FlyBase Allele Report: Dmel\pck[G0012]

(Harder et al., 2008, Luschnig et al., 2006, Moussian et al., 2006, Behr et al., 2003, Peter et al., 2002, Schäfer et al., 1999.6.15, Schäfer et al., 1999.8.12)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

AJ300012

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal | recessive

(Schäfer et al., 1999.6.15, Schäfer et al., 1999.8.12)

lethal - all die during embryonic stage

(Peter et al., 2002)

some die during embryonic stage

(Peter et al., 2002)

Phenotype Manifest In

Detailed Description

Statement

Reference

Mutant embryos have elongated trachea.

(Nelson et al., 2010)

pck^G0012 mutant embryos are characterised by an elongated and tortuous dorsal trunk, the main trachea, and by irregular and stretched dorsal trunk cells.

(Harder et al., 2008)

The dorsal trunk of pck^G0012 homozygous embryos exhibits an elongated and convoluted lumen. The tracheal pericellular-diffusion barrier is compromised in pck^G0012 mutants.

(Luschnig et al., 2006)

In pck^G0012 mutants, the lumenal chitin in the developing dorsal trunk of the tracheal system fails to form a filamentous cable running along the tube length, as in wild-type, but instead is amorphous and fills the lumen.

(Tonning et al., 2005)

Elongated and tortuous tracheal branches, most prominantly in the dorsal trunk. Pleated septate junctions are absent from tracheal cells; intercellular cleft is instead filled with unstructured electron-dense material. Germline clone analysis revealed that embryos lacking maternal pck function form a normal tracheal system and develop into fertile adults. Zygotic mutant phenotype is indistinguishable from that of embryos lacking both maternal and zygotic component pck. Transepithelial barrier function is affected - rhodamine labelled dextran diffuses across the epithelial sheet.

(Behr et al., 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

The tracheal length defects of kune^C309 pck^G0012 sinu^nwu7 triple mutant embryos are no more severe than those of single mutants.

(Nelson et al., 2010)

Xenogenetic Interactions

Statement

Reference

Co-expression of Zzzz\NIa^Scer\UAS.cHa under the control of Scer\GAL4^btl.PS produces tracheal defects in flies expressing pck^{T:Zzzz\TEV.CS} in a pck^G0012 mutant background, phenocopying the pck^G0012 mutant phenotype.

(Harder et al., 2008)

Complementation and Rescue Data

Fails to complement

pck^VE896

(Behr et al., 2003)

pck^EA97

(Behr et al., 2003)

Rescued by

pck^G0012 is rescued by pck^Tag:CS(TEVp)

(Harder et al., 2008)

Comments

Expression of pck^{T:Zzzz\TEV.CS} rescues the tracheal phenotypes seen in pck^G0012 mutant embryos.

(Harder et al., 2008)

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

11471

y¹ P{lacW}pck^G0012 w^67c23/FM7c

Kyoto

111760

y¹ P{lacW}pck^G0012 w^* P{neoFRT}19A/FM7c; P{ey-FLP.N}5

Notes on Origin

Discoverer

Comments

P{lacW} not verified as causing the lethality.

(Schäfer et al., 1999.6.15, Schäfer et al., 1999.8.12)

Mobilisation of the P{lacW} causes reversion of the mutant phenotype.

(Behr et al., 2003)

External Crossreferences and Linkouts ( 1 )

Crossreferences

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

AJ300012

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

l(1)G0012

(Peter et al., 2002)

l(1)G0012^G0012

(Schäfer et al., 1999.6.15, Schäfer et al., 1999.8.12)

mega¹²

(Harder et al., 2008)

mega^G0012

(Drees et al., 2023, Behr and Riedel, 2020, Tempesta et al., 2017, Hildebrandt et al., 2015, Bätz et al., 2014, Nelson et al., 2010, Luschnig et al., 2006, Tonning et al., 2005, Behr et al., 2003)

pck^G0012

(Hall et al., 2014, Tonning et al., 2005)

Name Synonyms

Secondary FlyBase IDs

References (15)

Report Sections

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