Construct: The Hsp83 degradation element (HDE) from the Hsp83 3' untranslated region (nucleotides 253 to 349 of the 3' UTR) is inserted between the end of the nos open reading frame and the nos 3' UTR sequences.
Embryos carrying nosΔTCE+HDE have severe defects in the head skeleton, including loss of the dorsal bridge, dorsal arms, vertical plates and ventral arms.