Lar^bola1/Lar^bola2 transheterozygous eggs are rounder than controls (Lar^bola1/+, Lar^bola2/+ and wild-type). Follicular epithelium stress fibers are highly misaligned in stage 5 egg chambers, but become less misaligned at stage 7, as compared to wild-type and Lar^bola2 heterozygous controls.

In Lar^bola2 heterozygotes, follicular epithelium migration is severely increased in stage 7 egg chambers, as compared to controls.

Lar^bola1/Lar^bola2 follicular epithelia show a fully penetrant yet variable defect in their migration motility, as compared to controls, with around half of these mutant epithelia exhibiting no migration, while the rest migrate slower than controls.

In mutant females 20-70% of the follicles fail to elongate, despite transferring their nurse cell contents normally. Unelongated follicles develop into eggs that are nearly round, resembling a football (sensu UK) and contain unelongated follicle cells, but enclose a normal volume. Stage 10A bola follicles reveal normal actin microfilament bundles in their nurse cells. Their basal actin filaments are indistinguishable from wild-type in the germarium and stage 2-4 egg chambers. However in 30-90% of follicles, the filaments, the filaments never become aligned perpendicular to the anterior posterior axis, although intracellularly, the number and size of actin bundles appear normal. Nearly half of mutant follicles contain an average of 2.3 anterior polar cells and 2.6 posterior polar cells (compared to two pairs in wild-type). Moreover 3.3% of the follicles contain an ectopic polar cell cluster from 2-12 cells. All the follicles with extra pole cells, whether located at the poles or ectopically, fail to undergo actin alignment. However the converse is not true. Many follicles are observed in which the number and location of polar cells is normal, but the actin fibres are not aligned. Germaria from mutant females are frequently found top be abnormal. In 57% of these germaria the cysts in region 2b remain two-across and never become lens shaped. Egg chamber budding is slowed and occurs abnormally, frequently causing additional round cysts to accumulate in region 3 of the germarium, and occasionally producing composite follicles containing two cysts. Although mutants oocytes never initiate embryonic development, anterior posterior polarity is seen do be disrupted (as seen by osk localisation).

Mutants produce round eggs. The organisation of the cytoskeleton within the follicle cells is disrupted; the actin bundles are still aligned within each cell, but these bundles are not always perpendicular to the anterior/posterior axis, as seen in wild type.

Lar^bola2/Lar[+] is an enhancer of abnormal size | oogenesis phenotype of PlexA^HM05221, Scer\GAL4^NP1624

Lar^bola2/Lar[+] is an enhancer of visible | oogenesis phenotype of PlexA^HM05221, Scer\GAL4^NP1624

Lar^bola2/Lar[+] is a suppressor of abnormal size | oogenesis phenotype of Sema5c^K175

Lar^bola2/Lar[+] is a suppressor of visible | oogenesis phenotype of Sema5c^K175

Lar^bola2/Lar[+] is a suppressor | partially of follicle cell phenotype of Sema5c^K175

Lar^bola2/Lar[+] is a suppressor of egg phenotype of Sema5c^K175

Lar^bola2/Lar[+] is a suppressor | partially of stress fiber | oogenesis phenotype of Sema5c^K175