Genomic nanos fragment in which the nanos 3'UTR has been replaced with the αTub84B 3'UTR. Two direct repeats of the +2' element from the nanos 3' untranslated region (UTR) have been inserted in the αTub84B 3' UTR sequences. A Tag:HA epitope has been inserted at the 5' end of the nanos coding region.
Embryos derived from vas1/vas3 females carrying nosnos.+2'-2X.T:Ivir\HA1 develop 0-3 abdominal segments in 16% of cases, 4-6 abdominal segments in 51% of cases and 7-8 abdominal segments in 34% of cases.
nanosnanos.+2'-2X.Tag:HA partially rescues nanosBN
Embryos derived from nosBN females carrying nosnos.+2'-2X.T:Ivir\HA1 develop 4-6 abdominal segments in 26% of cases and 7-8 abdominal segments in 70% of cases.