FB2026_02 , released June 18, 2026
Allele: Dmel\Syx1AI212A
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General Information
Symbol
Dmel\Syx1AI212A
Species
D. melanogaster
Name
FlyBase ID
FBal0099811
Feature type
allele
Associated gene
Dmel\Syx1A
Associated Insertion(s)
Carried in Construct
P{syx1AI212A}
Key Links
Transgenic product class
missense_variant
(Wu et al., 1999)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Wu et al., 1999)
Mutagen
in vitro construct
(Wu et al., 1999)
Progenitor genotype
Carried in construct
P{syx1AI212A}
Cytology
Description

13.5kb XbaI genomic fragment that encompasses Syx1A. A I212A amino acid replacement has been introduced into the Syx1A coding sequence (the mutation is within the region of the H3 domain thought to be required for Snap25 binding).

(Wu et al., 1999)
Allele components
Component
Use(s)
Regulatory region(s)
Syx1A
Encoded product / tool
Syx1A
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Syx1AΔ229 and Syx1A06737 flies rescued with Syx1A+t13.5, like wild-type, undergo peristaltic muscle contractions prior to hatching. They also show a robust response to tactile stimulation. Syx1AΔ229 embryos rescued with Syx1AI212A exhibit wild-type cuticle phenotypes. Using whole-cell patch-clamp analysis, Syx1AI212A in a Syx1AΔ229 null background shows a very significantly enhanced evoked junctional current amplitude compared to Syx1A+t13.5 controls (over 200% of wild-type amplitude). There is no significant change in the amplitude of spontaneous mEJCs. The mEJC frequency is also greatly increased. In the absence of Ca2+, this frequency is halved, though still significantly higher than wild-type.

      (Wu et al., 1999)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      Syx1AI212A rescues Syx1A06737

      (Wu et al., 1999)
      Partially rescues

      Syx1AI212A partially rescues Syx1AΔ229

      (Wu et al., 1999)
      Comments

      Rescues Syx1AΔ229 to 1st instar larval stage. Rescues Syx1A06737 to adult viability.

      (Wu et al., 1999)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Syx1AI212A
      syx1AI212A
      syx2
      (Wu et al., 1999)
      Name Synonyms
      Secondary FlyBase IDs
        References (1)