FlyBase Allele Report: Dmel\knrl[UAS.cLa]

General Information

Symbol

Dmel\knrl^UAS.cLa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Lunde

FlyBase ID

FBal0092909

Feature type

allele

Associated gene

Dmel\knrl

Associated Insertion(s)

Carried in Construct

P{UAS-knrl.L}

Also Known As

UAS-knrl

Key Links

Transgenic product class

wild_type

(Lunde et al., 1998)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Lunde et al., 1998)

Mutagen

in vitro construct

(Lunde et al., 1998)

Progenitor genotype

Carried in construct

P{UAS-knrl.L}

Cytology

Description

The full length knrl coding region is expressed under the control of UASt regulatory sequences.

(Lunde et al., 1998)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

knrl

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

visible, with Scer\GAL4^Bx-MS1096

(Lunde et al., 1998)

Phenotype Manifest In

adult thorax & macrochaeta, with Scer\GAL4^Bx-MS1096

(Lunde et al., 1998)

embryo | dorsal closure stage, with Scer\GAL4^pnr-MD237

(Gritzan et al., 2002)

embryonic dorsal epidermis, with Scer\GAL4^pnr-MD237

(Gritzan et al., 2002)

metathoracic femur, with Scer\GAL4^Bx-MS1096

(Lunde et al., 1998)

Detailed Description

Statement

Reference

Expression of knrl^Scer\UAS.cLa under the control of Scer\GAL4^pnr-MD237 results in dorsal closure defects; embryos have a dorsal hole.

(Gritzan et al., 2002)

When expression is driven by Scer\GAL4^btl.PS the severity of the Df(3L)ri-XT1 mutant tracheal phenotype is dramatically reduced. Embryos show restored dorsal, visceral and ganglionic branch outgrowths as well as lateral trunk formation. The ectopic expression does however cause reduced diameter and occasional interruption of the dorsal trunk.

(Chen et al., 1998)

Flies expressing knrl^Scer\UAS.cLa under the control of Scer\GAL4^Bx-MS1096 often lack macrochaetae from the thorax and the femurs of the metathoracic legs are often twisted.

(Lunde et al., 1998)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

knrl^UAS.cLa/Scer\GAL4^kni.L2 is a suppressor of visible phenotype of kni^ri-1

(Lunde et al., 2003)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^pnr-MD237, knrl^UAS.cLa has embryo | dorsal closure stage phenotype, enhanceable by kay¹

(Gritzan et al., 2002)

Suppressed by

Statement

Reference

Scer\GAL4^pnr-MD237, knrl^UAS.cLa has embryo | dorsal closure stage phenotype, suppressible by puc^E69

(Gritzan et al., 2002)

Suppressor of

Statement

Reference

knrl^UAS.cLa/Scer\GAL4^btl.PS is a suppressor of dorsal tracheal branch primordium phenotype of btl⁹⁵

(Myat et al., 2005)

knrl^UAS.cLa/Scer\GAL4^kni.L2 is a suppressor of wing vein L2 phenotype of kni^ri-1

(Lunde et al., 2003)

NOT Suppressor of

Statement

Reference

knrl^UAS.cLa/Scer\GAL4^btl.PS is a non-suppressor of tracheal lateral trunk anterior branch primordium phenotype of btl⁹⁵

(Myat et al., 2005)

Other

Statement

Reference

Scer\GAL4^btl.PS, btl⁹⁵, knrl^UAS.cLa has tracheal dorsal trunk primordium phenotype

(Myat et al., 2005)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of knrl^Scer\UAS.cLa, under the control of Scer\GAL4^btl.PS, rescues the dorsal branch tracheal phenotype of btl⁹⁵ mutant embryos but fails to rescue the lateral trunk defect and causes defects in dorsal trunk formation.

(Myat et al., 2005)

Expression of knrl^Scer\UAS.cLa under the control of Scer\GAL4^kni.L2 rescues the L2 wing vein phenotype of kni^ri-1 flies.

(Lunde et al., 2003)

The dorsal closure phenotype caused by expression of knrl^Scer\UAS.cLa under the control of Scer\GAL4^pnr-MD237 is suppressed by puc^E69. The dorsal closure phenotype caused by expression of knrl^Scer\UAS.cLa under the control of Scer\GAL4^pnr-MD237 is enhanced by kay¹.

(Gritzan et al., 2002)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescues

knrl^UAS.cLa/Scer\GAL4^btl.PS partially rescues Df(3L)ri-XT1

(Chen et al., 1998)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

knrl^Scer\UAS.cLa

knrl^UAS.cLa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Lunde

(Lunde et al., 1998)

Secondary FlyBase IDs

References (8)

Report Sections

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