FlyBase Allele Report: Dmel\btl[95]

General Information

Symbol

Dmel\btl⁹⁵

Species

D. melanogaster

Name

FlyBase ID

FBal0190429

Feature type

allele

Associated gene

Dmel\btl

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Myat et al., 2005)

Mutagen

ethyl methanesulfonate

(Myat et al., 2005)

Progenitor genotype

Cytology

Description

Amino acid replacement: D50N. Amino acid replacement: N181I.

(Myat et al., 2005)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3L:14,079,759..14,079,759 [+]

Nucleotide change:

G14079759A

Amino acid change:

D50N | btl-PA; D50N | btl-PB

Reported amino acid change:

D50N

Comment:

Site of nucleic acid difference inferred by FlyBase based on reported amino acid change. One of two amino acid substitutions in mutant.

(Myat et al., 2005)

point_mutation

3L:14,080,153..14,080,153 [+]

Nucleotide change:

A14080153T

Amino acid change:

N181I | btl-PA; N181I | btl-PB

Reported amino acid change:

N181I

Comment:

Site of nucleic acid difference inferred by FlyBase based on reported amino acid change. One of two amino acid substitutions in mutant.

(Myat et al., 2005)

point_mutation

3L:14,079,759..14,079,759 [+]

Nucleotide change:

G14079759A

Amino acid change:

D50N | btl-PA; D50N | btl-PB

Reported amino acid change:

D50N

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Myat et al., 2005)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

dorsal tracheal branch primordium

(Myat et al., 2005)

presumptive embryonic/larval tracheal system (with btl^H82Δ11)

(Myat et al., 2005)

presumptive embryonic/larval tracheal system (with btl^LG19)

(Myat et al., 2005)

presumptive embryonic/larval tracheal system (with Df(3L)fz-GF3b)

(Myat et al., 2005)

tracheal lateral trunk anterior branch primordium

(Myat et al., 2005)

Detailed Description

Statement

Reference

In btl⁹⁵ embryos, the migration of the dorsal branch and lateral trunk anterior is disrupted, but other tracheal branches are unaffected. When embryos are transheterozygous for btl⁹⁵ with btl^LG19, btl^H82Δ11, or Df(3L)fz-GF3b a more severe phenotype is seen in which migration of all primary tracheal branches is affected to varying degrees.

(Myat et al., 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressed by

Statement

Reference

btl⁹⁵ has dorsal tracheal branch primordium phenotype, suppressible by kni^UAS.cCa/Scer\GAL4^btl.PS

(Myat et al., 2005)

btl⁹⁵ has dorsal tracheal branch primordium phenotype, suppressible by Scer\GAL4^btl.PS/tkv^QD.UAS.cUa

(Myat et al., 2005)

btl⁹⁵ has dorsal tracheal branch primordium phenotype, suppressible by knrl^UAS.cLa/Scer\GAL4^btl.PS

(Myat et al., 2005)

NOT suppressed by

Statement

Reference

btl⁹⁵ has tracheal lateral trunk anterior branch primordium phenotype, non-suppressible by kni^UAS.cCa/Scer\GAL4^btl.PS

(Myat et al., 2005)

btl⁹⁵ has tracheal lateral trunk anterior branch primordium phenotype, non-suppressible by knrl^UAS.cLa/Scer\GAL4^btl.PS

(Myat et al., 2005)

btl⁹⁵ has tracheal lateral trunk anterior branch primordium phenotype, non-suppressible by Scer\GAL4^btl.PS/tkv^QD.UAS.cUa

(Myat et al., 2005)

Other

Statement

Reference

Scer\GAL4^btl.PS, btl⁹⁵, kni^UAS.cCa has tracheal dorsal trunk primordium phenotype

(Myat et al., 2005)

Scer\GAL4^btl.PS, btl⁹⁵, tkv^QD.UAS.cUa has tracheal dorsal trunk primordium phenotype

(Myat et al., 2005)

Scer\GAL4^btl.PS, btl⁹⁵, knrl^UAS.cLa has tracheal dorsal trunk primordium phenotype

(Myat et al., 2005)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of knrl^Scer\UAS.cLa, under the control of Scer\GAL4^btl.PS, rescues the dorsal branch tracheal phenotype of btl⁹⁵ mutant embryos, but causes defects in dorsal trunk formation. Expression of either kni^Scer\UAS.cCa or tkv^QD.Scer\UAS, driven by Scer\GAL4^btl.PS, partially rescues the dorsal branch tracheal phenotype, and also causes dorsal trunk formation. The lateral trunk defect of btl⁹⁵ embryos is not rescued by expression of any of these transgenes. kni⁶/+, btl⁹⁵/+ embryos show no tracheal defects.

Expression of knrl^Scer\UAS.cLa, under the control of Scer\GAL4^btl.PS, rescues the dorsal branch tracheal phenotype of btl⁹⁵ mutant embryos, but causes defects in dorsal trunk formation. Expression of either kni^Scer\UAS.cCa or tkv^QD.Scer\UAS, driven by Scer\GAL4^btl.PS, partially rescues the dorsal branch tracheal phenotype, and also causes defects in dorsal trunk formation. The lateral trunk defect of btl⁹⁵ embryos is not rescued by expression of any of these transgenes. kni⁶/+, btl⁹⁵/+ embryos show no tracheal defects.