two copies of Scer\GAL4btl.PS, dorsal trunk is absent and supernumerary cells migrate to dorsal positions, as observed for the ectopic expression of dpp.

      (Chen et al., 1998)

      When expression is driven by Scer\GAL4btl.PS the severity of the mutant tracheal phenotype is dramatically reduced. Embryos show restored dorsal, visceral and ganglionic branch outgrowths as well as lateral trunk formation. The ectopic expression does however cause reduced diameter and occasional interruption of the dorsal trunk. When expression is driven by two copies of Scer\GAL4btl.PS, dorsal trunk is absent and supernumerary cells migrate to dorsal positions, as observed for the ectopic expression of dpp.

      (Chen et al., 1998)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Enhanced by
      Enhancer of
      Suppressor of
      Statement
      Reference

      kniUAS.cCa/Scer\GAL4btl.PS is a suppressor of dorsal tracheal branch primordium phenotype of btl95

      (Myat et al., 2005)
      NOT Suppressor of
      Other
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Expression of kniScer\UAS.cCa, under the control of Scer\GAL4btl.PS, partially rescues the dorsal branch tracheal phenotype of btl95 mutant embryos but fails to rescue the lateral trunk defect and causes defects in dorsal trunk formation.

      (Myat et al., 2005)

      The combination of kniScer\UAS.cCa and knrlScer\UAS.cFa driven by Scer\GAL4fkh.14-3 has a strong effect on DNA replication in the hindgut. The BrDU is completely abolished. The large intestine region becomes reduced in size, although the cell number appears to be unaffected.

      The combination of kniScer\UAS.cCa and knrlScer\UAS.cFa driven by Scer\GAL4fkh.14-3 has a strong effect on DNA replication in the hindgut. The BrDU is completely abolished. The BrDU is completely abolished. The large intestine region becomes reduced in size, although the cell number appears to be unaffected.

      (Fuss et al., 2001)

      When expression is driven by Scer\GAL4btl.PS in a tkv mutant background, the ventral branch tracheal phenotype of tkv is not rescued, suggesting that dpp signalling though necessary is not sufficient for ventral branch formation.

      (Chen et al., 1998)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Partially rescues

      kniUAS.cCa/Scer\GAL4btl.PS partially rescues Df(3L)ri-XT1

      (Chen et al., 1998)
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      kniScer\UAS.cCa
      kniUAS.cCa
      Name Synonyms
      Saccharomyces cerevisiae UAS construct a of Chen
      (Chen et al., 1998)
      Secondary FlyBase IDs
        References (9)