G-oα47A007 wing somatic clones exhibit mutant margin bristles. G-oα47A007 thoracic mutant clones exhibit bald regions with surviving bristles displaying polarity defects. Defects, due to aberrations in asymmetric division, including the absence of external cells, duplicated sockets and/or hairs, and bristles devoid of sockets or hairs are observed in G-oα47A007 mutant clones. Approximately 8% of G-oα47A007 bristles exhibit hair/socket duplications. Socket-only phenotypes occur in 5% of cases.
Only late induced somatic clones survive into the adult. Although homozygous early induced clones in the wing do not survive to the adult stage, clear polarity defects are seen in these wings in the adult, likely as a result of nonautonomous effects of clones that subsequently died. Homozygous late induced clones in the wing induce the loss of wing margin. In addition, these late induced clones often secrete multiple wing hairs, with up to five hairs per cell. These clones often cause nonautonomous defects on their proximal side. In addition, clones in the wing margin also result in nonautonomous defects in wing margin bristle polarity on the proximal side of the clone.
Homozygous embryos have defects in the heart and visceral muscles. Interruptions are seen in the dorsal vessel. Cardial cells are present and migrate properly to a dorsal position, but in some places they are not arranged as a continuous layer but appear as unorganised clusters of cells. The same types of defects are seen in the visceral mesoderm. Longitudinal axons of the nervous system are often pinched or sometimes missing and the axons of motoneurons are clearly misrouted.
Gαo007/G-oalpha47A[+], Octβ1R1 has abnormal neuroanatomy | dominant | third instar larval stage phenotype
Gαiunspecified, Gαo007 has abnormal planar polarity | somatic clone phenotype
Gαiunspecified, Gαo007 has abnormal cell polarity | somatic clone phenotype
Gαo007/G-oalpha47A[+], Octβ1R1 has type I bouton | increased number | third instar larval stage phenotype
Gαo007/G-oalpha47A[+], Octβ1R1 has embryonic/larval neuromuscular junction | third instar larval stage phenotype
Gαo007/G-oalpha47A[+], Octβ1R1 has type II bouton | increased number | third instar larval stage phenotype
Gαiunspecified, Gαo007 has macrochaeta | somatic clone phenotype
Gαiunspecified, Gαo007 has wing | somatic clone phenotype
Gαiunspecified, Gαo007 has eo neuron | somatic clone phenotype
Gαiunspecified, Gαo007 has eo sensory structure | somatic clone phenotype
Gαiunspecified, Gαo007 has sensory organ cell | somatic clone phenotype
Gαiunspecified, Gαo007 has tormogen cell | somatic clone phenotype
G-oα47A007/+ ; oa21/+ double heterozygous third instar larvae show a significant increase in the number of natural synaptopods (motile filopodia-like extensions) and type II boutons at the type II neuromuscular junction compared to wild type. The number of type I boutons at the neuromuscular junction is also increased in these animals.
G-oα47A007 heterozygotes in a G-iα65Aunspecified homozygous background exhibit adult bristle defects that are not present in G-iα65Aunspecified homozygotes.
Gαo007 is partially rescued by Scer\GAL4how-24B/GαoII.UAS