FlyBase Allele Report: Dmel\lqf[S011027]

General Information

Symbol

Dmel\lqf^S011027

Species

D. melanogaster

Name

FlyBase ID

FBal0100180

Feature type

allele

Associated gene

Dmel\lqf

Associated Insertion(s)

P{lacW}lqf^S011027

Carried in Construct

Also Known As

l(3)0110/27

Key Links

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Delta2-3 transposase

(Deak et al., 1997)

P-element activity

(Szeged Stock Center, 1998-)

Progenitor genotype

Associated Insertion(s)

P{lacW}lqf^S011027

Cytology

Description

P{lacW}lqf^S011027 is inserted at the 5' end of lqf approximately 32bp downstream of the transcription start site of the lqf-RC, -RB, -RE-, and -RF transcripts.

(Csikós et al., 2009)

Insertion in the first intron of lqf.

(Tian et al., 2004)

The P{lacW} insertion is upstream of lqf^S011027 coding region.

(Overstreet et al., 2003)

P{lacW} insertion either in the 5' UTR or within the promoter.

(Cadavid et al., 2000)

Allele components

Component

Use(s)

Inserted element

P{lacW}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal (with Df(3L)pbl-X1)

(Deak et al., 1997)

lethal | recessive

(Cadavid et al., 2000, Szeged Stock Center, 1998-)

lethal - all die before end of pupal stage | recessive

(Deak et al., 1997)

some die during pupal stage | recessive

(Deak et al., 1997)

Phenotype Manifest In

dorsal vessel wall cell | increased number

(Tian et al., 2004)

embryonic/larval fat body

(Csikós et al., 2009)

mitochondrial outer membrane

(Csikós et al., 2009)

Detailed Description

Statement

Reference

The development of heterozygous lqf^S011027 larvae shows practically no differences compared to that of wild-type animals. There is no difference between the diameter of the trophocytes or their nucleus size between homozygous and heterozygous lqf^S011027 larvae. Despite these similarities, the fat body of lqf^S011027 homozygous larvae does not form any acridine orange or neutral red positive granules that correspond to the autophagic and/or endocytic (heterophagic) vacuoles.

Fat body cells of lqf^S011027 larvae are unable to form large autophagosomes and autolysosomes. However, small lysosomes and autophagosomes can be observed in the cytoplasm of the trophocytes. Multivesicular bodies and protein storage granules are also absent from the fat body cells of the mutant animals during the wandering period. The size of the lipid droplets and glycogen areas are smaller than in control animals and the rough endoplasmic reticulum is often fragmented into vesicles. The outer membrane of the mitochondria is thicker and more electron dense than those of the controls.

(Csikós et al., 2009)

Homozygous embryos have an excess of cardioblasts.

(Tian et al., 2004)

lqf^FDD9/lqf^S011027 flies have similar but more severe phenotypes than those of lqf^FDD9 homozygotes.

(Cadavid et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Allelic series based on viability: lqf^FDD9 < lqf^bE248 < lqf^S011027 < lqf^BT < lqf^ARI = lqf^L895 = lqf^L71.

(Overstreet et al., 2003)

Allelic series of phenotypic severity: lqf^ARI >= lqf^AG > lqf^bE25 > lqf^bE248 > lqf^S011027.

(Tian et al., 2004)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (8)

Reported As

Symbol Synonym

0110/27

(Deak et al., 1997)

011027

(Overstreet et al., 2003)

l(3)0110/2

(Tian et al., 2004)

l(3)0110/27

(Tian et al., 2004, Cadavid et al., 2000)

l(3)S011027^S011027

(Szeged Stock Center, 1998-)

lqf^P011027

(Cadavid et al., 2000)

lqf^S011027

lqf^SO11027

(Fleming et al., 2013)

Name Synonyms

Secondary FlyBase IDs

References (7)

Report Sections

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