FlyBase Allele Report: Dmel\C3G[UAS.Tag:MYC,Tag:M(c-SRC)]

General Information

Symbol

Dmel\C3G^{UAS.Tag:MYC,Tag:M(c-SRC)}

Species

D. melanogaster

Name

FlyBase ID

FBal0094887

Feature type

allele

Associated gene

Dmel\C3G

Associated Insertion(s)

Carried in Construct

P{UAS-C3G.T:Myc.T:Myr3}

Key Links

Transgenic product class

wild_type

(Ishimaru et al., 1999)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Ishimaru et al., 1999)

Mutagen

in vitro construct

(Ishimaru et al., 1999)

Progenitor genotype

Carried in construct

P{UAS-C3G.T:Myc.T:Myr3}

Cytology

Description

Full length C3G tagged with 6 copies of the Tag:MYC tag is expressed under the control of UASt regulatory sequences. The C3G sequences are also tagged at the 5' end with Tag:M(c-SRC), to produce a membrane-targetted protein.

(Ishimaru et al., 1999)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

C3G

Tagged with

Tag:M(c-SRC)

membrane protein localization tag

Tag:MYC

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

eye, with Scer\GAL4^GMR.PF

(Ishimaru et al., 1999)

eye photoreceptor cell, with Scer\GAL4^GMR.PF

(Ishimaru et al., 1999)

interommatidial bristle, with Scer\GAL4^GMR.PF

(Ishimaru et al., 1999)

larval somatic muscle cell, with Scer\GAL4^Mef2.PR

(Shirinian et al., 2010)

lens, with Scer\GAL4^GMR.PF

(Ishimaru et al., 1999)

ommatidium, with Scer\GAL4^GMR.PF

(Ishimaru et al., 1999)

pigment cell, with Scer\GAL4^GMR.PF

(Ishimaru et al., 1999)

rhabdomere, with Scer\GAL4^GMR.PF

(Ishimaru et al., 1999)

ventral longitudinal muscle cell, with Scer\GAL4^Mef2.PR

(Shirinian et al., 2010)

wing disc, with Scer\GAL4^dpp.blk1

(Ishimaru et al., 1999)

wing vein L3, with Scer\GAL4^dpp.blk1

(Ishimaru et al., 1999)

Detailed Description

Statement

Reference

C3G^{Scer\UAS.T:Hsap\MYC,T:Ggal\Myr3} driven by Scer\GAL4^Mef2.PR at 25C results in embryonic lethality and severe muscle defects are seen during the embryonic stages compared to controls.

C3G^{Scer\UAS.T:Hsap\MYC,T:Ggal\Myr3} driven by Scer\GAL4^Mef2.PR at 18C allows development of larvae. The larvae display severe morphological muscle defects, disruption of actin organisation in somatic muscles and detachment of the ventral longitudinal muscles compared to controls.

(Shirinian et al., 2010)

Flies expressing C3G^{Scer\UAS.T:Hsap\MYC,T:Ggal\Myr3} under the control of Scer\GAL4^GMR.PF have a rough eye phenotype with varying degrees of severity depending on the P{UAS-C3G.T:Myc.T:Myr3} insertion line used. Flies with an intermediate phenotype have a moderately rough eye surface with differentiated lenses and bristles. All ommatidia contain supernumerary photoreceptor cells, with up to 5 or 6 additional cells in some cases. Most of these extra photoreceptor cells have small rhabdomeres and are located in the distal portion of the retina, suggesting that they are R7 cells. Some pigment cells are present, but they fail to form a lattice surrounding the ommatidia. Flies with a strong phenotype have reduced eyes and the surface appears rough and folded. There are very few bristles on the surface of the eye and lens facets are not discernible. The ommatidial lattice is severely disrupted; fewer clusters of photoreceptor cells are seen than normal, the number of cells per cluster and the arrangement of the cells is irregular and the rhabdomeres often do not span the entire thickness of the retina and have an elongated morphology. Wing discs expressing C3G^{Scer\UAS.T:Hsap\MYC,T:Ggal\Myr3} under the control of Scer\GAL4^dpp.blk1 show an increase in BrdU incorporation in a band corresponding to the domain of Scer\GAL4^dpp.blk1 expression. Adult wings of flies expressing C3G^{Scer\UAS.T:Hsap\MYC,T:Ggal\Myr3} under the control of Scer\GAL4^dpp.blk1 show a massive broadening of the third vein.